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Method for producing bilayer membrane and planar bilayer membrane コモンズ

外国特許コード F140007864
整理番号 06T171P/US
掲載日 2014年4月24日
出願国 アメリカ合衆国
出願番号 45051708
公報番号 20100148123
公報番号 8961898
出願日 平成20年3月28日(2008.3.28)
公報発行日 平成22年6月17日(2010.6.17)
公報発行日 平成27年2月24日(2015.2.24)
国際出願番号 JP2008056737
国際公開番号 WO2008120816
国際出願日 平成20年3月28日(2008.3.28)
国際公開日 平成20年10月9日(2008.10.9)
優先権データ
  • 07JP-0094443 (2007.3.30) 20
  • 08WO-JP56737 (2008.3.28) 20
発明の名称 (英語) Method for producing bilayer membrane and planar bilayer membrane コモンズ
発明の概要(英語) (US8961898)
The present invention provides a bilayer membrane produced using a microchannel capable of easily forming bilayer membranes such as planar lipid bilayer membranes in large quantities, and a production method thereof.
A process for producing a bilayer membrane of the present invention comprises forming a state where two liquid phases or liquid and gaseous phases each containing amphipathic molecules are alternately arranged in a microchannel, discharging one of the two liquid phases or the gaseous phase of the liquid and gaseous phases through branch minichannels formed in the wall on one side or in the walls on both sides to contact the remaining liquid phases adjacent to each other, and thereby forming a side-by-side arrangement of bilayer membranes comprising the amphipathic molecules.
特許請求の範囲(英語) [claim1]
1. A process for producing planar bilayer membranes, comprising: forming a state where two liquid phases or liquid and gaseous phases each containing amphipathic molecules are alternately arranged as an array in a microchannel,
discharging one of the two liquid phases or the gaseous phase of the liquid and gaseous phases from the microchannel through branch minichannels formed in the wall on one side or in the walls on both sides of the microchannel, so as to contact the remaining liquid phases adjacent to each other, and
forming a side-by-side arrangement of planar bilayer membranes comprising the amphipathic molecules, wherein the planar bilayer membranes are independent from each other.
[claim2]
13. An apparatus comprising a microchannel, and two or more planar bilayer membranes which are arranged as an array in said microchannel and contain amphipathic molecules, wherein each of the bilayer membranes contacts an organic phase or a water phase at both sides of the membrane, and wherein each of the bilayer membranes is sandwiched by mutually independent organic phases or water phases.
[claim3]
2. A process for producing bilayer membranes according to claim 1, comprising: alternately introducing the two liquid phases or liquid and gaseous phases each containing amphipathic molecules using a branch structure of the microchannel to form the state where the two liquid phases or liquid and gaseous phases each containing amphipathic molecules are alternately arranged.
[claim4]
3. A process for producing bilayer membranes according to claim 2, wherein the branch structure of a microchannel is selected from a cross-shaped intersection, a T-shaped intersection, and a Y-shaped intersection.
[claim5]
4. A process for producing bilayer membranes according to claim 1, wherein the two liquid phases are an organic phase and a water phase.
[claim6]
5. A process for producing bilayer membranes according to claim 1, wherein the amphipathic molecules are a lipid, surfactant, or polymer.
[claim7]
6. A process for producing bilayer membranes according to claim 4, wherein the amphipathic molecules are introduced in admixture with the organic phase.
[claim8]
7. A process for producing bilayer membranes according to claim 1, wherein the amphipathic molecules are adsorbed on an interface of the organic phase and the water phase to form a monolayer membrane.
[claim9]
8. A process for producing bilayer membranes according to claim 4, comprising discharging the organic phase through branch minichannels formed in the wall on one side or in the walls on both sides of the microchannel, thereby bringing the adjacent water phases in contact with each other.
[claim10]
9. A process for producing bilayer membranes according to claim 4, comprising discharging the water phase through branch minichannels formed in the wall on one side or in the walls on both sides of the microchannel, thereby bringing the adjacent organic phases in contact with each other.
[claim11]
10. A process for producing bilayer membranes according to claim 1, wherein the discharge through branch minichannels is performed by capillary action through the branch minichannels and/or suction.
[claim12]
11. A process for producing bilayer membranes according to claim 1, wherein two or more bilayer membranes have the side-by-side arrangement at a constant interval.
[claim13]
12. A process for producing bilayer membranes according to claim 1, wherein the bilayer membranes are sandwiched with liquid phases having components different from each other.
[claim14]
14. The apparatus according to claim 13, wherein each of the bilayer membranes is sandwiched by liquid phases having components different from each other.
[claim15]
15. The apparatus according to claim 13, wherein the amphipathic molecules are a phosphatide.
[claim16]
16. The apparatus according to claim 15, wherein a biological molecule is immobilized in the phosphatide.
[claim17]
17. The apparatus according to claim 16, wherein the biological molecule is a membrane protein.
[claim18]
18. The apparatus according to claim 17, wherein the membrane protein is selected from one or more of ion channel proteins, transporters, ion pump proteins, and receptors.
[claim19]
19. A device comprising the apparatus according to claim 15.
[claim20]
20. A device according to claim 19, wherein the device is adapted for a function analysis of membrane proteins.
[claim21]
21. A device according to claim 19, wherein the device is a sensor device.
  • 発明者/出願人(英語)
  • NISISAKO TAKASI
  • BABA TAKAHIRO
  • TOKYO INSTITUTE OF TECHNOLOGY
国際特許分類(IPC)
米国特許分類/主・副
  • 422/502.0

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