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Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method NEW

外国特許コード F170009185
整理番号 K02709WO
掲載日 2017年9月12日
出願国 アメリカ合衆国
出願番号 201314374116
公報番号 20140370496
公報番号 9617606
出願日 平成25年1月24日(2013.1.24)
公報発行日 平成26年12月18日(2014.12.18)
公報発行日 平成29年4月11日(2017.4.11)
国際出願番号 JP2013051389
国際公開番号 WO2013111800
国際出願日 平成25年1月24日(2013.1.24)
国際公開日 平成25年8月1日(2013.8.1)
優先権データ
  • 特願2012-013087 (2012.1.25) JP
  • 2013WO-JP51389 (2013.1.24) WO
発明の名称 (英語) Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method NEW
発明の概要(英語) (US9617606)
The present invention provides an oligonucleotide for HIV detection including a base sequence which is at least 80% identical to a base sequence composed of 10 or more continuous bases in a base sequence represented by SEQ ID NO. 1 or 6, and an HIV detection kit and an HIV detection method that uses the oligonucleotide for HIV detection.
特許請求の範囲(英語) [claim1]
1. An HIV detection kit comprising a polyA polymerase;
an oligonucleotide having a polyT and a base sequence complementary to an adapter sequence on the 5' side of the polyT, wherein the base sequence complementary to the adapter sequence is not complementary to an HIV gene;
a primer set for HIV detection which consists of a forward primer and a reverse primer, wherein the forward primer comprises SEQ ID NO: 5 and the reverse primer consists of the base sequence complementary to the adapter sequence; and
a probe having the base sequence comprising SEQ ID NO: 2, wherein the probe is labelled with a fluorescent dye, a radioisotope, a chemiluminescent body, an enzyme, or an antibody.
[claim2]
2. A method of detecting an HIV by detecting a short RNA produced by HIV-infected cells, wherein the short RNA produced by HIV-infected cells is a complex of transcripts of the HIV-1 mRNA from the transcription initiation site to the nucleotide position 50 to 71 of SEQ ID NO: 1 at which the transcription stops, which method comprises using the HIV detection kit of claim 1 for:
(a) adding a polyA at the 3' end of the short RNA produced by HIV-infected cells in a nucleic acid sample using the polyA polymerase;
(b) synthesizing, from the mRNA, a cDNA complementary to the mRNA by a reverse transcription reaction using the oligonucleotide having a polyT and a base sequence complementary to an adapter sequence on the 5' side of the polyT, wherein the base sequence complementary to the adapter sequence is not complementary to an HIV gene;
(c) amplifying, from the cDNA, a target base sequence having a sequence of a cDNA of HIV by using the primer set for HIV detection of the HIV detection kit wherein the primer set comprises the forward primer comprising SEQ ID NO: 5 and the reverse primer having a base sequence complementary to the adapter sequence; and
(d) detecting an amplification product of the target base sequence using the probe having the base sequence comprising SEQ ID NO: 2 wherein the probe is labelled with a fluorescent dye, a radioisotope, a chemiluminescent body, an enzyme, or an antibody, thereby detecting the HIV.
  • 発明者/出願人(英語)
  • MIZUTANI TAKETOSHI
  • ISHIZAKA AYA
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) PRESTO RNA and biofunctions AREA
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