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NOVEL DIATOM TRANSFORMATION VECTOR AND NOVEL PROMOTER SEQUENCE INCLUDING SAME 新技術説明会

外国特許コード F160008808
整理番号 (S2014-1454-N0)
掲載日 2016年8月4日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2015JP075372
国際公開番号 WO 2016039300
国際出願日 平成27年9月7日(2015.9.7)
国際公開日 平成28年3月17日(2016.3.17)
優先権データ
  • 特願2014-182607 (2014.9.8) JP
発明の名称 (英語) NOVEL DIATOM TRANSFORMATION VECTOR AND NOVEL PROMOTER SEQUENCE INCLUDING SAME 新技術説明会
発明の概要(英語) The invention provides a novel promoter for transforming algae (e.g., diatoms) having a base sequence of SEQ ID NOS: 1-10 or a base sequence having 80% or higher homology with base this sequence, a novel transformation vector including this promoter, and a method for transforming algae using this vector.
特許請求の範囲(英語) [claim1]
1. (1) consisting of SEQ ID NO: 1-10 or a nucleotide sequence polynucleotide; (2) SEQ ID NO: having 80% or more homology to the 1-10 any one of the nucleotide sequences, and polynucleotides function as a promoter results in expression of the gene of interest algae; or (3) SEQ ID NO: 1 or several nucleotides are added to 1 to 10 any one of the nucleotide sequences having deletions and / or substituted nucleotide sequences, and promoter results in expression of the target gene alga polynucleotide that functions as.
[2]
[claim2]
2.  Vector comprising the polynucleotide of claim 1.
[3]
[claim3]
3.  The first expression cassette comprising a polynucleotide and a terminator sequence as set forth in claim 1; and  The polynucleotide of claim 1, the second expression cassette comprising a drug resistance gene, and a terminator sequence; Vector of claim 2 comprising a.
[4]
[claim4]
4.  The vector of claim 2 or 3, wherein the introduction into the host algal cell, method for transforming algae.
[5]
[claim5]
5.  A vector comprising a polynucleotide and a fatty acid hydroxylase gene according to claim 1 is introduced into a host alga cells to obtain transformants, and obtaining a ricinoleic acid by culturing the transformant, ricinoleic production method of acid.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF HYOGO
  • 発明者(英語)
  • IFUKU KENTARO
  • KASHINO YASUHIRO
  • FUKUZAWA HIDEYA
  • KAJIKAWA MASATAKA
  • OGAWA JUN
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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