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Reagent for imaging intracellular acetylation

外国特許コード F170009042
整理番号 E112P01WO
掲載日 2017年4月26日
出願国 アメリカ合衆国
出願番号 201314434270
公報番号 20150276751
出願日 平成25年10月10日(2013.10.10)
公報発行日 平成27年10月1日(2015.10.1)
国際出願番号 JP2013077542
国際公開番号 WO2014057999
国際出願日 平成25年10月10日(2013.10.10)
国際公開日 平成26年4月17日(2014.4.17)
優先権データ
  • 特願2012-225841 (2012.10.11) JP
  • 2013WO-JP77542 (2013.10.10) WO
発明の名称 (英語) Reagent for imaging intracellular acetylation
発明の概要(英語) (US20150276751)
A reagent for imaging an advancing intracellular acetylation, for example, an acetylation that is advanced by the action of acetyl-CoA in the mitochondria, at high sensitivity and high efficiency, which comprises a combination of a rhodamine derivative that is substantially non-fluorescent before being acetylated, and emits strong fluorescence after being acetylated, and an acylation catalyst and/or an acylation reaction-promoting agent.
特許請求の範囲(英語) [claim1]
1. A reagent for visualizing an intracellular acetylation, which comprises a combination of a rhodamine derivative that is substantially non-fluorescent before being acetylated, and emits strong fluorescence after being acetylated, and an acylation catalyst and/or an acylation reaction-promoting agent.
[claim2]
2. The reagent according to claim 1, wherein the intracellular acetylation is an acetylation advanced by acetyl-CoA.
[claim3]
3. A reagent for measuring intracellular acetyl-CoA, which comprises a combination of a rhodamine derivative that is substantially non-fluorescent before being acetylated, and emits strong fluorescence after being acetylated, and an acylation catalyst and/or an acylation reaction-promoting agent.
[claim4]
4. The reagent according to claim 1, wherein the rhodamine derivative is a rhodamine derivative represented by the following general formula (I):
wherein, in the formula, R1 represents hydrogen atom or a C1-6 alkyl group; R2 represents hydrogen atom, a C1-6 alkyl group, or carboxyl group; R3 and R5 independently represent hydrogen atom, a halogen atom, or a C1-6 alkyl group; R4 and R6 independently represent hydrogen atom or a C1-6 alkyl group; R7 and R8 independently represent a C1-6 alkyl group, but the alkyl group represented by R7 and the alkyl group represented by R3 may bind together to form a 5- to 7-membered ring, and/or the alkyl group represented by R8 and the alkyl group represented by R4 may bind together to form a 5- to 7-membered ring; R9 and R10 independently represent a C1-6 alkyl group, but the alkyl group represented by R9 and the alkyl group represented by R5 may bind together to form a 5- to 7-membered ring, and/or the alkyl group represented by R10 and the alkyl group represented by R6 may bind together to form a 5- to 7-membered ring; X- represents a counter ion; and Y represents oxygen atom or M(R11)(R12) (M represents silicon atom, germanium atom, or tin atom; and R11 and R12 independently represents a C1-6 alkyl group).
[claim5]
5. The reagent according to claim 4, wherein R1 is hydrogen atom or a C1-6 alkyl group; R2 is hydrogen atom, a C1-6 alkyl group, or carboxyl group; R3 and R5 are hydrogen atoms; R4 and R6 are hydrogen atoms; R7 and R8 are independently C1-6 alkyl groups; R9 and R10 are independently C1-6 alkyl groups; X- is a counter ion; and Y is oxygen atom.
[claim6]
6. The reagent according to claim 4, wherein R1 is hydrogen atom; R2 is hydrogen atom; R3, R4, R5, and R6 are hydrogen atoms; R7, R8, R9, and R10 are methyl groups; X- is a counter ion; and Y is oxygen atom.
[claim7]
7. The reagent according to claim 1, wherein the acylation catalyst or acylation reaction-promoting agent is an acylation catalyst or acylation reaction-promoting agent selected from the group consisting of dialkylaminopyridines, cyclic tertiary organic amines, and phosphines.
[claim8]
8. The reagent according to claim 6, wherein the acylation catalyst or acylation reaction-promoting agent is dimethylaminopyridine, 1,4-diazabicyclo[2.2.2]octane, or tributylphosphine.
[claim9]
9. A method for visualizing an intracellular acetylation, which comprises the step of introducing a combination of a rhodamine derivative that is substantially non-fluorescent before being acetylated, and emits strong fluorescence after being acetylated, and an acylation catalyst and/or an acylation reaction-promoting agent into a cell, and the step of measuring fluorescence of the rhodamine derivative having been acetylated.
[claim10]
10. A rhodamine derivative that is substantially non-fluorescent before being acetylated, and emits strong fluorescence after being acetylated by an acylation catalyst and/or an acylation reaction-promoting agent.
[claim11]
11. A rhodamine derivative represented by the following formula (II), wherein R7, R8, R9, and R10 independently represent a C1-6 alkyl group and X- represents a counter ion.
00005
[claim12]
12. The rhodamine derivative according to claim 11, wherein each of R7, R8, R9, and R10 is methyl group.
  • 発明者/出願人(英語)
  • KANAI MOTOMU
  • KOMATSU HIROKAZU
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) ERATO KANAI Life Science Catalysis AREA
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