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METHOD FOR CLONING T CELL RECEPTOR

外国特許コード F140007957
整理番号 S2012-0940-C0
掲載日 2014年9月26日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2013JP070028
国際公開番号 WO 2014017533
国際出願日 平成25年7月24日(2013.7.24)
国際公開日 平成26年1月30日(2014.1.30)
優先権データ
  • 特願2012-164442 (2012.7.25) JP
発明の名称 (英語) METHOD FOR CLONING T CELL RECEPTOR
発明の概要(英語) The present invention addresses the problem of providing a T cell receptor (TCR) cloning system which enables the analysis of non-biased TCR repertories, enables the collection of an antigen-specific (TCR α cDNA)/( TCR β cDNA) pair, and also enables the evaluation of the function of the pair. Provided is a method for producing a TCR gene specific to an antigen (A), comprising the steps of: (1) stimulating a group of T cells which contains antigen-(A)-specific T cells or a single antigen-(A)-specific T cell under the conditions effective for the amplification of the TCR gene; (2) identifying the antigen-(A)-specific T cells among from the group of T cells which contains the antigen-(A)-specific T cells and then sorting the identified antigen-(A)-specific T cells into a container one by one; and (3) subjecting one of the activated antigen-(A)-specific T cells in the container to PCR to amplify the TCR gene specific to the antigen (A). According to the present invention, it becomes possible to clone a TCR gene of interest in a shorter time compared with the conventional methods, e.g., within about 10 days. According to the present invention, it also becomes possible to clone a gene for TCR α-chain and a gene for TCR β-chain with high efficiency. Under the conditions employed in examples, a (TCR α-chain)-(TCR β-chain) pair was able to be obtained with an efficiency of 100% from the T cells which had been sorted one by one after stimulation.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF TOYAMA
  • 発明者(英語)
  • KISHI HIROYUKI
  • MURAGUCHI ATSUSHI
  • KOBAYASHI EIJI
  • OZAWA TATSUHIKO
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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