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Nucleic acid-encapsulating polymer micelle complex and method for producing same UPDATE_EN

Foreign code F170009172
File No. K10503WO
Posted date Sep 12, 2017
Country China
Application number 201480043775
Gazette No. 105451719
Date of filing Aug 5, 2014
Gazette Date Mar 30, 2016
International application number JP2014070567
International publication number WO2015020026
Date of international filing Aug 5, 2014
Date of international publication Feb 12, 2015
Priority data
  • P2013-163106 (Aug 6, 2013) JP
  • 2014WO-JP70567 (Aug 5, 2014) WO
Title Nucleic acid-encapsulating polymer micelle complex and method for producing same UPDATE_EN
Abstract (CN105451719)
This nucleic acid-encapsulating polymer micelle complex is characterized in being formed of: a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block; and two single-stranded DNA molecules comprising mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of the double helix structure has dissociated and taken on a single-stranded structure, or one single-stranded DNA molecule of 1000 or more bases in length.
Scope of claims [claim1]
1. A polymer microcapsule complex one-containing nucleic acid, characterized in, non-hydrophilic polymer that contains cationic polymer chain embedded segment and be shifted a block copolymer chain embedded, comprising not less than 1000 bases in length with a base sequence complementary to each other single-stranded DNA 2 are, at least a portion capable of being separated from each other 1000 base pairs in length or more double-stranded structure of a DNA, or 1000 bases in length or more DNA strand 1 is formed.
[claim2]
2. A polymer microcapsule encapsulated nucleic acid complex according to claim 1, that contains a hydrophilic polymer chain embedded segment and non-chargeability cationic polymer chain embedded segmented block copolymer, containing not less than 1000 bases in length complementary to each other with the base sequence of DNA single strands 2, or at least a portion of the duplex structure dissociates into a single structure of double-stranded DNA at least 1000 base pairs in length are formed.
[claim3]
3. Nucleic acid-encapsulating microcapsule polymer composite according to claim 1 or 2, wherein, single-stranded DNA to 2000 bases in length or more, double-stranded DNA to 2000 base pairs in length or more.
[claim4]
4. Claim 1-3 of a nucleic acid according to any one microcapsule encapsulating polymer composite, which is obtained by a dynamic light scattering method in an aqueous medium average particle diameter of 100 nm or less.
[claim5]
5. Claim 1-4 according to any one of a nucleic acid-containing polymer microcapsule complex, wherein, by an electrostatic interaction with DNA and a DNA-bound cationic polymer chain embedded segment forming the core portion, the hydrophilic polymer chain embedded segment forming the shell part non-chargeability.
[claim6]
6. Nucleic acid-encapsulating microcapsule polymer composite according to claim 5, wherein, an average particle diameter of 50 nm or less core portion.
[claim7]
7. In accordance with claim 1-6 microcapsule encapsulating polymer composite according to any one of a nucleic acid, which is spherical.
[claim8]
8. Claim 1-7 of a nucleic acid according to any one microcapsule encapsulating polymer composite, wherein, single-stranded DNA or double-stranded DNA is a linear shape.
[claim9]
9. In accordance with claim 1-8 a polymer microcapsules included in any one of nucleic acid complex, wherein, at least part of block copolymer cross-linked with each other.
[claim10]
10. Claim 1-9 encapsulated nucleic acid according to any one of a polymer microcapsule complex, wherein, in the cationic polymer main chain or side chain embedded in covalent bond with the hydrophobic segment of the base.
[claim11]
11. Claim 1-10 of a nucleic acid according to any one microcapsule encapsulating polymer composite, wherein, in a cationic polymer having an amine structure or a side chain of polyallylamine chain embedded segment structure.
[claim12]
12. One method of producing a composite polymer of a nucleic acid-encapsulating microcapsules, characterized in, which is made of a nucleic acid containing the DNA polymer microcapsules from the group of a complex method,
This method comprises the following step: contains the non-hydrophilic polymer chain embedded segment and a cationic polymer be shifted a block copolymer chain embedded, with the duplex structure of at least a portion of the dissociated state of a double-stranded DNA 1000 base pairs or more mixed in an aqueous medium.
[claim13]
13. Nucleic acid polymer microcapsules encapsulating method for manufacturing a composite according to claim 12, wherein, double-stranded DNA to 2000 base pairs in length or more.
[claim14]
14. Nucleic acid polymer microcapsules encapsulating method for manufacturing a composite according to claim 12 or 13, wherein, double-stranded DNA is a linear shape.
[claim15]
15. Claim 12-14 of a nucleic acid according to any one method of producing a composite microcapsules encapsulating polymer, wherein, in the double-stranded DNA was denatured by a double-stranded DNA was not less than 60 °C.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • KATAOKA KAZUNORI
  • OSADA KENSUKE
  • TOCKARY THEOFILUS AGRIOS
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO Molecular technology and creation of new functions AREA
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