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Method for rendering tissue transparent, reagent for rendering tissue transparent, and tissue observation method NEW

外国特許コード F170009177
整理番号 AF13-04WO
掲載日 2017年9月12日
出願国 アメリカ合衆国
出願番号 201314764060
公報番号 20160011086
出願日 平成25年11月20日(2013.11.20)
公報発行日 平成28年1月14日(2016.1.14)
国際出願番号 JP2013006811
国際公開番号 WO2014115206
国際出願日 平成25年11月20日(2013.11.20)
国際公開日 平成26年7月31日(2014.7.31)
優先権データ
  • 特願2013-012889 (2013.1.28) JP
  • 2013WO-JP04369 (2013.7.17) WO
  • 2013WO-JP06811 (2013.11.20) WO
発明の名称 (英語) Method for rendering tissue transparent, reagent for rendering tissue transparent, and tissue observation method NEW
発明の概要(英語) (US20160011086)
A method for rendering tissue transparent, comprising a procedure of immersing the tissue in a water-soluble solvent comprising 2,2′-thiodiethanol and at least one of glycerol and a nonionic organoiodine compound is provided as a technique capable of sufficiently rendering various organs transparent without causing their changes by a simple operation without using any poisonous or dangerous organic solvent.
In the method for rendering tissue transparent, a mixed solvent of 2,2′-thiodiethanol, glycerol, and a nonionic organoiodine compound aqueous solution is preferably used as the water-soluble solvent.
特許請求の範囲(英語) [claim1]
1. A method for rendering tissue transparent, comprising a procedure of immersing the tissue in a water-soluble solvent comprising 2,2'-thiodiethanol and at least one of glycerol and a nonionic organoiodine compound.
[claim2]
2. The method for rendering tissue transparent according to claim 1, wherein the solvent is a mixed solution of 2,2'-thiodiethanol, glycerol, and a nonionic organoiodine compound aqueous solution wherein proportions by volume of 2,2'-thiodiethanol, glycerol, and a nonionic organoiodine compound aqueous solution having an iodine content of 40% are 10 to 50%, 1 to 20%, and 10 to 70%, respectively.
[claim3]
3. The method for rendering tissue transparent according to claim 1, wherein the solvent is a mixed solution of 2,2'-thiodiethanol and a nonionic organoiodine compound aqueous solution wherein proportions by volume of 2,2'-thiodiethanol and a nonionic organoiodine compound aqueous solution having an iodine content of 40% are 20 to 80% and 80 to 20%, respectively.
[claim4]
4. The method for rendering tissue transparent according to claim 1, wherein the solvent is a mixed solution of 2,2'-thiodiethanol and glycerol wherein proportions by volume of 2,2'-thiodiethanol and glycerol are 70 to 95% and 5 to 30%, respectively.
[claim5]
5. The method for rendering tissue transparent according to claim 1, wherein the solvent further comprises a sucrose aqueous solution.
[claim6]
6. The method for rendering tissue transparent according to claim 1, wherein the solvent has a refractive index of 1.4 to 1.7.
[claim7]
7. The method for rendering tissue transparent according to claim 1, wherein the tissue is one or more selected from the group consisting of the brain, spinal cord, liver, lung, heart, blood vessel, and cancer tissue.
[claim8]
8. The method for rendering tissue transparent according to claim 7, wherein the tissue has a thickness of 1 mm or more.
[claim9]
9. A reagent for rendering tissue transparent, comprising a water-soluble solvent comprising 2,2'-thiodiethanol and at least one of glycerol and a nonionic organoiodine compound.
[claim10]
10. A tissue observation method comprising a transparentization procedure which involves immersing tissue in a water-soluble solvent comprising 2,2'-thiodiethanol and at least one of glycerol and a nonionic organoiodine compound.
[claim11]
11. The tissue observation method according to claim 10, comprising a labeling procedure involving fluorescently labeling the tissue,
the transparentization procedure involving immersing the fluorescently labeled tissue in the solvent, and
a detection procedure involving detecting fluorescence emitted from the fluorescent label in the transparentized tissue.
[claim12]
12. The tissue observation method according to claim 10, comprising the transparentization procedure involving immersing the tissue in the solvent,
a labeling procedure involving fluorescently labeling the transparentized tissue, and
a detection procedure involving detecting fluorescence emitted from the fluorescent label in the transparentized and fluorescently labeled tissue.
[claim13]
13. The tissue observation method according to claim 11, wherein the fluorescence is observed using a fluorescence microscope, a fluorescence stereomicroscope, a confocal microscope, or a multiphoton microscope in the detection procedure.
  • 発明者/出願人(英語)
  • ONODERA HIROSHI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Creation of a Novel Technology towards Diagnosis and Treatment based on Understanding of Molecular Pathogenesis of Psychiatric and Neurological Disorders AREA
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