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METHOD FOR PROMOTING FERMENTATION THROUGH LOSS OF FUNCTION OF VACUOLAR TRANSPORTER CHAPERONE COMPLEX OF YEAST UPDATE

外国特許コード F170009153
整理番号 (S2016-0382-N0)
掲載日 2017年8月24日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP004212
国際公開番号 WO 2017138489
国際出願日 平成29年2月6日(2017.2.6)
国際公開日 平成29年8月17日(2017.8.17)
優先権データ
  • 特願2016-025324 (2016.2.12) JP
発明の名称 (英語) METHOD FOR PROMOTING FERMENTATION THROUGH LOSS OF FUNCTION OF VACUOLAR TRANSPORTER CHAPERONE COMPLEX OF YEAST UPDATE
発明の概要(英語) The present invention pertains to a method for enhancing the fermentation capacity and ethanol yield of yeast. Specifically, the present invention pertains to: a method for enhancing the fermentation capacity of yeast that involves causing the decrease or loss of the vacuolar transporter chaperone complex (VTC complex) function in yeast; a fermentation product production method using yeast that involves carrying out alcoholic fermentation in conditions wherein the VTC complex function in the yeast is decreased or lost; a fermentation yeast preparation method that involves causing the decrease or loss of the VTC complex function in yeast by introducing a genetic mutation; yeast wherein the VTC complex function has been decreased or lost due to genetic mutation; and a screening method for drugs that enhance the fermentation capacity of yeast.
特許請求の範囲(英語) [claim1]
1. In the yeast, the liquid placenta trance porter chaperon mixture (the VTC mixture) the fact that it decreases or loses function, or is included, the method fermentation talent of the yeast improving.
[claim2]
2. Decrease or loss of function of the VTC mixture of the yeast, is something due to the formation obstruction of the VTC mixture which is formed from plural constitution factors, the method of improving the fermentation talent of the yeast of statement to claim 1.
[claim3]
3. Decrease or loss of function of the VTC mixture of the yeast, by introducing gene mutation in the yeast, is something due to at least decreasing the revelation quantity of one which is selected from the gene which constitution factor of the VTC mixture the cord/code is done gene, the method of improving the fermentation talent of the yeast of statement in claim 1 or 2.
[claim4]
4. The gene mutation in the yeast, at least is destruction of one which is selected from the gene which constitution factor of the VTC mixture the cord/code is done gene, the method of improving the fermentation talent of the yeast of statement to claim 3.
[claim5]
5. At least it is one where at least one which from the gene which constitution factor of the VTC mixture the cord/code is done is selected gene, is selected from the group which consists of the VTC1 gene, the VTC2 gene, the VTC3 gene and the VTC4 gene gene, the method of improving the fermentation talent of the yeast of statement in claim 3 or 4.
[claim6]
6. At least it is one which is selected from the group where one which from the gene which constitution factor of the VTC mixture the cord/code is done is selected gene consists of, (a) below the - (e) at least gene, the method of improving the fermentation talent of the yeast of statement in either of the claim 2-5:
(A) In either of the arrangement number 1-4 the gene which includes the polynucleotide which amino acid arrangement of statement the cord/code is done;
(B) The gene which includes the polynucleotide which the amino acid arrangement where 1 or the amino acid residue several or more is deficient in either of the arrangement number 1-4 at the time of amino acid arranging of statement, substitution and insertion and/or is added the cord/code is done;
(C) In either of the arrangement number 1-4 the gene which includes the polynucleotide which amino acid arrangement of statement and the amino acid arrangement which possesses homology of 80% or more the cord/code is done;
(D) The gene which includes the polynucleotide which consists of the base arrangement of statement in either of the arrangement number 5-8;
(E) Description above (a) the - (d) no polynucleotide which is selected from the group which consists of the gene which includes the polynucleotide which consists of complimentary base arrangement.
[claim7]
7. The method of improving the fermentation talent of the yeast of statement in either of the claim 1-6 is included, being the method of producing the fermentation product making use of the yeast, in the yeast under the conditions function of the VTC mixture having decreased or losing, it includes the fact that it ferments alcoholically, production method of the fermentation product.
[claim8]
8. The fermentation product is the ethanol, in claim 7 production method of the fermentation product of statement.
[claim9]
9. The fermentation product is the fermentation beverage or the fermentation food, in claim 7 production method of the fermentation product of statement.
[claim10]
10. The fact that it decreases or loses function of the VTC mixture, or is included in the yeast, by introducing gene mutation, production method of the yeast for vinification.
[claim11]
11. With gene mutation, function of constitution factor of the VTC mixture or the VTC mixture decreased or lost, or, the yeast for vinification.
[claim12]
12. Gene mutation, at least is destruction of one which is selected from the gene which constitution factor of the VTC mixture the cord/code is done gene, in claim 11 the yeast for vinification of statement.
[claim13]
13. With gene mutation, the yeast which is the yeast which function of the VTC mixture decreases or loses, or, is either the brewing yeast, the baker's yeast or the bio ethanol yeast.
[claim14]
14. The yeast of statement is contained in either of the claim 11-13, the composition for vinification.
[claim15]
15. Being screening method of the medicine in order to improve the fermentation talent of the yeast, it includes the process which operates suffering chemical compound the VTC mixture, particular suffering chemical compound designates that it decreases or loses function of the VTC mixture, or as index, screening method.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
  • 発明者(英語)
  • WATANABE DAISUKE
  • TAKAGI KENICHI
  • TAKAGI HIROSHI
国際特許分類(IPC)
指定国 (WO2017138489)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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