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METHOD OF PREPARING FUNCTIONALLY ACTIVATED CELL SHEET THROUGH LOW OXYGEN TREATMENT

外国特許コード F160008785
整理番号 (S2014-1298-N0)
掲載日 2016年8月4日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2015JP076207
国際公開番号 WO 2016043201
国際出願日 平成27年9月16日(2015.9.16)
国際公開日 平成28年3月24日(2016.3.24)
優先権データ
  • 特願2014-188322 (2014.9.16) JP
発明の名称 (英語) METHOD OF PREPARING FUNCTIONALLY ACTIVATED CELL SHEET THROUGH LOW OXYGEN TREATMENT
発明の概要(英語) The present invention provides a method of preparing a cell sheet which is more usefully used in the treatment of damage in living tissue. In particular, the present invention provides a method for preparing a cell sheet which achieves a sufficient functional improvement of an infarcted myocardium by transplanting the cell sheet for an infarcted myocardium in myocardial infarction. More specifically, the present invention relates to a method of preparing a cell sheet, the method comprising: a step for culturing cells on a culture substrate to form a cell sheet derived from the cells; a step for culturing the cell sheet under a low oxygen condition at a predetermined temperature for a predetermined period of time; and a step for detaching the cell sheet from the culture substrate after cultured under said condition.
特許請求の範囲(英語) [claim1]
1.  Method for manufacturing a cell sheet comprising the following of (a) ~ (c) step. (A) culturing the cells on a culture substrate, the step of forming the cell sheet from the cells, (B) the cell sheet at a predetermined temperature and low oxygen conditions, the step of a predetermined period of culture, (C) After the culture at the conditions, the process of peeling the cell sheet from the culture substrate
[2]
[claim2]
2.  The method of claim 1 wherein the cell, which is a Cardiosphere derived cells.
[3]
[claim3]
3.  The hypoxic conditions, method according to claim 1 or 2, wherein the oxygen concentration of 0% to 8%.
[4]
[claim4]
4.  A method according to any one of claims 1 to 3 wherein the predetermined temperature, characterized in that it is a 30 ° C ~ 36 ° C.
[5]
[claim5]
5.  The culture period is, the method according to any one of claims 1 to 4, characterized in that the 12 to 72 hours.
[6]
[claim6]
6.  Cell sheet produced by the method according to any one of claims 1 to 5.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • YAMAGUCHI UNIVERSITY
  • 発明者(英語)
  • HAMANO KIMIKAZU
  • HOSOYAMA TOHRU
  • UENO KOJI
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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