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OPTOGENETIC CONTROL METHOD FOR PROLIFERATION AND DIFFERENTIATION OF STEM CELLS

外国特許コード F150008383
掲載日 2015年7月13日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2014JP074458
国際公開番号 WO 2015041219
国際出願日 平成26年9月17日(2014.9.17)
国際公開日 平成27年3月26日(2015.3.26)
優先権データ
  • 特願2013-193582 (2013.9.18) JP
発明の名称 (英語) OPTOGENETIC CONTROL METHOD FOR PROLIFERATION AND DIFFERENTIATION OF STEM CELLS
発明の概要(英語) Using an optogenetic gene expression regulation system, the present invention provides a method for controlling, by using light irradiation to regulate the expression of factors that determine neural differentiation, whether to proliferate and maintain neural precursor cells (NPC), or whether to induce differentiation of neural stem cells into nervous system cells. The present invention is based on: the discovery that neural differentiation factors such as Ascl1, Hes1, and Olig2 are expressed in NPCs periodically in an oscillatory manner but that the expression of any one of these becomes dominant during fate determination; and the structure of gene expression systems in which a target gene can be expressed periodically in an oscillatory manner. In other words, the present invention provides a method for either oscillating the expression of a target factor and maintaining and amplifying NPCs, or inducing sustained expression of the target factor and inducing differentiation into committed nervous system cells by said factor, said method being accomplished by introducing, into NPCs, a nucleic acid which codes a transcriptional activator (GAL4-Vivid-p65 fusion protein; GAVPO) that is activated (dimerized) in response to blue light, and a nucleic acid which links DNA, which codes a target neural differentiation determining factor downstream of a promoter that includes a cis sequence to which the transcriptional activator binds, and a 3’-UTR, which destabilizes mRNA, and by irradiating the NPCs with blue light for short periods of time, changing the cycle.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KYOTO UNIVERSITY
  • 発明者(英語)
  • KAGEYAMA RYOICHIRO
  • ISOMURA AKIHIRO
  • IMAYOSHI ITARU
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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