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POLYPEPTIDE FOR FORSTER RESONANCE ENERGY TRANSFER

外国特許コード F150008257
掲載日 2015年3月27日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2014JP060185
国際公開番号 WO 2014168143
国際出願日 平成26年4月8日(2014.4.8)
国際公開日 平成26年10月16日(2014.10.16)
優先権データ
  • 特願2013-080738 (2013.4.8) JP
発明の名称 (英語) POLYPEPTIDE FOR FORSTER RESONANCE ENERGY TRANSFER
発明の概要(英語) The purpose of the present invention is to provide a method for measuring the activity of a tyrosine kinase with high efficiency while reducing the number of cells which cannot be evaluated with respect to FRET. The problem can be solved by a polypeptide which is selected from polypeptides (1) and (2) as mentioned below and to which a donor and an acceptor for inducing Forster resonance energy transfer are bound: (1) a polypeptide which comprises an amino acid sequence that has the substitution of at least one amino acid residue selected from amino acid residues lying between position-91 to position-97 and/or at least one amino acid residue selected from amino acid residues lying between position-201 to position-222 in the amino acid sequence represented by SEQ ID NO: 2; and (2) a polypeptide which comprises an amino acid sequence that has the deletion of 1st to 81st amino acid residues as numbered from the carboxyl terminal side and also has the substitution of at least one amino acid residue selected from amino acid residues lying between position-91 to position-97 and/or at least one amino acid residue selected from amino acid residues lying between position-201 to position-222 in the amino acid sequence represented by SEQ ID NO: 2.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • HOKKAIDO UNIVERSITY
  • 発明者(英語)
  • OHBA YUSUKE
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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