TOP > 外国特許検索 > DIFFERENTIATION INDUCTION FROM HUMAN PLURIPOTENT STEM CELLS INTO HYPOTHALAMIC NEURONS

DIFFERENTIATION INDUCTION FROM HUMAN PLURIPOTENT STEM CELLS INTO HYPOTHALAMIC NEURONS NEW

外国特許コード F170009139
整理番号 (S2016-0324-N0)
掲載日 2017年8月8日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP001544
国際公開番号 WO 2017126551
国際出願日 平成29年1月18日(2017.1.18)
国際公開日 平成29年7月27日(2017.7.27)
優先権データ
  • 特願2016-010940 (2016.1.22) JP
発明の名称 (英語) DIFFERENTIATION INDUCTION FROM HUMAN PLURIPOTENT STEM CELLS INTO HYPOTHALAMIC NEURONS NEW
発明の概要(英語) Provided is a method for efficiently inducing differentiation from human pluripotent stem cells into hypothalamic neurons. Also provided is a method for constructing a cellular structure that integrates pituitary tissue and hypothalamic tissue from human pluripotent stem cells. A cellular structure including hypothalamic tissue is obtained by a method including a step for suspension culturing human pluripotent stem cell spheroids in a medium including a low concentration of an osteogenic factor signaling pathway activator and a low concentration of a substance capable of acting on the Shh signaling pathway, and a step for also suspension culturing the cell spheroids obtained in that step in a medium including a low concentration of a substance capable of acting on the Shh signaling pathway. A cellular structure including hypothalamic tissue and pituitary tissue is also obtained by a method including a step for suspension culturing human pluripotent stem cell spheroids in a medium including an osteogenic factor signaling pathway activator and a substance capable of acting on the Shh signaling pathway, a step for also suspension culturing the cell spheroids formed in that step in a medium including an osteogenic factor signaling pathway activator and a substance capable of acting on the Shh signaling pathway, and a step for suspension culturing the cell spheroids obtained in that step in a medium suitable for simultaneous pituitary and hypothalamic induction .
特許請求の範囲(英語) [claim1]
1. Production method of the cell structure which step below includes (1) and (2), includes thalamus subsystem:
(1) the cohesion lump of the human versatile characteristic trunk cell, it is low the bone formation factor signal transmission route activated substance of density and low the step which it floats cultures in the nutrient medium which includes the Shh signal route active substance of density,
(2) is obtained with step (1) the cell cohesion lump which, low the step which furthermore it floats cultures in the nutrient medium which includes the Shh signal route active substance of density.
[claim2]
2. Step (2) is done under high oxygen partial pressure condition, in claim 1 production method of statement.
[claim3]
3. Step below (3) furthermore is included, in claim 1 or 2 production method of statement:
(3) is obtained with, step (2) the step which it disperses cultures the cell which collects the cell cohesion lump which forms the cell cohesion lump.
[claim4]
4. Step (3) is done under high oxygen partial pressure condition, in claim 3 production method of statement.
[claim5]
5. In step (1) the aforementioned bone formation factor signal transmission route activated substance is BMP4, the density in the nutrient medium is 0.1 nM-5.0 nM,
In step (1) and (2) the aforementioned Shh signal route active substance is SAG, the density in the nutrient medium is 0.1 .micro. M-2.0 .micro. M,
Differentiation to dorsal thalamus subsystem is induced by the aforementioned step (1) and (2),
Either of claim 1-4 in one section production method of statement.
[claim6]
6. The aforementioned dorsal thalamus subsystem includes the neuron of one or more which is selected from the group which consists of bazopureshinniyuron, the oxytocin neuron, the thyroid stimulus hormone discharge hormone neuron, the corticotropin discharge hormone neuron and the neuro peptide Y neuron, in claim 5 production method of statement.
[claim7]
7. In step (1) the aforementioned bone formation factor signal transmission route activated substance is BMP4, the density in the nutrient medium is 0.1 nM-3.0 nM,
In step (1) and (2) the aforementioned Shh signal route active substance is SAG, the density in the nutrient medium is 0.1 .micro. M-2.0 .micro. M,
The aforementioned nutrient medium Akt inhibiter furthermore implication,
Differentiation to ventral thalamus subsystem is induced by the aforementioned step (1) and (2), either of the claim 1-4 in one section production method of statement.
[claim8]
8. The aforementioned ventral thalamus subsystem the aguchi-related protein neuron, includes the neuron of one or more which is selected from the group which consists of puroopiomeranokoruchinniyuron, the melanin cohesion hormone neuron and the Orexin neuron, in claim 7 production method of statement.
[claim9]
9. The aforementioned floating culture is done under non existing of the feeder cell, either of the claim 1-8 in one section production method of statement.
[claim10]
10. In step (1) the aforementioned cohesion lump, is formed by the floating culture of the human versatile characteristic trunk cell which is made to disperse, either of the claim 1-9 in one section production method of statement.
[claim11]
11. The aforementioned floating culture bloodless is done clearly with cohesion floating method of cultivation, in claim 10 production method of statement.
[claim12]
12. Production method of the cell structure which includes the step (i)-(iii) below, includes thalamus subsystem and pituitary gland organization:
(i) The cohesion lump of the human versatile characteristic trunk cell, the step which it floats cultures in the nutrient medium which includes the bone formation factor signal transmission route activated substance and the Shh signal route active substance,
(ii) The cell cohesion lump which was formed with step (i), the step which furthermore it floats cultures in the nutrient medium which includes the bone formation factor signal transmission route activated substance and the Shh signal route active substance,
(iii) The cell cohesion lump which is obtained with step (ii), the step which it floats cultures the pituitary gland and in the nutrient medium which is suited for the simultaneous induction of thalamus lower part
[claim13]
13. It does step (ii) and (iii) under high oxygen partial pressure condition, in claim 12 production method of statement.
[claim14]
14. Step below (a) it does between step (ii) and step (iii), with step (iii) step (a) with it floats it cultures the cell cohesion lump which is obtained, in claim 12 or 13 production method of statement:
(A) The cell cohesion lump which is obtained with step (ii), the step which furthermore it floats cultures in the nutrient medium which includes the Shh signal route active substance.
[claim15]
15. Step (a) it does under high oxygen partial pressure condition, either of the claim 12-14 in one section production method of statement.
[claim16]
16. The aforementioned floating culture is done under non existing of the feeder cell, either of the claim 12-15 in one section production method of statement.
[claim17]
17. The aforementioned cohesion lump in step (i), is formed by the floating culture of the human versatile characteristic trunk cell which is made to disperse, either of the claim 12-16 in one section production method of statement.
[claim18]
18. The bone formation factor signal transmission route activated substance is BMP4, either of the claim 12-17 in one section production method of statement.
[claim19]
19. The Shh signal route active substance is SAG, either of the claim 12-18 in one section production method of statement.
[claim20]
20. Either of the claim 1-19 the cell structure which in one section is obtained with production method of statement.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NAGOYA UNIVERSITY
  • 発明者(英語)
  • SUGA HIDETAKA
  • OGAWA KOICHIRO
  • KASAI TAKATOSHI
  • ARIMA HIROSHI
国際特許分類(IPC)
指定国 (WO2017126551)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
名古屋大学の公開特許情報を掲載しています。ご関心のある案件がございましたら、下記まで電子メールでご連絡ください。

PAGE TOP

close
close
close
close
close
close