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Planar patch clamp device and planar patch clamp system NEW

外国特許コード F170009188
整理番号 AF19-07WO
掲載日 2017年9月12日
出願国 アメリカ合衆国
出願番号 201414915560
公報番号 20160209395
出願日 平成26年8月29日(2014.8.29)
公報発行日 平成28年7月21日(2016.7.21)
国際出願番号 JP2014072808
国際公開番号 WO2015030201
国際出願日 平成26年8月29日(2014.8.29)
国際公開日 平成27年3月5日(2015.3.5)
優先権データ
  • 特願2013-180684 (2013.8.30) JP
  • 2014WO-JP72808 (2014.8.29) WO
発明の名称 (英語) Planar patch clamp device and planar patch clamp system NEW
発明の概要(英語) (US20160209395)
A planar patch clamp device comprising: an electrical insulating substrate (2) having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole (3) in the cell arrangement region which does not pass cells, but pass liquid; a first reservoir (6) provided at the first surface side (2S) of the electrical insulating substrate (2) to be able to communicate with the through hole (3) and hold a first conductive liquid; a first electrode part (7) arranged to be able to be electrically conductive with the first reservoir (6) through the first conductive liquid; a second reservoir (6′) provided at the second surface side (2S′) of the electrical insulating substrate to be able to communicate with the through hole (3) and hold a second conductive liquid; a second electrode part (7′) arranged to be able to be electrically conductive with the second reservoir (6′) through the second conductive liquid; a supply path (8) connected to the second reservoir (6′) and supplying the second conductive liquid to the second reservoir; a discharge path (9) connected to the second reservoir and discharging the second conductive liquid from the second reservoir (6′); and a valve (10) provided in the supply path and/or discharge path, able to allow or stop the flow of the second conductive liquid, and also able to allow or stop electrical conduction between the second reservoir (6′) and the second electrode part (7′).
特許請求の範囲(英語) [claim1]
1. A planar patch clamp device comprising: an electrical insulating substrate having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole in the cell arrangement region which does not pass cells, but pass liquid;
a first reservoir provided at the first surface side of the electrical insulating substrate to be able to communicate with the through hole and hold a first conductive liquid;
a first electrode part arranged to be able to be electrically conductive with the first reservoir through the first conductive liquid;
a second reservoir provided at the second surface side of the electrical insulating substrate to be able to communicate with the through hole and hold a second conductive liquid;
a second electrode part arranged to be able to be electrically conductive with the second reservoir through the second conductive liquid;
a supply path connected to the second reservoir and supplying the second conductive liquid to the second reservoir;
a discharge path connected to the second reservoir and discharging the second conductive liquid from the second reservoir; and
a valve provided in the supply path and/or discharge path, able to allow or stop the flow of the second conductive liquid, and also able to allow or stop electrical conduction between the second reservoir and the second electrode part,
wherein
the electrical insulating substrate has a plurality of cell arrangement regions and corresponding through holes and is provided with a plurality of second reservoirs corresponding to the plurality of cell arrangement regions;
the supply path has a main supply path and a plurality of branch supply paths respectively branched from the main supply path and connected to the plurality of second reservoirs;
the discharge path has a plurality of branch discharge paths respectively connected to the plurality of the second reservoirs and a main discharge path where the plurality of branch discharge paths merge;
the second electrode part is provided at the main supply path and/or main discharge path; and
a valve is provided at each branch supply path and/or each branch discharge path.
[claim2]
2. (canceled)
[claim3]
3. The planar patch clamp device according to claim 1, wherein an electric resistance before and after the valve is 1 megaohm or more at the time of valve closing.
[claim4]
4. The planar patch clamp device according to claim 1, wherein the planar patch clamp device is a culture-type planar patch clamp device.
[claim5]
5. The planar patch clamp device according to claim 4, wherein an extracellular matrix-forming material is deposited at the rims of the through holes on the first surface of the substrate.
[claim6]
6. A planar patch clamp system, comprising: the planar patch clamp device according to claim 1;
a control part controlling opening and closing each valve of said planar patch clamp device; and
an electrical detecting part detecting an electrical signal at each electrode part of said planar patch clamp device.
[claim7]
7. The planar patch clamp system according to claim 6, comprising a plurality of said planar patch clamp devices.
[claim8]
8. The planar patch clamp system according to claim 6, further comprising an optical detecting part for detecting an optical signal derived from a cell arranged on said planar patch clamp device.
[claim9]
9. A method for screening drugs having an effect on a nerve cell network by using the planar patch clamp device according to claim 1.
[claim10]
10. A method for screening drugs having an effect on a nerve cell network by using the planar patch clamp system according to claim 6.
  • 発明者/出願人(英語)
  • URISU TSUNEO
  • WANG ZHI-HONG
  • UNO HIDETAKA
  • NAGAOKA YASUTAKA
  • KOBAYASHI KEI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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