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Oligonucleotide for hiv detection, hiv detection kit, and hiv detection method NEW

外国特許コード F170009206
整理番号 K02709WO
掲載日 2017年9月12日
出願国 欧州特許庁(EPO)
出願番号 13740936
公報番号 2808387
公報番号 2808387
出願日 平成25年1月24日(2013.1.24)
公報発行日 平成26年12月3日(2014.12.3)
公報発行日 平成29年6月21日(2017.6.21)
国際出願番号 JP2013051389
国際公開番号 WO2013111800
国際出願日 平成25年1月24日(2013.1.24)
国際公開日 平成25年8月1日(2013.8.1)
優先権データ
  • 特願2012-013087 (2012.1.25) JP
  • 2013WO-JP51389 (2013.1.24) WO
発明の名称 (英語) Oligonucleotide for hiv detection, hiv detection kit, and hiv detection method NEW
発明の概要(英語) (EP2808387)
The present invention provides an oligonucleotide for HIV detection including a base sequence which is at least 80% identical to a base sequence composed of 10 or more continuous bases in a base sequence represented by SEQ ID NO. 1 or 6, and an HIV detection kit and an HIV detection method that uses the oligonucleotide for HIV detection.
特許請求の範囲(英語) [claim1]
1. An oligonucleotide for HIV detection comprising a base sequence which is at least 80% identical to a base sequence composed of 10 or more continuous bases in a base sequence represented by SEQ ID NO. 1 or 6.
[claim2]
2. The oligonucleotide for HIV detection according to Claim 1, comprising a base sequence which is being at least 90% identical to a base sequence composed of 10 or more continuous bases in the base sequence represented by SEQ ID NO. 1 or 6.
[claim3]
3. The oligonucleotide for HIV detection according to Claim 1, comprising a base sequence which is being at least 95% identical to a base sequence composed of 10 or more continuous bases in the base sequence represented by SEQ ID NO. 1 or 6.
[claim4]
4. The oligonucleotide for HIV detection according to Claim 1, comprising a base sequence which is being at least 98% identical to a base sequence composed of 10 or more continuous bases in the base sequence represented by SEQ ID NO. 1 or 6.
[claim5]
5. The oligonucleotide for HIV detection according to Claim 1, comprising a base sequence composed of 10 or more continuous bases in the base sequence represented by SEQ ID NO. 1 or 6.
[claim6]
6. An HIV detection kit comprising the oligonucleotide for HIV detection according to any one of Claims 1 to 5.
[claim7]
7. An HIV detection method comprising using the oligonucleotide for HIV detection or the HIV detection kit according to any one of Claims 1 to 6.
[claim8]
8. An HIV detection method comprising: (a) a step of adding a polyA at the 3' end of an mRNA in a nucleic acid sample; (b) a step of synthesizing, from the mRNA, a cDNA complementary to the mRNA by a reverse transcription reaction using a polyT and an oligonucleotide having a base sequence complementary to an adapter sequence on the 5' side of the polyT; (c) a step of amplifying, from the cDNA, a target base sequence having a sequence of a cDNA of HIV by using the oligonucleotide for HIV detection according to any one of Claims 1 to 5 and an oligonucleotide having a base sequence complementary to the adapter sequence; and (d) a step of detecting an amplification product of the target base sequence.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • MIZUTANI TAKETOSHI
  • ISHIZAKA AYA
国際特許分類(IPC)
指定国 (EP2808387)
Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
参考情報 (研究プロジェクト等) PRESTO RNA and biofunctions AREA
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