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Vector utilizing borna disease virus and use thereof

外国特許コード F170009066
掲載日 2017年5月26日
出願国 欧州特許庁(EPO)
出願番号 10758423
公報番号 2415866
公報番号 2415866
出願日 平成22年3月17日(2010.3.17)
公報発行日 平成24年2月8日(2012.2.8)
公報発行日 平成28年8月10日(2016.8.10)
国際出願番号 JP2010054600
国際公開番号 WO2010113647
国際出願日 平成22年3月17日(2010.3.17)
国際公開日 平成22年10月7日(2010.10.7)
優先権データ
  • 特願2009-087608 (2009.3.31) JP
  • 2010WO-JP54600 (2010.3.17) WO
発明の名称 (英語) Vector utilizing borna disease virus and use thereof
発明の概要(英語) (EP2415866)
Disclosed is a viral vector comprising (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome, and having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c).
特許請求の範囲(英語) [claim1]
1. A viral vector comprising (a) a cDNA of a recombinant viral RNA
having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome,
having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene,
containing restriction enzyme sites both at the 3' end and at the 5' end of the foreign gene,
containing the sequences shown in SEQ ID NO: 11 both in the region between the restriction enzyme site at the 3' end of the foreign gene and the open reading frame of the P gene; and in the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the L gene, or the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the M gene when the M gene of the Borna disease viral genome is contained in the same order as that in the Borna disease viral genome, and
containing at least a base sequence cc inserted in the region between the restriction enzyme site at the 3' end of the foreign gene and the sequence shown in SEQ ID NO: 11, and at least a base sequence cca in the region between the restriction enzyme site at the 5' end of the foreign gene and the sequence shown in SEQ ID NO: 11 (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c).
[claim2]
2. The viral vector according to claim 1, wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene of the Borna disease virus genome is destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene.
[claim3]
3. The viral vector according to claim 1 or 2, wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene and the M gene of the Borna disease virus genome are destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene.
[claim4]
4. The viral vector according to any of claims 1 to 3, wherein the restriction enzyme site at the 3' end of the foreign gene is a Bst BI site, and the restriction enzyme site at the 5' end of the foreign gene is a Pac I site.
[claim5]
5. The viral vector according to any of claims 1 to 4, wherein (c) the promoter sequence is an RNA polymerase II promoter sequence.
[claim6]
6. The viral vector according to claim 2 or 3, wherein an intron of the L gene of the Borna disease virus genome is deleted from (a) the cDNA of a recombinant viral RNA.
[claim7]
7. A recombinant virus comprising an RNA encoded by the viral vector according to any of claims 1 to 6.
[claim8]
8. A process for preparing a recombinant virus, the process comprising the steps of
introducing, into an in vitro cell, a plasmid or a plasmid group expressing the N gene, the P gene, and the L gene of a Borna disease virus as a helper plasmid, together with the viral vector according to any of claims 1 to 6, and
culturing the cell having the introduced viral vector and helper plasmid to produce a recombinant virus.
[claim9]
9. The process for preparing a recombinant virus according to claim 8, wherein a plasmid expressing the coat gene of a virus is further introduced as a helper plasmid into the in vitro cell.
[claim10]
10. The process for preparing a recombinant virus according to claim 8 or 9, wherein a plasmid expressing the M gene of a Borna disease virus is further introduced as a helper plasmid into the in vitro cell.
[claim11]
11. A process for introducing a foreign gene, the process comprising the step of infecting an in vitro cell with the recombinant virus according to claim 7.
[claim12]
12. A foreign gene transfer agent comprising the recombinant virus according to claim 7.
[claim13]
13. A foreign gene transfer agent for a cranial nerve system cell, the agent comprising the recombinant virus according to claim 7.
[claim14]
14. A kit for introducing a foreign gene, the kit comprising the viral vector according to any of claims 1 to 6.
[claim15]
15. The viral vector according to any of claims 1 to 6, wherein the foreign gene is a DNA encoding a functional RNA.
  • 出願人(英語)
  • KYOTO UNIVERSITY
  • 発明者(英語)
  • TOMONAGA KEIZO
  • DAITO TAKUJI
  • HONDA TOMOYUKI
国際特許分類(IPC)
指定国 (EP2415866)
Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
上記の特許・技術に関心のある方は、下記「問合せ先」まで直接お問い合わせください。

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