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TARGET ANALYSIS CHIP AND TARGET ANALYSIS METHOD

外国特許コード F160008847
整理番号 (S2015-0396-N0)
掲載日 2016年9月23日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP051925
国際公開番号 WO 2016117700
国際出願日 平成28年1月22日(2016.1.22)
国際公開日 平成28年7月28日(2016.7.28)
優先権データ
  • 特願2015-010639 (2015.1.22) JP
発明の名称 (英語) TARGET ANALYSIS CHIP AND TARGET ANALYSIS METHOD
発明の概要(英語) The present invention provides a novel target analysis chip and analysis method for directly detecting a target including micro RNAs without performing PCR. The target analysis chip according to the present invention includes a substrate. The substrate includes a reaction part where a sample containing a target is reacted with a reagent, a detection part that is an area where a label is to be detected, and a flow channel that connects the reaction part to the detection part. The reagent contains a carrier, and the carrier has an immobilized label probe to be bound to the target, and forms a bound body with the target. The flow channel includes a movement controlling means for controlling the movement of the carrier from the reaction part to the detection part. The movement controlling means includes a hydrophobic internal wall in the flow channel. When a centrifugal force (C2) larger than a resistance force (R) caused by the hydrophobicity of the flow channel is applied, the movement controlling means enables the bound body to move to the detection part through the flow channel.
特許請求の範囲(英語) [claim1]
1. Possessing baseplate,
The aforementioned baseplate, the reaction section which reacts with the sample and the reagent which include the target, possessing the passage which communicates with the sensing station, and the aforementioned reaction section and the aforementioned sensing station which are the object domain of sign detection,
As for the aforementioned reagent, possessing carrier,
It is something where as for aforementioned carrier, the sign probe which is connected to aforementioned target is fixed, at the same time forms the joining union with the aforementioned target,
The aforementioned passage, possessing the portable control means which control the movement to the aforementioned sensing station from the aforementioned reaction section of the aforementioned carrier,
The aforementioned portable control means, possessing the hydrophobic inner wall in the aforementioned passage,
With the aforementioned portable control means, the aforementioned joining union when the resistivity which it occurs with the hydrophobia of the aforementioned passage (R) centrifugal force it is larger (C2) it is loaded, through the aforementioned passage, features that it can move to the aforementioned sensing station, the target analysis tip/chip.
[claim2]
2. The aforementioned passage, is the structure which strangulates from the aforementioned reaction section side facing toward the aforementioned sensing station side, the analysis tip/chip of claim 1 statement.
[claim3]
3. Height of the aforementioned sensing station, is 1-500 .micro.m, claim the analysis tip/chip of 1 or 2 statements.
[claim4]
4. Capacity of the internal space of the aforementioned sensing station, is smaller than capacity of the internal space of the aforementioned reaction section, from claim 1 either of 3 in one section the analysis tip/chip of statement.
[claim5]
5. The aforementioned carrier, is the beads, from claim 1 either of 5 in one section the analysis tip/chip of statement.
[claim6]
6. Diameter of the aforementioned beads, is 100nm-4 .micro.m, the analysis tip/chip of claim 5 statement.
[claim7]
7. The aforementioned carrier, is the silica make, from claim 1 either of 6 in one section the analysis tip/chip of statement.
[claim8]
8. The side of the aforementioned passage, is the curved surface which becomes narrow in tapered condition from the aforementioned reaction section facing toward the aforementioned sensing station side, from claim 1 either of 7 in one section the analysis tip/chip of statement.
[claim9]
9. The sign of the aforementioned sign probe, the signal is the substance which changes due to the fact that the aforementioned sign probe connects to the aforementioned target, from claim 1 either of 8 in one section the analysis tip/chip of statement.
[claim10]
10. The aforementioned sign, is the substance which includes pyrene, the analysis tip/chip of claim 9 statement.
[claim11]
11. The aforementioned sign probe, furthermore is the probe which is decorated with [soraren], the analysis tip/chip of claim 10 statement.
[claim12]
12. The aforementioned target, is micro RNA, from claim 1 either of 11 in one section the analysis tip/chip of statement.
[claim13]
13. The quantity of the aforementioned sign probe, is 1zmol-1nmol, from claim 1 either of 12 in one section the analysis tip/chip of statement.
[claim14]
14. The aforementioned reagent, furthermore includes the surface active agent, from claim 1 either of 13 in one section the analysis tip/chip of statement.
[claim15]
15. The aforementioned reaction section, combines the sample introduction section where the aforementioned sample is introduced, from claim 1 either of 14 in one section the analysis tip/chip of statement.
[claim16]
16. The aforementioned baseplate, furthermore implication the passage which communicates with the sample introduction section and the aforementioned sample introduction section and the aforementioned reaction section where the aforementioned sample is introduced,
It can move the aforementioned sample in the aforementioned sample introduction section, to the aforementioned reaction section the aforementioned centrifugal force (C2) centrifugal force under (C1) due to, from claim 1 either of 15 in one section the analysis tip/chip of statement.
[claim17]
17. The passage which communicates with the aforementioned sample introduction section and the aforementioned reaction section, furthermore has the filter, the analysis tip/chip of claim 16 statement.
[claim18]
18. From claim 1 either of 17 for one section using target analysis tip/chip of statement,
In the aforementioned reaction section, the process which introduces the sample and the reagent,
In the aforementioned reaction section, the process which makes with the aforementioned sample and the carrier contact where the aforementioned sign probe is fixed, forms the joining union with the aforementioned target and the aforementioned sign probe by connecting reacts the target and the aforementioned sign probe in the aforementioned sample,
The process which is made to move to the aforementioned sensing station the resistivity which it occurs the joining union in the aforementioned reaction section, with the hydrophobia of the inner wall of the aforementioned passage (R) centrifugal force it is larger (C2) due to, and,
It features that the process which analyzes the target in the aforementioned sample in the aforementioned sensing station, by detecting the sign of the aforementioned sign probe which the aforementioned target connects, is included, target analysis method.
[claim19]
19. In the aforementioned reaction section, the blend which includes with the aforementioned sample and the aforementioned carrier is agitated, analysis method of claim 18 statement.
[claim20]
20. The sign of the aforementioned sign probe, the signal is the substance which changes due to the fact that the aforementioned sign probe connects to the aforementioned target,
In the aforementioned sensing station, signal change of the aforementioned sign is detected, claim 18 or analysis method of 19 statements.
[claim21]
21. The aforementioned sign, is the substance which includes pyrene,
Ultraviolet light is irradiated to the aforementioned sensing station, the fluorescent signal of wave length 450-510nm is detected, analysis method of claim 20 statement.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • ARKRAY, INC.
  • Kyoto Institute of Technology
  • 発明者(英語)
  • MURAKAMI AKIRA
  • KOBORI AKIO
  • YAMAYOSHI ASAKO
  • NODA YUICHIRO
  • KONDO MASAYUKI
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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