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SCREENING METHOD, PROTEIN INSTABILITY AND/OR STABILITY INDUCERS, AND PROTEIN ACTIVITY ASSESMENT

外国特許コード F150008446
整理番号 3586
掲載日 2015年10月21日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2013JP065723
国際公開番号 WO 2013183718
国際出願日 平成25年6月6日(2013.6.6)
国際公開日 平成25年12月12日(2013.12.12)
優先権データ
  • 特願2012-129094 (2012.6.6) JP
発明の名称 (英語) SCREENING METHOD, PROTEIN INSTABILITY AND/OR STABILITY INDUCERS, AND PROTEIN ACTIVITY ASSESMENT
発明の概要(英語) Provided is a screening method with which it is possible to determine the cause of a decrease or an increase in the amount of a target protein that is expressed by a cell. One or a plurality of embodiments include: cultivation of a culture including an assay cell brought into contact with a test substance, and measurement of the relative amount of the target protein (A) in relation to an internal standard; cultivation of a culture in which an assay cell is not contacted with a test substance, and measurement of the relative amount (B) in relation to an internal standard; comparison of the relative amount (A) and the relative amount (B); and, based on the comparison, selection of a candidate substance that induces instability and/or stability in the target protein. The assay cell is a cell that is able to express the mRNA of the target protein and the mRNA of the internal standard protein under identical regulation of expression, or a cell having a means for expressing the mRNA of the target protein and the mRNA of the internal standard protein under conditions where the gene expression control is identical.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KYOTO UNIVERSITY
  • NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY
  • 発明者(英語)
  • HAGIWARA MASATOSHI
  • KII ISAO
  • HOSOYA TAKAMITSU
  • SUMIDA YUTO
  • YOSHIDA SUGURU
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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