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DNA FOR INTRACELLULAR PENETRATION, AND METHOD FOR INTRODUCING TARGET MOLECULE INTO CELL USING SAID DNA 新技術説明会

外国特許コード F170009018
整理番号 (S2015-2057-N0)
掲載日 2017年3月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP077449
国際公開番号 WO 2017047750
国際出願日 平成28年9月16日(2016.9.16)
国際公開日 平成29年3月23日(2017.3.23)
優先権データ
  • 特願2015-185295 (2015.9.18) JP
発明の名称 (英語) DNA FOR INTRACELLULAR PENETRATION, AND METHOD FOR INTRODUCING TARGET MOLECULE INTO CELL USING SAID DNA 新技術説明会
発明の概要(英語) The present invention provides: DNA for intracellular penetration; a composition that includes said DNA and a target molecule to be introduced into a cell; a method for introducing the target molecule into the cell using said composition; and an agent and kit for introducing the target molecule into the cell, said agent and kit including said DNA. Provided is DNA for intracellular penetration, said DNA including an intracellular penetration region comprising the following base sequence: 5'-N1-N2-GG-N5-N6-N7-GGTGGT-N14-GGGG-N19-N20-G-N22-N23-TCG-N27-N28-N29-G-N31-AT-N34-N35-GTG-N39-N40-TCG-3' [Herein, N1 is deleted or is T, N2 is G or is deleted, N5 is C or T, N6 is G or C, N7 is G or T, N14 is G or C, N19 is A or G, N20 is G or A, N22 is deleted or is T, N23 is T or A, N27 is G, T, or A, N28 is T, A, or C, N29 is deleted or is T, N31 is G or A, N34 is G or is deleted, N35 is C or A, N39 is G or T, and N40 is G or A] (SEQ ID NO: 1).
特許請求の範囲(英語) [claim1]
1. Base arrangement below:
5 ' - N1-N2-GG-N5-N6-N7-GGTGGT-N14-GGGG-N19-N20-G-N22-N23-TCG-N27-N28-N29-G-N31-AT-N34-N35-GTG-N39-N40-TCG-3'
[Here,
N1 has been deficient or or it is T,
N2 is G or or we have been deficient,
N5 C or is T,
N6 G or is C,
N7 is G or T,
N14 G or is C,
N19 is A or G,
N20 is G or A,
N22 has been deficient or or it is T,
N23 is T or A,
N27, is G, T or A,
N28, T and A or is C,
N29 has been deficient or or it is T,
N31 is G or A,
N34 is G or or we have been deficient,
N35 C or is A,
N39 to be G or T, at the same time
N40 is G or A] (arrangement number 1)
The cell inner movement territory which consists of is included, movement characteristic DNA inside the cell.
[claim2]
2. The aforementioned base arrangement is, (a) below or (b), in claim 1 movement characteristic DNA inside the cell of statement.
(A) 5 ' - GGGCGGGGTGGTGGGGGAGGTTCGGTGGATGCGTGGGTCG-3' (arrangement number 2),
5 ' - GGTGGGGTGGTGGGGGAGGTTCGTTGGATGCGTGGGTCG-3' (arrangement number 3),
5 ' - GGGCGGGGTGGTCGGGGGAGTTTCGGTGGATGCGTGGGTCG-3' (arrangement number 4),
5 ' - GGGCGGGGTGGTGGGGGAGGTTCGTATGGATGCGTGGATCG-3' (arrangement number 5),
5 ' - GGGCGGGGTGGTGGGGGAGGTTCGATGGATCGTGGGTCG-3' (arrangement number 6),
5 ' - GGGCGGGGTGGTGGGGGAGGATCGGTGGATGCGTGGGTCG-3' (arrangement number 7),
5 ' - GGCGGGGTGGTGGGGGAGGTTCGATGGATGAGTGGGTCG-3' (arrangement number 8),
5 ' - GGGCCGGGTGGTGGGGGAGGTTCGGTGGATGCGTGGGTCG-3' (arrangement number 9),
5 ' - GGGCGGGGTGGTGGGGGAGGTTCGACGGATGCGTGGGTCG-3' (arrangement number 10),
5 ' - TGGGCGGGGTGGTGGGGGAGGTTCGGTGAATGCGTGTGTCG-3' (arrangement number 11), and
5 ' - GGGCGTGGTGGTGGGGGAGGTTCGGTGGATGCGTGGGTCG-3' (arrangement number 12)
The base arrangement which is shown to in each case
(B) The base arrangement which 1 or 2 bases substitute at the time of base arranging which is shown in either of the arrangement number 2-12, deficiency, are inserted or are added
[claim3]
3. Base arrangement below:
5 ' - N1-N2-GG-N5-N6-N7-GGTGGT-N14-GGGG-N19-N20-G-3'
[Here,
N1 has been deficient or or it is T,
N2 is G or or we have been deficient,
N5 C or is T,
N6 G or is C,
N7 is G or T,
N14 G or is C,
N19 to be A or G, at the same time
N20 is G or A] (arrangement number 13)
The cell inner movement territory which 2 or more is included is included, movement characteristic DNA inside the cell.
[claim4]
4. Each one of the aforementioned base arrangement is, (a) below or (b), in claim 3 movement characteristic DNA inside the cell of statement.
(A) 5 ' - GGGCGGGGTGGTGGGGGAGG-3' (arrangement number 14),
5 ' - GGTGGGGTGGTGGGGGAGG-3' (arrangement number 15),
5 ' - GGGCGGGGTGGTCGGGGGAG-3' (arrangement number 16),
5 ' - GGCGGGGTGGTGGGGGAGG-3' (arrangement number 17),
5 ' - GGGCCGGGTGGTGGGGGAGG-3' (arrangement number 18),
5 ' - TGGGCGGGGTGGTGGGGGAGG-3' (arrangement number 19), and
5 ' - GGGCGTGGTGGTGGGGGAGG-3' (arrangement number 20)
The base arrangement which is shown to in each case
(B) The base arrangement which 1 bases substitute at the time of base arranging which is shown in either of the arrangement number 14-20, deficiency, are inserted or are added
[claim5]
5. The movement characteristic DNA inside the cell 2 or more connecting, it becomes, being the polymer of movement characteristic DNA inside the cell, each one of movement characteristic DNA inside the said cell either of the claim 1-4 from movement characteristic DNA inside the cell of statement is selected to one section, the movement characteristic DNA polymer inside the cell.
[claim6]
6. Either of the claim 1-4 movement characteristic the movement characteristic DNA polymer inside the cell of statement is included in DNA inside the cell of statement or claim 5 in one section, the introduction medicine inside the cell of the intended molecule.
[claim7]
7. The intended molecule which either of the claim 1-4 in one section movement characteristic with DNA inside the cell of statement or the movement characteristic DNA polymer inside the cell of statement and it tries to introduce into the cell in claim 5 it includes, the composition.
[claim8]
8. Being the method of introducing the intended molecule into the cell, it includes the fact that it makes the composition of statement contact with the cell in claim 7, method.
[claim9]
9. Either of the claim 1-4 movement characteristic DNA inside the cell of statement or the movement characteristic DNA polymer inside the cell of statement is included in claim 5 in one section, the kit for introduction inside cell for the intended molecule.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • OSAKA CITY UNIVERSITY
  • 発明者(英語)
  • TACHIBANA AKIRA
  • TANABE TOSHIZUMI
国際特許分類(IPC)
指定国 (WO201747750)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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