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Method of Inducing a Phase Transition of a Bilayer Membrane Vesicle

外国特許コード F140007821
整理番号 K05331US2
掲載日 2014年1月22日
出願国 アメリカ合衆国
出願番号 201313870002
公報番号 20130251788
出願日 平成25年4月25日(2013.4.25)
公報発行日 平成25年9月26日(2013.9.26)
国際出願番号 JP2005023196
国際公開番号 WO2006080157
国際出願日 平成17年12月12日(2005.12.12)
国際公開日 平成18年8月3日(2006.8.3)
優先権データ
  • 特願2005-021241 (2005.1.28) JP
  • 11/814,901 (2007.7.26) US
  • 2005JP023196 (2005.12.12) WO
発明の名称 (英語) Method of Inducing a Phase Transition of a Bilayer Membrane Vesicle
発明の概要(英語) Provided is a bilayer membrane vesicle capable of undergoing a phase transition.
The bilayer membrane vesicle includes: (a) a fatty acid salt having 6 to 20 carbon atoms; (b) an alcohol or an amine compound having an aliphatic chain of 6 to 20 carbon atoms; and (c) an artificial synthetic lipid or a phospholipid capable of forming a bilayer membrane.
Preferably, this bilayer membrane vesicle further contains (d) a tertiary amine as a component of the membrane.
Also provided is a method of inducing a phase transition of a bilayer membrane vesicle, the method including the step of adding a dehydrating condensing agent or a dehydrating condensing agent precursor having the property of accumulating at an interface to the bilayer membrane vesicle.
By causing the lipids that form a molecular aggregate to chemically change, it is possible to change the physical property and the morphology of the molecular aggregate and control the timing of phase transitions such as membrane fusion.
In the membrane fusion, for example, fusion can occur without leakage of the contents of the bilayer membrane vesicle.
特許請求の範囲(英語) [claim1]
1. A method of inducing a phase transition of a bilayer membrane vesicle, comprising: preparing a bilayer membrane vesicle, in which the bilayer membrane vesicle comprises as components of the membrane:
(a) a fatty acid salt having 6 to 20 carbon atoms;
(b) an alcohol or an amine compound having an aliphatic chain of 6 to 20 carbon atoms; and
(c) an artificial synthetic lipid or a phospholipid capable of forming a bilayer membrane; and
adding a dehydrating condensing agent or a dehydrating condensing agent precursor to the bilayer membrane vesicle.
[claim2]
2. The method of claim 1, wherein the (b) alcohol or amine compound is a dihydric alcohol represented by the following formula I:
R1 -- NH -- CH2 -- CH(OH) -- CH2OH (I)
wherein R1 is an alkyl group having 6 to 20 carbon atoms, an alkenyl group having 6 to 20 carbon atoms, or an alkynyl group having 6 to 20 carbon atoms.
[claim3]
3. The method of claim 1, wherein the bilayer membrane vesicle further comprises as a component of the membrane: (d) a tertiary amine represented by the following formula II:
wherein one or two of R2, R3, and R4 is a methyl group, and the remaining R2, R3, and R4 is each independently -- CH2COOCnH2n+1, -- CnH2n+1, or -- C6H4-p-CnH2n+1, where n is an integer of 6 to 20, and -- CnH2n+1 is linear; and
wherein the dehydrating condensing agent precursor is a cyanuric acid derivative represented by the following formula III:
wherein R5 and R6 are each independently a methyl group, an ethyl group, a hydroxyalkyl group having 2 to 5 carbon atoms, -- (CH2CH2O)mR7 (where m is an integer from 1 to 120, and R7 is a hydrogen atom, a methyl group, an ethyl group, or a propyl group), -- (CH2CH2NR8)mH (where m is an integer of 1 to 120, and R8 is an alkyl group having 2 to 5 carbon atoms, an N,N-dialkylaminoethyl group, or -- CH2CH2N+(CH3)3), -- CH2CH2SO3-, -- CH2CH2N+(CH3)3, or an alkyl group having 6 to 20 carbon atoms, but both R5 and R6 are not an alkyl group having 6 to 20 carbon atoms at the same time; and X is a halogen atom.
[claim4]
4. The method of claim 3, wherein at least one of R5 and R6 in the formula III is a methyl group or an ethyl group.
[claim5]
5. The method of claim 3, wherein n in the formula II is 12 to 16.
[claim6]
6. The method of claim 2, wherein the bilayer membrane vesicle further comprises as a component of the membrane: (d) a tertiary amine represented by the following formula II:
wherein one or two of R2, R3, and R4 is a methyl group, and the remaining R2, R3, and R4 is each independently -- CH2COOCnH2n+1, -- CnH2n+1, or -- C6H4-p-CnH2n+1, where n is an integer of 6 to 20, and -- CnH2n+1 is linear; and
wherein the dehydrating condensing agent precursor is a cyanuric acid derivative represented by the following formula III:
wherein R5 and R6 are each independently a methyl group, an ethyl group, a hydroxyalkyl group having 2 to 5 carbon atoms, -- (CH2CH2O)mR7 (where m is an integer from 1 to 120, and R7 is a hydrogen atom, a methyl group, an ethyl group, or a propyl group), -- (CH2CH2NR8)mH (where m is an integer of 1 to 120, and R8 is an alkyl group having 2 to 5 carbon atoms, an N,N-dialkylaminoethyl group, or -- CH2CH2N+(CH3)3), -- CH2CH2SO3-, -- CH2CH2N+(CH3)3, or an alkyl group having 6 to 20 carbon atoms, but both R5 and R6 are not alkyl group having 6 to 20 carbon atoms at the same time; and X is a halogen atom.
[claim7]
7. The method of claim 4, wherein n in the formula II is 12 to 16.
  • 発明者/出願人(英語)
  • KUNISHIMA MUNETAKA
  • JAPAN SCIENCE & TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 424/450
  • 435/29
  • 435/375
参考情報 (研究プロジェクト等) PRESTO Conversion and Control by Advanced Chemistry AREA
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