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Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA

Foreign code F170009174
File No. I029P002EP1
Posted date Sep 12, 2017
Country EPO
Application number 12002217
Gazette No. 2500427
Gazette No. 2500427
Date of filing Nov 21, 2008
Gazette Date Sep 19, 2012
Gazette Date Jul 30, 2014
Priority data
  • P2007-303661 (Nov 22, 2007) JP
  • 2008EP-0852782 (Nov 21, 2008) EP
Title Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA
Abstract (EP2500427)
The present invention is related to a liposome comprising an mRNA or DNA and a cell-free translational system encapsulated therein.
Scope of claims [claim1]
1. A liposome comprising an mRNA or DNA and a cell-free translational system encapsulated therein.
[claim2]
2. A method for producing a liposome comprising an mRNA or DNA and a cell-free translational system encapsulated therein, comprising the steps of: mixing one or more phospholipids, the mRNA or DNA, the cell-free translational system, and an aqueous solution into an oily liquid to form a W/O emulsion in which the mRNA or DNA and the cell-free translational system are encapsulated in the phospholipid vesicle; adding an oily liquid containing outer membrane lipids dissolved therein, to an aqueous phase to form a molecular membrane in which the lipids are arranged at the oil/water interface; and adding the W/O emulsion to the oil phase side of the interface and moving the W/O emulsion to the aqueous phase side of the interface such that the outer membrane lipid is added outside of the W/O emulsion to form a liposome.
[claim3]
3. A method for real-time monitoring of intraliposomal protein translation reaction, comprising the step of microscopically observing the liposome after the liposome formation step according to claim 2.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • INOUE TAN
  • SAITO HIROHIDE
  • KATO YUSHO
  • YOSHIKAWA KENICHI
  • YAMADA AYAKO
  • YAMANAKA TORU
IPC(International Patent Classification)
Specified countries (EP2500427)
Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
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