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Nucleic acid-encapsulating polymer micelle complex and method for producing same UPDATE

外国特許コード F170009169
整理番号 K10503WO
掲載日 2017年9月12日
出願国 カナダ
出願番号 2920328
公報番号 2920328
公報番号 2920328
出願日 平成26年8月5日(2014.8.5)
公報発行日 平成27年2月12日(2015.2.12)
公報発行日 平成29年10月10日(2017.10.10)
国際出願番号 JP2014070567
国際公開番号 WO2015020026
国際公開日 平成27年2月12日(2015.2.12)
優先権データ
  • 特願2013-163106 (2013.8.6) JP
  • 2014WO-JP70567 (2014.8.5) WO
発明の名称 (英語) Nucleic acid-encapsulating polymer micelle complex and method for producing same UPDATE
発明の概要(英語) (CA2920328)
This nucleic acid-encapsulating polymer micelle complex is characterized in being formed of: a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block; and two single-stranded DNA molecules comprising mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of the double helix structure has dissociated and taken on a single-stranded structure, or one single-stranded DNA molecule of 1000 or more bases in length.
特許請求の範囲(英語) [claim1]
1. A nucleic acid-encapsulating polymer micelle complex formed of a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block;
and two single-stranded DNAs having mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure, or one single-stranded DNA of 1000 or more bases in length.
[claim2]
2. The nucleic acid-encapsulating polymer micelle complex according to
Claim 1, formed of a block copolymer containing an uncharged hydrophilic chain block and a cationic polymer chain block;
and two single-stranded DNAs having mutually complementary base sequences of 1000 or more bases in length or doublestranded DNA
of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure.
[claim3]
3. The nucleic acid-encapsulating polymer micelle complex according to
Claim 1 or 2, wherein the single-stranded DNA is 2000 or more bases in length, and the double-stranded DNA is 2000 or more base pairs in length.
[claim4]
4. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 3, wherein the average particle diameter thereof in an aqueous medium measured according to a dynamic light scattering method is 100 nm or less.
[claim5]
5. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 4, wherein the DNA and the cationic polymer chain block bonded to the DNA due to an electrostatic interaction form a core portion, and the uncharged hydrophilic polymer chain block forms a shell portion.
[claim6]
6. The nucleic acid-encapsulating polymer micelle complex according to
Claim 5, wherein the average particle diameter of the core portion is 50 nm or less.
[claim7]
7. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 6, wherein the complex is spherical.
[claim8]
8. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 7, wherein the single-stranded DNA or the double-stranded
DNA is linear.
[claim9]
9. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 8, wherein at least a part of the block copolymer is mutually cross-linked.
[claim10]
10. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 9, wherein a hydrophobic group is covalently bonded to a main chain or a side chain of the cationic polymer chain block.
[claim11]
11. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 10, wherein the cationic polymer chain block has an ethylamine structure or a propylamine structure in the side chain thereof
[claim12]
12. A method for producing a nucleic acid-encapsulating polymer micelle complex which accommodates DNA, the method comprising:
a process of mixing a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block with double-stranded
DNA of
1000 or more base pairs in a state in which at least a part of a double helix structure is dissociated, in an aqueous medium.
[claim13]
13. The method for producing a nucleic acid-encapsulating polymer micelle complex according to Claim 12, wherein the double-stranded DNA is 2000 or more base pairs in length.
[claim14]
14. The method for producing a nucleic acid-encapsulating polymer micelle complex according to Claim 12 or 13, wherein the double-stranded DNA is linear.
[claim15]
15. The method for producing a nucleic acid-encapsulating polymer micelle complex according to any one of Claims 12 to 14, wherein the double-stranded
DNA has been denatured at 60.deg.C or higher.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • KATAOKA KAZUNORI
  • OSADA KENSUKE
  • TOCKARY THEOFILUS AGRIOS
国際特許分類(IPC)
参考情報 (研究プロジェクト等) PRESTO Molecular technology and creation of new functions AREA
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