TOP > クイック検索 > 外国特許検索 > RECOMBINANT EXPRESSION VECTOR AND LIPID MEMBRANE STRUCTURE HAVING SAID VECTOR ENCAPSULATED THEREIN

RECOMBINANT EXPRESSION VECTOR AND LIPID MEMBRANE STRUCTURE HAVING SAID VECTOR ENCAPSULATED THEREIN

外国特許コード F170009107
整理番号 (S2016-0168-N0)
掲載日 2017年6月15日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP085098
国際公開番号 WO 2017090763
国際出願日 平成28年11月28日(2016.11.28)
国際公開日 平成29年6月1日(2017.6.1)
優先権データ
  • 特願2015-230498 (2015.11.26) JP
発明の名称 (英語) RECOMBINANT EXPRESSION VECTOR AND LIPID MEMBRANE STRUCTURE HAVING SAID VECTOR ENCAPSULATED THEREIN
発明の概要(英語) [Problem] To provide a novel expression vector capable of effectively expressing a target protein in mitochondria and suppressing undesirable expression of the target protein in cell organelles other than mitochondria. [Solution] The present invention provides a recombinant expression vector for expressing a target protein in mitochondria of animal cells, and a lipid membrane structure having the vector encapsulated therein, wherein the recombinant expression vector has a promoter sequence exhibiting a transcription activity in the nuclei of animal cells, and has, under the control of the promoter sequence, a coding region which codes a target protein and includes one or more TGAs as codons corresponding to tryptophan. The recombinant expression vector according to the present invention can more efficiently and selectively express a target protein in mitochondria, and can be used as a more safe and effective drug for treating mitochondrial diseases.
特許請求の範囲(英語) [claim1]
1. The cell of the animal cell being nuclear, being the coding territory which the cord/code it does promoter arrangement, and the intended protein which show copying activity, the rearrangement manifestation vector in order one or possesses the coding territory which includes plural TGA under controlling the aforementioned promoter arrangement as the codon which corresponds to toriputohuan, to make the intended protein reveal inside the mitochondria of the animal cell.
[claim2]
2. 5' on terminal side of the coding territory which the aforementioned intended protein the cord/code is done, furthermore it possesses the coding territory of mitochondria genome DNA, in claim 1 the rearrangement manifestation vector of statement.
[claim3]
3. It possesses the base arrangement which corresponds to mitochondria tRNA 3' on terminal side of the coding territory which the aforementioned intended protein the cord/code is done, in claim 1 or 2 the rearrangement manifestation vector of statement.
[claim4]
4. It is base arrangement of the promoter who is selected from the group where promoter arrangement consists of saitomegarouirusupuromota, the simian virus 40 promoter, the louse monkey comber virus promoter, the EF1.alpha. promoter, the I actin promoter and the T7 promoter, in either of the claim 1-3 the rearrangement manifestation vector of statement.
[claim5]
5. Promoter arrangement is base arrangement of the saitomegarouirusupuromota or louse monkey comber virus promoter, in claim 4 the rearrangement manifestation vector of statement.
[claim6]
6. Everything of the codon which corresponds to toriputohuan in the coding territory which the aforementioned intended protein the cord/code is done is TGA, in either of the claim 1-5 the rearrangement manifestation vector of statement.
[claim7]
7. The coding territory of the aforementioned mitochondria genome DNA NADH dehydrogenase, is the coding territory of sub unit 4, in either of the claim 2-6 the rearrangement manifestation vector of statement.
[claim8]
8. The lipid membrane structure which encloses the manifestation vector of statement into either of the claim 1-7.
[claim9]
9. It includes suhuingomierin as the constitution lipid of the lipid membrane in claim 8 the lipid membrane structure of statement.
[claim10]
10. It possesses the peptide which consists of the amino acid arrangement which is shown with arrangement number 13 on the lipid membrane surface, in claim 8 or 9 the lipid membrane structure of statement.
[claim11]
11. Possesses the peptide which consists of the amino acid arrangement which at the same time is shown with arrangement number 13 on the lipid membrane surface, to introduce nuclear acid into the mitochondria of the animal cell the lipid membrane structure in order it contains GeoCities lane phosphatidyl ethanolamine and suhuingomierin as the constitution lipid of the lipid membrane.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • HOKKAIDO UNIVERSITY
  • 発明者(英語)
  • HARASHIMA HIDEYOSHI
  • YAMADA YUMA
  • ISHIKAWA TAKUYA
  • AKITA HIDETAKA
国際特許分類(IPC)
指定国 (WO201790763)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
ライセンスをご希望の方、特許の内容に興味を持たれた方は、下記までご連絡ください

PAGE TOP

close
close
close
close
close
close