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METHOD FOR PREPARING BONE MARROW CELL AGGREGATE

外国特許コード F160008849
整理番号 (S2015-0410-N0)
掲載日 2016年9月23日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP051009
国際公開番号 WO 2016121512
国際出願日 平成28年1月14日(2016.1.14)
国際公開日 平成28年8月4日(2016.8.4)
優先権データ
  • 特願2015-014194 (2015.1.28) JP
発明の名称 (英語) METHOD FOR PREPARING BONE MARROW CELL AGGREGATE
発明の概要(英語) Provided is a technique whereby bone marrow cells can be organized by a simple method within a short period of time. A method for preparing a bone marrow cell aggregate, said method comprising adding a bone marrow cell mass-containing liquid to a medium that contains a swellable material, and culturing the bone marrow cell mass in the presence of the swellable material. A method for reconstructing a bone marrow tissue, said method comprising adding a bone marrow cell mass-containing liquid to a medium that contains a swellable material, and culturing the bone marrow cell mass in the presence of the swellable material. As the common sense in the art, it has been believed that, after disintegrating a bone marrow tissue, cell masses can be hardly reorganized while keeping the cell composition unchanged, i.e., without adding "a binder" such as adhesive cells or intracellular matrix. In fact, such cell masses cannot be aggregated by methods generally employed in the art. The present invention provides a remarkable breakthrough technique relating to the three-dimensional culture of bone marrow cells to thereby overcome the above problem and situation encountered in the prior art. It is also confirmed that a bone marrow-like tissue reconstructed by the method according to the present invention can be continuously cultured in a methyl celluose-containing medium until the 14th day.
特許請求の範囲(英語) [claim1]
1. The marrow cell population content liquid is added to the nutrient medium which contains the swelling material, the fact that the marrow cell population is cultured under existing of the swelling material is included, production method of the marrow cell cohesion body.
[claim2]
2. The marrow cell population content liquid is added to the nutrient medium which contains the swelling material, the fact that the marrow cell population is cultured under existing of the swelling material is included, the method marrow organization being re-constructed.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • YOKOHAMA CITY UNIVERSITY
  • 発明者(英語)
  • KOJIMA NOBUHIKO
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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