TOP > 外国特許検索 > Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA

Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA NEW

外国特許コード F170009174
整理番号 I029P002EP1
掲載日 2017年9月12日
出願国 欧州特許庁(EPO)
出願番号 12002217
公報番号 2500427
公報番号 2500427
出願日 平成20年11月21日(2008.11.21)
公報発行日 平成24年9月19日(2012.9.19)
公報発行日 平成26年7月30日(2014.7.30)
優先権データ
  • 特願2007-303661 (2007.11.22) JP
  • 2008EP-0852782 (2008.11.21) EP
発明の名称 (英語) Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA NEW
発明の概要(英語) (EP2500427)
The present invention is related to a liposome comprising an mRNA or DNA and a cell-free translational system encapsulated therein.
特許請求の範囲(英語) [claim1]
1. A liposome comprising an mRNA or DNA and a cell-free translational system encapsulated therein.
[claim2]
2. A method for producing a liposome comprising an mRNA or DNA and a cell-free translational system encapsulated therein, comprising the steps of: mixing one or more phospholipids, the mRNA or DNA, the cell-free translational system, and an aqueous solution into an oily liquid to form a W/O emulsion in which the mRNA or DNA and the cell-free translational system are encapsulated in the phospholipid vesicle; adding an oily liquid containing outer membrane lipids dissolved therein, to an aqueous phase to form a molecular membrane in which the lipids are arranged at the oil/water interface; and adding the W/O emulsion to the oil phase side of the interface and moving the W/O emulsion to the aqueous phase side of the interface such that the outer membrane lipid is added outside of the W/O emulsion to form a liposome.
[claim3]
3. A method for real-time monitoring of intraliposomal protein translation reaction, comprising the step of microscopically observing the liposome after the liposome formation step according to claim 2.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • INOUE TAN
  • SAITO HIROHIDE
  • KATO YUSHO
  • YOSHIKAWA KENICHI
  • YAMADA AYAKO
  • YAMANAKA TORU
国際特許分類(IPC)
指定国 (EP2500427)
Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
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