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Nucleic acid-encapsulating polymer micelle complex and method for producing same

Foreign code F170009170
File No. K10503WO
Posted date Sep 12, 2017
Country Republic of Korea
Application number 20167001466
Gazette No. 20160021291
Gazette No. 101770705
Date of filing Aug 5, 2014
Gazette Date Feb 24, 2016
Gazette Date Aug 23, 2017
International application number JP2014070567
International publication number WO2015020026
Date of international filing Aug 5, 2014
Date of international publication Feb 12, 2015
Priority data
  • P2013-163106 (Aug 6, 2013) JP
  • 2014WO-JP70567 (Aug 5, 2014) WO
Title Nucleic acid-encapsulating polymer micelle complex and method for producing same
Abstract (KR101770705)
A nucleic acid-encapsulating polymer micelle complex is formed of a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block; and two single-stranded DNA molecules having mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure, or one single-stranded DNA molecule of 1000 or more bases in length.
(From EP3031447 A1)
Scope of claims [claim1]
1. As for under including block copolymers and 1000 salty tastes 2 forever above each other become accomplished with the base sequence which is tablecloth 1 sway DNA and double the part of the helix structure is dissociated the charge-mass ratio hydrophilic high-molecular sway block and the positive ion high-molecular sway block, 1000 base pair which become 1 sway structure forever 1 with 1 sway DNA of above, forever 2 sway DNA or 1000 salty tastes of above is formed is become accomplished and, is a specific form the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim2]
2. There is in a 1st claim,
As for under including block copolymers and 1000 salty tastes 2 forever above each other become accomplished with the base sequence which is tablecloth 1 sway DNA or double the part of the helix structure is dissociated the charge-mass ratio hydrophilic high-molecular sway block and the positive ion high-molecular sway block, 1000 base pair which are becoming with 1 sway structure forever with 2 sway DNA of above, is formed is become accomplished the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim3]
3. The 1st claim or there is in a 2nd claim,
As for minute description 1 sway DNA 2000 salty tastes to be an above forever, and as for minute description 2 sway DNA 2000 base pair forever is an above the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim4]
4. The 1st claim or there is in a 2nd claim,
The average particle diameter by the dynamic storehouse scattering method in hydrogenous medium is below 100 the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim5]
5. The 1st claim or there is in a 2nd claim,
By DNA and a power failure interaction with DNA the positive ion high-molecular sway block which combined forms a core part, and the charge-mass ratio hydrophilic high-molecular sway block is forming a shell part the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim6]
6. There is in a 5th claim,
Average particle diameter of minute description core part is below 50 the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim7]
8. The 1st claim or there is in a 2nd claim,
As for minute description 1 sway DNA or minute description 2 sway DNA is a line the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim8]
9. The 1st claim or there is in a 2nd claim,
As for part of minute description block copolymer each other is building a bridge the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim9]
10. The 1st claim or there is in a 2nd claim,
To week sway or the branched chain of the minute description positive ion high-molecular sway block hydrophoby giga is covalent bond the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim10]
11. The 1st claim or there is in a 2nd claim,
Has an ethyl amine structure or a profile amine structure to the branched chain of the minute description positive ion high-molecular sway block the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim11]
12. The nuclear acid containment high-molecular micelle complex which accommodated DNA as for under manufacturing as method,
The charge-mass ratio hydrophilic high-molecular sway block and the positive ion high-molecular sway block as for under including block copolymer and double part of the helix structure at the time of Harry 2 sway DNA of above 1000 base pair of Keane situation the low of mixing includes a process from in hydrogenous medium,
As for the minute description nuclear acid containment high-molecular micelle complex is a specific form manufacturing method of the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim12]
13. There is in a 12th claim,
As for minute description 2 sway DNA 2000 base pair forever is an above manufacturing method of the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim13]
14. The 12th claim or there is in a 13th claim,
As for minute description 2 sway DNA is a line manufacturing method of the nuclear acid containment high-molecular micelle complex the fact that with feature.
[claim14]
15. The 12th claim or there is in a 13th claim,
As for minute description 2 sway DNA metamorphose from more than 60.deg.C manufacturing method of the nuclear acid containment high-molecular micelle complex the fact that with feature.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • KATAOKA KAZUNORI
  • OSADA KENSUKE
  • TOCKARY THEOFILUS AGRIOS
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO Molecular technology and creation of new functions AREA
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