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Nucleic acid-encapsulating polymer micelle complex and method for producing same

Foreign code F170009169
File No. K10503WO
Posted date Sep 12, 2017
Country Canada
Application number 2920328
Gazette No. 2920328
Date of filing Aug 5, 2014
Gazette Date Feb 12, 2015
International application number JP2014070567
International publication number WO2015020026
Date of international filing Aug 5, 2014
Date of international publication Feb 12, 2015
Priority data
  • P2013-163106 (Aug 6, 2013) JP
  • 2014WO-JP70567 (Aug 5, 2014) WO
Title Nucleic acid-encapsulating polymer micelle complex and method for producing same
Abstract (CA2920328)
This nucleic acid-encapsulating polymer micelle complex is characterized in being formed of: a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block; and two single-stranded DNA molecules comprising mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of the double helix structure has dissociated and taken on a single-stranded structure, or one single-stranded DNA molecule of 1000 or more bases in length.
Scope of claims [claim1]
1. A nucleic acid-encapsulating polymer micelle complex formed of a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block;
and two single-stranded DNAs having mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure, or one single-stranded DNA of 1000 or more bases in length.
[claim2]
2. The nucleic acid-encapsulating polymer micelle complex according to
Claim 1, formed of a block copolymer containing an uncharged hydrophilic chain block and a cationic polymer chain block;
and two single-stranded DNAs having mutually complementary base sequences of 1000 or more bases in length or doublestranded DNA
of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure.
[claim3]
3. The nucleic acid-encapsulating polymer micelle complex according to
Claim 1 or 2, wherein the single-stranded DNA is 2000 or more bases in length, and the double-stranded DNA is 2000 or more base pairs in length.
[claim4]
4. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 3, wherein the average particle diameter thereof in an aqueous medium measured according to a dynamic light scattering method is 100 nm or less.
[claim5]
5. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 4, wherein the DNA and the cationic polymer chain block bonded to the DNA due to an electrostatic interaction form a core portion, and the uncharged hydrophilic polymer chain block forms a shell portion.
[claim6]
6. The nucleic acid-encapsulating polymer micelle complex according to
Claim 5, wherein the average particle diameter of the core portion is 50 nm or less.
[claim7]
7. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 6, wherein the complex is spherical.
[claim8]
8. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 7, wherein the single-stranded DNA or the double-stranded
DNA is linear.
[claim9]
9. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 8, wherein at least a part of the block copolymer is mutually cross-linked.
[claim10]
10. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 9, wherein a hydrophobic group is covalently bonded to a main chain or a side chain of the cationic polymer chain block.
[claim11]
11. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 10, wherein the cationic polymer chain block has an ethylamine structure or a propylamine structure in the side chain thereof
[claim12]
12. A method for producing a nucleic acid-encapsulating polymer micelle complex which accommodates DNA, the method comprising:
a process of mixing a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block with double-stranded
DNA of
1000 or more base pairs in a state in which at least a part of a double helix structure is dissociated, in an aqueous medium.
[claim13]
13. The method for producing a nucleic acid-encapsulating polymer micelle complex according to Claim 12, wherein the double-stranded DNA is 2000 or more base pairs in length.
[claim14]
14. The method for producing a nucleic acid-encapsulating polymer micelle complex according to Claim 12 or 13, wherein the double-stranded DNA is linear.
[claim15]
15. The method for producing a nucleic acid-encapsulating polymer micelle complex according to any one of Claims 12 to 14, wherein the double-stranded
DNA has been denatured at 60.deg.C or higher.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • KATAOKA KAZUNORI
  • OSADA KENSUKE
  • TOCKARY THEOFILUS AGRIOS
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO Molecular technology and creation of new functions AREA
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