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TECHNIQUE FOR AGGREGATING MACROMOLECULES TOGETHER WITH CELLS

外国特許コード F170008919
整理番号 (S2015-1519-N0)
掲載日 2017年1月19日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP064167
国際公開番号 WO 2016182022
国際出願日 平成28年5月12日(2016.5.12)
国際公開日 平成28年11月17日(2016.11.17)
優先権データ
  • 特願2015-099064 (2015.5.14) JP
発明の名称 (英語) TECHNIQUE FOR AGGREGATING MACROMOLECULES TOGETHER WITH CELLS
発明の概要(英語) Provided is a technique for efficiently aggregating macromolecules such as ECM together with cells. A method for creating a cell or a cell aggregate loaded with macromolecules, the method comprising adding, to a medium containing a swellable material, a solution containing macromolecules and at least one cell to aggregate the macromolecules together with the cell. A method for controlling the function and/or the property of a cell or a cell aggregate, the method comprising culturing the cell or the cell aggregate loaded with the macromolecules created in the aforementioned method. A method for culturing a cell or a cell aggregate, the method comprising: adding, to a medium containing a swellable material, a solution containing macromolecules and at least one cell to aggregate the macromolecules together with the cell and to produce a macromolecule capsule encapsulating the cell or the cell aggregate; and culturing the cell or the cell aggregate in the capsule. For preparing a three-dimensional cell tissue, methods such as 96-well U-bottom plating and hanging drop technique are conventionally used. In the methods of the present invention, 2000 cells are mixed with 1 μl of the medium containing ECM, and the ECM and cells can be simultaneously aggregated. If the efficiency here is defined as 1, a calculation can be made that, when 96-well U-bottom plating or hanging drop technique is used, ECM can only be mixed in the three-dimensional cell tissue at an efficiency of about 1/12000 or about 1/2400, respectively. Thus, with the present invention, the cost for ECM can be reduced to about one several-thousandth to one hundred-thousandth, and it becomes possible to effectively use artificial ECM that cannot be synthesized in large quantity or rare ECM that can be extracted only at a very small amount because of reasons such as the subject being a small living organism.
特許請求の範囲(英語) [claim1]
1. The macromolecule and adding the solution which at least includes with 1 cells to the nutrient medium which contains the swelling material, it includes the fact that it coheres the macromolecule and the cell together, the method of producing the cell or cell cohesion body where the macromolecule fills up.
[claim2]
2. The cell cohesion body, method of the claim 1 statement which forms 3 dimensional organization.
[claim3]
3. Characteristics of 3 dimensional organization and/or function, characteristics of the cell which does not form 3 dimensional organization and/or on the functional or intercellular without filling up the macromolecule characteristics of 3 dimensional organization which was formed and/or from function method of the claim 2 statement which has improved.
[claim4]
4. The cell or cell cohesion body where the macromolecule fills up, in either of the claim 1-3 which is the high-molecular capsule which encloses the cell or cell cohesion body method of statement.
[claim5]
5. In either of the claim 1-4 which is the cell or cell cohesion body where the macromolecule which 1 where it is included in the solution which it adds to the nutrient medium containing the swelling material cells, in either of the claim 1-4 produce at least with method of statement fills up method of statement.
[claim6]
6. In either of the claim 1-5 it produced with method of statement, the fact that the cell or cell cohesion body where the macromolecule fills up is cultured is included, characteristics of the cell or cell cohesion body and or the method of controlling function.
[claim7]
7. The macromolecule and adding the solution which at least includes with 1 cells to the nutrient medium which contains the swelling material, by the fact that it coheres the macromolecule and the cell together, it produces the high-molecular capsule which encloses the cell or cell cohesion body, it includes the fact that the cell or cell cohesion body is cultured inside this capsule, culture method of the cell or cell cohesion body.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • YOKOHAMA CITY UNIVERSITY
  • 発明者(英語)
  • KOJIMA NOBUHIKO
国際特許分類(IPC)
指定国 (WO2016182022)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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