Top > Search of International Patents > Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method

Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method

Foreign code F170009185
File No. K02709WO
Posted date Sep 12, 2017
Country United States of America
Application number 201314374116
Gazette No. 20140370496
Gazette No. 9617606
Date of filing Jan 24, 2013
Gazette Date Dec 18, 2014
Gazette Date Apr 11, 2017
International application number JP2013051389
International publication number WO2013111800
Date of international filing Jan 24, 2013
Date of international publication Aug 1, 2013
Priority data
  • P2012-013087 (Jan 25, 2012) JP
  • 2013WO-JP51389 (Jan 24, 2013) WO
Title Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method
Abstract (US9617606)
The present invention provides an oligonucleotide for HIV detection including a base sequence which is at least 80% identical to a base sequence composed of 10 or more continuous bases in a base sequence represented by SEQ ID NO. 1 or 6, and an HIV detection kit and an HIV detection method that uses the oligonucleotide for HIV detection.
Scope of claims [claim1]
1. An HIV detection kit comprising a polyA polymerase;
an oligonucleotide having a polyT and a base sequence complementary to an adapter sequence on the 5' side of the polyT, wherein the base sequence complementary to the adapter sequence is not complementary to an HIV gene;
a primer set for HIV detection which consists of a forward primer and a reverse primer, wherein the forward primer comprises SEQ ID NO: 5 and the reverse primer consists of the base sequence complementary to the adapter sequence; and
a probe having the base sequence comprising SEQ ID NO: 2, wherein the probe is labelled with a fluorescent dye, a radioisotope, a chemiluminescent body, an enzyme, or an antibody.
[claim2]
2. A method of detecting an HIV by detecting a short RNA produced by HIV-infected cells, wherein the short RNA produced by HIV-infected cells is a complex of transcripts of the HIV-1 mRNA from the transcription initiation site to the nucleotide position 50 to 71 of SEQ ID NO: 1 at which the transcription stops, which method comprises using the HIV detection kit of claim 1 for:
(a) adding a polyA at the 3' end of the short RNA produced by HIV-infected cells in a nucleic acid sample using the polyA polymerase;
(b) synthesizing, from the mRNA, a cDNA complementary to the mRNA by a reverse transcription reaction using the oligonucleotide having a polyT and a base sequence complementary to an adapter sequence on the 5' side of the polyT, wherein the base sequence complementary to the adapter sequence is not complementary to an HIV gene;
(c) amplifying, from the cDNA, a target base sequence having a sequence of a cDNA of HIV by using the primer set for HIV detection of the HIV detection kit wherein the primer set comprises the forward primer comprising SEQ ID NO: 5 and the reverse primer having a base sequence complementary to the adapter sequence; and
(d) detecting an amplification product of the target base sequence using the probe having the base sequence comprising SEQ ID NO: 2 wherein the probe is labelled with a fluorescent dye, a radioisotope, a chemiluminescent body, an enzyme, or an antibody, thereby detecting the HIV.
  • Inventor, and Inventor/Applicant
  • MIZUTANI TAKETOSHI
  • ISHIZAKA AYA
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO RNA and biofunctions AREA
Please contact us by E-mail or facsimile if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close