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Vector utilizing borna disease virus and use thereof

外国特許コード F170009065
掲載日 2017年5月26日
出願国 アメリカ合衆国
出願番号 201013258698
公報番号 20120151614
公報番号 9365865
出願日 平成22年3月17日(2010.3.17)
公報発行日 平成24年6月14日(2012.6.14)
公報発行日 平成28年6月14日(2016.6.14)
国際出願番号 JP2010054600
国際公開番号 WO2010113647
国際出願日 平成22年3月17日(2010.3.17)
国際公開日 平成22年10月7日(2010.10.7)
優先権データ
  • 特願2009-087608 (2009.3.31) JP
  • 2010WO-JP54600 (2010.3.17) WO
発明の名称 (英語) Vector utilizing borna disease virus and use thereof
発明の概要(英語) (US9365865)
Disclosed is a viral vector comprising (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome, and having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c).
特許請求の範囲(英語) [claim1]
1. A viral vector comprising: (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome,
having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene,
containing restriction enzyme sites both at the 3' end and at the 5' end of the foreign gene,
containing the sequence shown in SEQ ID NO: 11 (1) both in the region between the restriction enzyme site at the 3' end of the foreign gene and the open reading frame of the P gene and in the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the L gene, or (2) both in the region between the restriction enzyme site at the 3' end of the foreign gene and the open reading frame of the P gene and the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the M gene when the M gene of the Borna disease viral genome is contained in the same order as that in the Borna disease viral genome, and
containing at least a base sequence cc inserted in the region between the restriction enzyme site at the 3' end of the foreign gene and the sequence shown in SEQ ID NO: 11, and at least a base sequence cca in the region between the restriction enzyme site at the 5' end of the foreign gene and the sequence shown in SEQ ID NO: 11,
(b) a DNA encoding a ribozyme, and
(c) a promoter sequence,
each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c),
wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene and the M gene of the Borna disease virus genome are destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, and
wherein an intron of the L gene of the Borna disease virus genome is deleted from (a) the cDNA of a recombinant viral RNA.
[claim2]
2. The viral vector according to claim 1, wherein the restriction enzyme site at the 3' end of the foreign gene is a Bst BI site, and the restriction enzyme site at the 5' end of the foreign gene is a Pac I site.
[claim3]
3. The viral vector according to claim 1, wherein (c) the promoter sequence is an RNA polymerase II promoter sequence.
[claim4]
4. A kit comprising the viral vector according to claim 1.
[claim5]
5. The viral vector according to claim 1, wherein the foreign gene expresses a functional RNA.
  • 発明者/出願人(英語)
  • TOMONAGA KEIZO
  • DAITO TAKUJI
  • HONDA TOMOYUKI
  • KYOTO UNIVERSITY
国際特許分類(IPC)
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