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METHOD FOR PRODUCING CANINE iPS CELLS

外国特許コード F170008953
整理番号 (S2015-1659-N0)
掲載日 2017年2月16日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP068194
国際公開番号 WO 2016204298
国際出願日 平成28年6月19日(2016.6.19)
国際公開日 平成28年12月22日(2016.12.22)
優先権データ
  • 特願2015-123775 (2015.6.19) JP
発明の名称 (英語) METHOD FOR PRODUCING CANINE iPS CELLS
発明の概要(英語) [Problem] To produce, by using a liquid culture having an extremely simple composition, a canine iPS cell line that can be subcultured stably for a long period of time. [Solution] The present invention obtains canine iPS cells by culturing canine somatic cells having introduced therein four transcription factors of OCT3/4, SOX2, KLF4, and C-MYC that are nuclear reprogramming factors by means of a drug-inducible vector that can control expression of the transcription factors through usage of, for example, doxycycline, in a liquid culture containing basic fibroblast growth factor (bFGF) but not containing a pluripotency maintaining factor (not including basic fibroblast growth factor) such as LIF, and further preferably not containing a differentiation suppressor such as MEK suppressor and GSK3β suppressor.
特許請求の範囲(英語) [claim1]
1. Being the method of producing dog iPS cell stocks from the somatic cell of the dog,
The process which introduces nuclear initialization factor into the dog somatic cell and,
The method of including the process which cultures the dog somatic cell which introduces nuclear initialization factor, differentiation versatile characteristic maintenance factor making use of the culture solution which is not included under existing of basic tissue bud cell growth factor.
[claim2]
2. As for the aforementioned culture solution, differentiation inhibiter in the claim 1 which is the culture solution which is not included method of statement.
[claim3]
3. As for the aforementioned culture solution, in the claim 1 which designates the N2B27 nutrient medium as the basal medium or 2 method of statement.
[claim4]
4. Being the method of producing dog iPS cell stocks from the somatic cell of the dog,
The process which introduces nuclear initialization factor into the dog somatic cell and,
The method of including the process which cultures the dog somatic cell which introduces nuclear initialization factor, making use of the N2B27 nutrient medium which includes only basic tissue bud cell growth factor.
[claim5]
5. As for the aforementioned nuclear initialization factor the next three genes: Either of the claim 1-4 which is OCT3/4, SOX2 and KLF4, or is four genes which furthermore include C-MYC in the particular three genes in 1 sections method of statement.
[claim6]
6. Either of the claim 1-5 which introduces the aforementioned nuclear initialization factor into the dog somatic cell making use of the medicine inductive vector which can be controlled revelation of the aforementioned nuclear initialization factor, with the addition of the medicine, in 1 sections method of statement.
[claim7]
7. Either of the claim 1-6 which cultures the dog somatic cell where on [matorigeru] which under existing of the feeder cell or under non existing of the feeder cell is, the aforementioned nuclear initialization factor is introduced in 1 sections method of statement.
[claim8]
8. Either of the claim 1-7 the dog iPS cell which was drawn up with the method of statement to 1 sections.
[claim9]
9. The process which introduces nuclear initialization factor into the dog somatic cell and,
The dog somatic cell which introduces nuclear initialization factor, culturing differentiation versatile characteristic maintenance factor under existing of basic tissue bud cell growth factor making use of the culture solution which is not included, the process which obtains dog iPS cell stocks and,
The process which it differentiates induces the cell which is obtained with culture is included, production method of the dog somatic cell.
[claim10]
10. As for the aforementioned dog somatic cell, in the claim 9 which is the mesenchyme type trunk cell production method of the dog somatic cell of statement.
[claim11]
11. As for the aforementioned dog somatic cell, in the claim 9 which is the bone bud cell or the nervous trunk cell production method of the dog somatic cell of statement.
[claim12]
12. The cell of the dog somatic cell origin which is separated from the organism where at least one gene of endogenous OCT3/4, KLF4 and SOX2, desirably, you can see the revelation of all genes and the NANOG gene at the same time possesses differentiation versatile characteristic.
[claim13]
13. Furthermore, in the claim 12 where you can see the revelation of the gene of GBX2 the cell of dog somatic cell origin of statement.
[claim14]
14. Under existing of [dokishisaikurin], at least the one of OCT3/4, KLF4 and SOX2 of the exogenous gene, desirably, when revelation of all genes, furthermore C-MYC is introduced, in the claim 12 where you can see the revelation of these four genes or 13 the cell of statement.
[claim15]
15. With enzyme processing in the single cell division either of the claim 12-14 which is possible in 1 sections the cell of statement.
[claim16]
16. Either of the claim 12-15 the dog somatic cell which in 1 sections it was differentiated was induced from the cell of statement.
[claim17]
17. Either of the claim 12-15 the mesenchyme type trunk cell which in 1 sections it was differentiated was induced from the cell of statement.
[claim18]
18. Either of the claim 12-15 the bone bud cell or the nervous trunk cell which in 1 sections it was differentiated was induced from the cell of statement.
[claim19]
19. Either of the claim 12-15 differentiating inducing the cell of statement in 1 sections, the method of producing the dog somatic cell.
[claim20]
20. Either of the claim 12-15 differentiating inducing the cell of statement in 1 sections, the method of producing the mesenchyme type trunk cell.
[claim21]
21. Either of the claim 12-15 differentiating inducing the cell of statement in 1 manuscripts, the method of producing the bone bud cell or the nervous trunk cell.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • OSAKA PREFECTURE UNIVERSITY
  • 発明者(英語)
  • INABA TOSHIO
  • NISHIMURA TOSHIYA
  • TANAKA ERINA
  • HATOYA SHINGO
  • SUGIURA KIKUYA
国際特許分類(IPC)
指定国 (WO2016204298)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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