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CAR EXPRESSION VECTOR AND CAR-EXPRESSING T CELLS

外国特許コード F160008803
整理番号 (S2014-1580-N0)
掲載日 2016年8月4日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2015JP005080
国際公開番号 WO 2016056228
国際出願日 平成27年10月6日(2015.10.6)
国際公開日 平成28年4月14日(2016.4.14)
優先権データ
  • 特願2014-208200 (2014.10.9) JP
発明の名称 (英語) CAR EXPRESSION VECTOR AND CAR-EXPRESSING T CELLS
発明の概要(英語) The present invention addresses the problem of providing chimeric antigen receptor (CAR)-expressing T cells that express a CAR and also express a T cell immunological function promoting factor, and that have a strong immunity-inducing effect and high antitumor activity. The present invention also addresses the problem of providing a CAR expression vector for producing such CAR-expressing T cells. This CAR expression vector contains a nucleic acid that codes for a CAR, and a nucleic acid that codes for a T cell immunological function promoting factor, wherein the nucleic acid that codes for the T cell immunological function promoting factor comprises either: the nucleic acid that codes for interleukin-7 and the nucleic acid that codes for CCL19; a nucleic acid that codes for a dominant-negative mutant of SHP-1; or a nucleic acid that codes for a dominant-negative mutant of SHP-2. CAR-expressing T cells into which said CAR expression vector has been introduced are produced.
特許請求の範囲(英語) [claim1]
1.

A CAR expression vector containing a nucleic acid encoding immune function promoting factors of nucleic acids and T cells encoding chimeric antigen receptor (CAR), nucleic acid encoding the immune function promoting factor encodes interleukin-7 CAR expression vector characterized in that a nucleic acid encoding a dominant negative mutant for the nucleic acids and nucleic acids encoding CCL19, nucleic acid encoding a dominant negative mutant for SHP-1 or SHP-2,.
[claim2]
2.

Nucleic acids encoding immune function promotion factors, CAR expression vector of claim 1, wherein the a nucleic acid encoding the nucleic acid and CCL19 encoding interleukin 7.
[claim3]
3.

Nucleic acid encoding the immune function promoting factor nucleic acid and T cells encoding the CAR is, CAR expression vector according to claim 2, characterized in that it is linked via a sequence encoding a self-cleaving peptide.
[claim4]
4.

Interleukin nucleic acid encoding the Kin 7 and a nucleic acid encoding the CCL19 is, CAR expression vector of claim 2 or 3, wherein that it is linked via a sequence encoding a self-cleaving peptide.
[claim5]
5.

Nucleic acid encoding the CAR is, CAR expression vector of any one of claims 1 to 4, characterized in that it contains a nucleic acid encoding a polypeptide of FITC or single chain antibodies that recognize CD20.
[claim6]
6.

Nucleic acid encoding the CAR is, CAR expression vector of any one of claims 1 to 5, characterized in that it contains a nucleic acid encoding a polypeptide of the cell transmembrane region of CD8.
[claim7]
7.

Nucleic acid encoding the CAR is, the intracellular region of the-CD28, any one of claims 1 to 6, characterized in that it contains a nucleic acid encoding a polypeptide of the intracellular region, and an intracellular region of CD3ζ of 4-1BB CAR expression vector described.
[claim8]
8.

The following (a) or (b) in CAR expressing T cells transfected with vector shown. (A) CAR expression vector according to any one of claims 1 to 7; (B) CAR expression vectors containing the CAR expression vector containing a nucleic acid encoding the nucleic acid and interleukin 7 encoding CAR, the nucleic acid encoding the nucleic acid and CCL19 encoding CAR;
[claim9]
9.

Anticancer agent containing the CAR expressing T cells and a pharmaceutically acceptable additive according to claim 8.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • YAMAGUCHI UNIVERSITY
  • 発明者(英語)
  • TAMADA KOJI
  • SAKODA YUKIMI
  • ADACHI KEISHI
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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