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GENE EXPRESSION CASSETTE AND PRODUCT THEREOF

外国特許コード F170009080
整理番号 (S2016-0007-N0)
掲載日 2017年5月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP079219
国際公開番号 WO 2017061354
国際出願日 平成28年10月3日(2016.10.3)
国際公開日 平成29年4月13日(2017.4.13)
優先権データ
  • 特願2015-198160 (2015.10.6) JP
  • 特願2016-059297 (2016.3.23) JP
発明の名称 (英語) GENE EXPRESSION CASSETTE AND PRODUCT THEREOF
発明の概要(英語) The purpose of the present invention is to provide a gene expression cassette, for stable and high capacity production of a protein of interest. Provided is a gene expression cassette having a structure in which the gene of interest and a DNA construct (X) including a poly A addition sequence lies between a promoter (P) and an enhancer (P'), wherein the gene expression cassette is characterized by further comprising a transposon sequence (T), upstream of the promoter (P) and downstream of the enhancer (P'). Furthermore, in the foregoing, the protein of interest can more effectively be produced stably and in large quantities by combining and suitably arranging, in addition to the transposon sequence (T), a nuclear matrix binding sequence (M), upstream of a replication initiation sequence (S). For example, HRG, PD-1, EMMPRIN, NPTNβ, EMB, RAGE, MCAM, ALCAM, ErbB2, and antibodies can be produced stably and in large amounts.
特許請求の範囲(英語) [claim1]
1. Furthermore the promoter (P) high level and [enhansa] (P') transposon arrangement (T) it features that it includes in downstream the intended gene and DNA construction ones which include poly- A addition arrangement (X), the promoter (P) with [enhansa] (P') with in the cassette for gene revelation which possesses the structure which is put, the cassette for gene revelation.
[claim2]
2. Furthermore, the promoter (P) the high level and/or [enhansa] (P') in downstream, duplication start arrangement (S) it features that it is arranged, in claim 1 the cassette for gene revelation of statement.
[claim3]
3. The promoter (P), the intended gene and DNA construction ones which include poly- A addition arrangement (X), [enhansa] (P') and transposon arrangement (T) is included, furthermore is included in the order which selectively duplication start arrangement (S) the cassette for gene revelation which is included, 1) the -4 below) shows to in each case, in claim 1 or 2 the cassette for gene revelation of statement:
1) (T) (P), (X), (P') and (T);
2) (T) (S) and (P), (X), (P') and (T);
3) (T) (P), (X), (P'), (S) and (T);
4) (T) (S) and (P), (X), (P'), (S) and (T).
[claim4]
4. Duplication start arrangement (S) high-level nuclear matrix connection arrangement (M) in the claim 2 which features that it is arranged or 3 the cassette for gene revelation of statement.
[claim5]
5. Transposon arrangement (T), the promoter (P), the intended gene and DNA construction ones which include poly- A addition arrangement (X), [enhansa] (P'), nuclear matrix connection arrangement (M), duplication start arrangement (S) and transposon arrangement (T) it includes, the cassette for gene revelation.
[claim6]
6. Duplication start arrangement (S), ROIS and/or in either of the claim 2-5 which is ARS the cassette for gene revelation of statement.
[claim7]
7. The promoter (P), is the promoter who is selected from the group which consists of CMV promoter, the CMV-i promoter, the SV40 promoter, the hTERT promoter, the I actin promoter and the CAG promoter, in either of the claim 1-6 the cassette for gene revelation of statement.
[claim8]
8. The intended gene and DNA construction ones which include poly- A addition arrangement (X) [enhansa] which is connected to downstream (P'), in each case is selected from hTERT [enhansa], CMV [enhansa] and SV40 [enhansa] includes 1 kinds or plural kinds, in either of the claim 1-7 the cassette for gene revelation of statement.
[claim9]
9. The intended gene, the empty includes the gene which the part or the whole of the protein which is selected the cord/code is done HRG, PD-1, EMMPRIN, NPTN.beta., EMB, RAGE, MCAM, ALCAM, ErbB2 and either of the antibodies the intended gene and DNA construction ones which include poly- A addition arrangement (X) in, in either of the claim 1-8 the cassette for gene revelation of statement.
[claim10]
10. The cassette for gene revelation of statement is included in either of the claim 1-9, the plasmid for gene revelation.
[claim11]
11. The cassette for gene revelation of statement is included in either of the claim 1-9, the vector for gene revelation.
[claim12]
12. The method of making the intended gene either of the claim 1-9 reveal making use of the cassette for revelation of statement.
[claim13]
13. The protein which is produced to either of the claim 1-9 making use of the cassette for gene revelation of statement.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • OKAYAMA UNIVERSITY
  • 発明者(英語)
  • SAKAGUCHI MASAKIYO
  • NISHIBORI MASAHIRO
  • KUMON HIROMI
  • MURATA HITOSHI
  • YAMAMOTO KENICHI
  • KINOSHITA RIE
国際特許分類(IPC)
指定国 (WO201761354)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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