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METHOD FOR PRODUCING TRANSFORMED CELL

外国特許コード F170008938
整理番号 (S2015-0632-N0)
掲載日 2017年1月20日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP061420
国際公開番号 WO 2016163466
国際出願日 平成28年4月7日(2016.4.7)
国際公開日 平成28年10月13日(2016.10.13)
優先権データ
  • 特願2015-078489 (2015.4.7) JP
発明の名称 (英語) METHOD FOR PRODUCING TRANSFORMED CELL
発明の概要(英語) The purpose of the present invention is to provide an easy and high-throughput method whereby a DNA can be transferred between different hosts. To achieve this purpose, provided is a method for producing a transformed cell of a yeast or Escherichia coli , said method being characterized in that the yeast or Escherichia coli is transformed using a vector-containing liquid which contains (A) a lytic solution containing a Bacillus subtilis lysate and a vector released therefrom and (B) additive(s) selected from the group consisting of a nuclease inhibitor, a protein-denaturing agent, a nuclease and a combination of two or more of the same.
特許請求の範囲(英語) [claim1]
1. (A) the Bacillus subtilis which the bacteriolysis is done and the bacteriolysis liquid which includes the vector which then is discharged, and
(B) the nuclease inhibiter and the protein denaturant, the additive which is selected from the group which consists of the combination above the nuclease, and those two,
It features that character it converts the yeast or the colon bacillus making use of the vector content liquid which is included, production method of the transformation cell.
[claim2]
2. The aforementioned nuclease inhibiter, [aurintorikarubon] and the surface active agent, the chelating agent, in the claim 1 which is reducing agent, [banijirunukureochido], hydrogen peroxide, or the anti- nuclease antibody production method of the transformation cell of statement.
[claim3]
3. The aforementioned protein denaturant is the proteolytic enzyme or the surface active agent, in claim 1 or 2 production method of the transformation cell of statement.
[claim4]
4. The aforementioned nuclease, [ekisonukureaze] I, [ekisonukureaze] III, is the [mangubinnukureaze], or S1 nuclease, either of the claim 1-3 in one section production method of the transformation cell of statement.
[claim5]
5. The aforementioned transformation, is done the vector content liquid and mixture or the vector content liquid and by the yeast or the colon bacillus with the yeast or colon bacillus competent cell [erekutoroporeshiyon] to the mixed liquid, either of the claim 1-4 in one section production method of the transformation cell of statement.
[claim6]
6. The aforementioned bacteriolysis, is done the quiet position of the Bacillus subtilis culture solution, by the infection of the bacteriophage, the addition of the surface active agent or the combination of those two or more, either of the claim 1-5 in one section production method of the transformation cell of statement.
[claim7]
7. Either of the claim 1-6 in one section the transformation cell which can by the production method of statement.
[claim8]
8. Either of the claim 1-6 in one section the library which can by the production method of statement.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOKYO INSTITUTE OF TECHNOLOGY
  • KEIO UNIVERSITY
  • 発明者(英語)
  • KANEKO SHINYA
  • ITAYA MITSUHIRO
国際特許分類(IPC)
指定国 (WO2016163466)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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