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HIGH-DENSITY MICRO-CHAMBER ARRAY AND MEASUREMENT METHOD USING SAME NEW

外国特許コード F170008976
整理番号 K10406WO
掲載日 2017年3月13日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP066834
国際公開番号 WO 2016199741
国際出願日 平成28年6月7日(2016.6.7)
国際公開日 平成28年12月15日(2016.12.15)
優先権データ
  • 特願2015-116045 (2015.6.8) JP
発明の名称 (英語) HIGH-DENSITY MICRO-CHAMBER ARRAY AND MEASUREMENT METHOD USING SAME NEW
発明の概要(英語) The present invention comprises: a flat substrate having a light-transmitting property; a hydrophobic layer in which a plurality of micro chambers are provided; and a lipid bilayer that is formed in openings of the plurality of micro chambers. An electrode is provided inside each of the micro chambers. The substrate is configured such that, as a result of at least either of the following A) and B) being satisfied when the side of the substrate on which the hydrophobic layer is provided is regarded as the upper side, light incident to the substrate from below the substrate passes through the substrate and enters the insides of the micro chambers and light incident to the substrate from the insides of the micro chambers passes through the substrate and escapes to below the substrate. A) An electrode is provided on an inner lateral surface of each micro chamber. B) An electrode is provided as a transparent electrode on a bottom surface of each micro chamber.
特許請求の範囲(英語) [claim1]
1. The even baseplate which possesses the translucency and,
Being the layer which is provided on the aforementioned baseplate and consists of the hydrophobic substance, in order for the open part of the plural minute chambers to arrange into systematic and high density on the principal plane of the said layer, capacity of the providing and the aforementioned minute chamber the 4000x10 (- 18) is m (3) or less, hydrophobic layer and,
In order to seal the liquid for the aforementioned test in the open part of the aforementioned plural minute chambers of a state where the liquid for test has been filled up, the lipid double membrane which was formed having,
The electrode to be provided inside the respective aforementioned minute chamber,
In the aforementioned baseplate, when designating the side where the aforementioned hydrophobic layer is provided as the upper part,
Description below A) And B) From the lower part of the aforementioned baseplate the light which incidence is done transmitting the aforementioned baseplate to the aforementioned baseplate at least in each case by satisfying one side, it penetrates to inside the aforementioned minute chamber, at the same time, from inside the aforementioned minute chamber the light which incidence is done transmitting the aforementioned baseplate to the aforementioned baseplate, in order to escape to the lower part of the aforementioned baseplate, it is constituted,
High density minute chamber array.
A) The aforementioned electrode, is provided on the inside surface of the respective aforementioned minute chamber.
B) The aforementioned electrode, is provided in the base of the respective aforementioned minute chamber as the transparent electrode.
[claim2]
2. The aforementioned electrode being the metal, it is provided on the inside surface of the respective aforementioned minute chamber, in claim 1 high density minute chamber array of statement.
[claim3]
3. The aforementioned metal is the chrome, in claim 2 high density minute chamber array of statement.
[claim4]
4. The biopolymer is accumulated inside the aforementioned minute chamber, claim in either 1 or 3 high density minute chamber array of statement.
[claim5]
5. Furthermore, it has the opposite electrode to the upper part of the aforementioned lipid double membrane, claim in either 1 or 4 high density minute chamber array of statement.
[claim6]
6. It has the liquid passage where the surface where the aforementioned minute chamber was formed becomes the base,
Claim in either 1 or 5 high density minute chamber array of statement.
[claim7]
7. In claim 5 high density minute chamber array of statement and,
It has with the voltage impression device which impresses voltage with the aforementioned electrode and the aforementioned opposite electrode,
High density minute chamber array system.
[claim8]
8. In claim 2 or 3 high density minute chamber array of statement and,
Inside the aforementioned electrode it has with the electric current impression device which becomes feverish the aforementioned electrode by the fact that electric current is let flow parallel with the aforementioned baseplate,
High density minute chamber array system.
[claim9]
9. Furthermore, the opposite electrode which is provided in the upper part of the aforementioned lipid double membrane and,
It has with the voltage impression device which impresses voltage with the aforementioned electrode and the aforementioned opposite electrode,
In claim 8 high density minute chamber array system of statement.
[claim10]
10. Being the layer which consists of the hydrophobic substance which is provided on the even baseplate and the aforementioned baseplate which possess the translucency, in order for the open part of the plural minute chambers to arrange into systematic and high density on the principal plane of the said layer, capacity of the providing and the aforementioned minute chamber the 4000x10 (- 18) is m (3) or less, it has with hydrophobic layer, the electrode is provided inside the respective aforementioned minute chamber, when designating the side where the aforementioned hydrophobic layer is provided in the aforementioned baseplate, as the upper part, description below A) and B) at least in each case by satisfying one side, the lower part of the aforementioned baseplateFrom the light which incidence is done transmitting the aforementioned baseplate to the aforementioned baseplate, it penetrates to inside the aforementioned minute chamber, at the same time, from inside the aforementioned minute chamber the light which incidence is done transmitting the aforementioned baseplate to the aforementioned baseplate, in order to escape to the lower part of the aforementioned baseplate, it is constituted, high density minute chamber array is prepared,
The lipid double membrane is formed in the open part of the aforementioned plural minute chambers, the aforementioned lipid double membrane is something which keeps the membrane protein here,
It changes the character of aforementioned membrane protein by the fact that voltage is impressed with the aforementioned electrode and the opposite electrode which is provided in the upper part of the aforementioned lipid double membrane,
Analytical method of membrane protein.
A) The aforementioned electrode, is provided on the inside surface of the respective aforementioned minute chamber.
B) The aforementioned electrode, is provided in the base of the respective aforementioned minute chamber as the transparent electrode.
[claim11]
11. Being the layer which consists of the hydrophobic substance which is provided on the even baseplate and the aforementioned baseplate which possess the translucency, in order for the open part of the plural minute chambers to arrange into systematic and high density on the principal plane of the said layer, capacity of the providing and the aforementioned minute chamber the 4000x10 (- 18) is m (3) or less, it has with hydrophobic layer, the electrode is provided inside the respective aforementioned minute chamber, when designating the side where the aforementioned hydrophobic layer is provided in the aforementioned baseplate, as the upper part, description below A) and B) at least in each case by satisfying one side, the lower part of the aforementioned baseplateFrom the light which incidence is done transmitting the aforementioned baseplate to the aforementioned baseplate, it penetrates to inside the aforementioned minute chamber, at the same time, from inside the aforementioned minute chamber the light which incidence is done transmitting the aforementioned baseplate to the aforementioned baseplate, in order to escape to the lower part of the aforementioned baseplate, it is constituted, high density minute chamber array is prepared,
By the fact that voltage is impressed in the aforementioned electrode, to accumulate the biopolymer inside aforementioned plural minute chambers, after that,
In order to seal the aforementioned biopolymer in the open part of the aforementioned plural minute chambers, the lipid double membrane is formed, method.
A) The aforementioned electrode, is provided on the inside surface of the respective aforementioned minute chamber.
B) The aforementioned electrode, is provided in the base of the respective aforementioned minute chamber as the transparent electrode.
[claim12]
12. High density minute chamber array of statement is prepared in claim 2 or 3,
Temperature of the liquid for the aforementioned test which is sealed inside the aforementioned minute chamber by the fact that it becomes feverish the aforementioned electrode by the fact that electric current is let flow to the aforementioned electrode, is controlled, method.
[claim13]
13. High density minute chamber array of statement is prepared in claim 5,
By the fact that the cell is made to fuse to aforementioned lipid double membrane by impressing electric current with the aforementioned electrode and the aforementioned opposite electrode, it moves the membrane protein of the aforementioned cell origin to the aforementioned lipid double membrane, method.
[claim14]
14. As for the step which prepares the aforementioned high density minute chamber array, implication the step which provides the opposite electrode to the upper part of the aforementioned lipid double membrane,
The aforementioned membrane protein was introduced to the aforementioned lipid double membrane by the fact that the cell is made to fuse to aforementioned lipid double membrane by impressing electric current with the aforementioned electrode and the aforementioned opposite electrode, it is the membrane protein of the aforementioned cell origin, in claim 10 analytical method of the membrane protein of statement.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • WATANABE RIKIYA
  • NOJI HIROYUKI
  • SOGA NAOKI
国際特許分類(IPC)
指定国 (WO2016199741)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
参考情報 (研究プロジェクト等) PRESTO Structural life science and advanced core technologies for innovative life science research AREA
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