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Small RNA-dependent translational regulatory system in cell or artificial cell model NEW

外国特許コード F170009178
整理番号 I029P002US1
掲載日 2017年9月12日
出願国 アメリカ合衆国
出願番号 201314021260
公報番号 20140030707
公報番号 9057065
出願日 平成25年9月9日(2013.9.9)
公報発行日 平成26年1月30日(2014.1.30)
公報発行日 平成27年6月16日(2015.6.16)
国際出願番号 JP2008071214
国際公開番号 WO2009066758
国際出願日 平成20年11月21日(2008.11.21)
国際公開日 平成21年5月28日(2009.5.28)
優先権データ
  • 特願2007-303661 (2007.11.22) JP
  • 2008WO-JP71214 (2008.11.21) WO
  • 2010US-12743908 (2010.8.31) US
発明の名称 (英語) Small RNA-dependent translational regulatory system in cell or artificial cell model NEW
発明の概要(英語) (US9057065)
An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein.
The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5′ to the ribosome-binding site, and a nucleotide sequence located 5′ to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3′ to the start codon, and a nucleotide sequence located 3′ to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.
特許請求の範囲(英語) [claim1]
1. An mRNA comprising a microRNA-binding site located 3' to the start codon and a nucleotide sequence located 3' to the microRNA-binding site, the nucleotide sequence encoding a protein, wherein the microRNA-binding site has a sequence complementary to a microRNA selected from the group consisting of miR164, miR170, miR171, miR156 miR159a and miR163.
[claim2]
2. A translation/expression regulation system comprising an mRNA according to claim 1.
[claim3]
3. A liposome comprising an mRNA according to claim 1 encapsulated therein.
[claim4]
4. The liposome according to claim 3, wherein the liposome is produced by the method comprising steps of: mixing one or more phospholipids, the mRNA, a cell-free translational system, and an aqueous solution into an oily liquid to form a W/O emulsion in which the mRNA and the cell-free translational system are encapsulated in the phospholipid vesicle;
adding an oily liquid containing outer membrane lipids dissolved therein, to an aqueous phase to form a molecular membrane in which the lipids are arranged at the oil/water interface; and
adding the W/O emulsion to the oil phase side of the interface and moving the W/O emulsion to the aqueous phase side of the interface such that the outer membrane lipid is added outside of the W/O emulsion to form a liposome.
  • 発明者/出願人(英語)
  • INOUE TAN
  • SAITO HIROHIDE
  • KATO YUSHO
  • YOSHIKAWA KENICHI
  • YAMADA AYAKO
  • YAMANAKA TORU
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
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