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Planar patch clamp device and planar patch clamp system

Foreign code F170009188
File No. AF19-07WO
Posted date Sep 12, 2017
Country United States of America
Application number 201414915560
Gazette No. 20160209395
Date of filing Aug 29, 2014
Gazette Date Jul 21, 2016
International application number JP2014072808
International publication number WO2015030201
Date of international filing Aug 29, 2014
Date of international publication Mar 5, 2015
Priority data
  • P2013-180684 (Aug 30, 2013) JP
  • 2014WO-JP72808 (Aug 29, 2014) WO
Title Planar patch clamp device and planar patch clamp system
Abstract (US20160209395)
A planar patch clamp device comprising: an electrical insulating substrate (2) having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole (3) in the cell arrangement region which does not pass cells, but pass liquid; a first reservoir (6) provided at the first surface side (2S) of the electrical insulating substrate (2) to be able to communicate with the through hole (3) and hold a first conductive liquid; a first electrode part (7) arranged to be able to be electrically conductive with the first reservoir (6) through the first conductive liquid; a second reservoir (6′) provided at the second surface side (2S′) of the electrical insulating substrate to be able to communicate with the through hole (3) and hold a second conductive liquid; a second electrode part (7′) arranged to be able to be electrically conductive with the second reservoir (6′) through the second conductive liquid; a supply path (8) connected to the second reservoir (6′) and supplying the second conductive liquid to the second reservoir; a discharge path (9) connected to the second reservoir and discharging the second conductive liquid from the second reservoir (6′); and a valve (10) provided in the supply path and/or discharge path, able to allow or stop the flow of the second conductive liquid, and also able to allow or stop electrical conduction between the second reservoir (6′) and the second electrode part (7′).
Scope of claims [claim1]
1. A planar patch clamp device comprising: an electrical insulating substrate having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole in the cell arrangement region which does not pass cells, but pass liquid;
a first reservoir provided at the first surface side of the electrical insulating substrate to be able to communicate with the through hole and hold a first conductive liquid;
a first electrode part arranged to be able to be electrically conductive with the first reservoir through the first conductive liquid;
a second reservoir provided at the second surface side of the electrical insulating substrate to be able to communicate with the through hole and hold a second conductive liquid;
a second electrode part arranged to be able to be electrically conductive with the second reservoir through the second conductive liquid;
a supply path connected to the second reservoir and supplying the second conductive liquid to the second reservoir;
a discharge path connected to the second reservoir and discharging the second conductive liquid from the second reservoir; and
a valve provided in the supply path and/or discharge path, able to allow or stop the flow of the second conductive liquid, and also able to allow or stop electrical conduction between the second reservoir and the second electrode part,
wherein
the electrical insulating substrate has a plurality of cell arrangement regions and corresponding through holes and is provided with a plurality of second reservoirs corresponding to the plurality of cell arrangement regions;
the supply path has a main supply path and a plurality of branch supply paths respectively branched from the main supply path and connected to the plurality of second reservoirs;
the discharge path has a plurality of branch discharge paths respectively connected to the plurality of the second reservoirs and a main discharge path where the plurality of branch discharge paths merge;
the second electrode part is provided at the main supply path and/or main discharge path; and
a valve is provided at each branch supply path and/or each branch discharge path.
[claim2]
2. (canceled)
[claim3]
3. The planar patch clamp device according to claim 1, wherein an electric resistance before and after the valve is 1 megaohm or more at the time of valve closing.
[claim4]
4. The planar patch clamp device according to claim 1, wherein the planar patch clamp device is a culture-type planar patch clamp device.
[claim5]
5. The planar patch clamp device according to claim 4, wherein an extracellular matrix-forming material is deposited at the rims of the through holes on the first surface of the substrate.
[claim6]
6. A planar patch clamp system, comprising: the planar patch clamp device according to claim 1;
a control part controlling opening and closing each valve of said planar patch clamp device; and
an electrical detecting part detecting an electrical signal at each electrode part of said planar patch clamp device.
[claim7]
7. The planar patch clamp system according to claim 6, comprising a plurality of said planar patch clamp devices.
[claim8]
8. The planar patch clamp system according to claim 6, further comprising an optical detecting part for detecting an optical signal derived from a cell arranged on said planar patch clamp device.
[claim9]
9. A method for screening drugs having an effect on a nerve cell network by using the planar patch clamp device according to claim 1.
[claim10]
10. A method for screening drugs having an effect on a nerve cell network by using the planar patch clamp system according to claim 6.
  • Inventor, and Inventor/Applicant
  • URISU TSUNEO
  • WANG ZHI-HONG
  • UNO HIDETAKA
  • NAGAOKA YASUTAKA
  • KOBAYASHI KEI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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