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Oligonucleotide for hiv detection, hiv detection kit, and hiv detection method

Foreign code F170009206
File No. K02709WO
Posted date Sep 12, 2017
Country EPO
Application number 13740936
Gazette No. 2808387
Gazette No. 2808387
Date of filing Jan 24, 2013
Gazette Date Dec 3, 2014
Gazette Date Jun 21, 2017
International application number JP2013051389
International publication number WO2013111800
Date of international filing Jan 24, 2013
Date of international publication Aug 1, 2013
Priority data
  • P2012-013087 (Jan 25, 2012) JP
  • 2013WO-JP51389 (Jan 24, 2013) WO
Title Oligonucleotide for hiv detection, hiv detection kit, and hiv detection method
Abstract (EP2808387)
The present invention provides an oligonucleotide for HIV detection including a base sequence which is at least 80% identical to a base sequence composed of 10 or more continuous bases in a base sequence represented by SEQ ID NO. 1 or 6, and an HIV detection kit and an HIV detection method that uses the oligonucleotide for HIV detection.
Scope of claims [claim1]
1. An oligonucleotide for HIV detection comprising a base sequence which is at least 80% identical to a base sequence composed of 10 or more continuous bases in a base sequence represented by SEQ ID NO. 1 or 6.
[claim2]
2. The oligonucleotide for HIV detection according to Claim 1, comprising a base sequence which is being at least 90% identical to a base sequence composed of 10 or more continuous bases in the base sequence represented by SEQ ID NO. 1 or 6.
[claim3]
3. The oligonucleotide for HIV detection according to Claim 1, comprising a base sequence which is being at least 95% identical to a base sequence composed of 10 or more continuous bases in the base sequence represented by SEQ ID NO. 1 or 6.
[claim4]
4. The oligonucleotide for HIV detection according to Claim 1, comprising a base sequence which is being at least 98% identical to a base sequence composed of 10 or more continuous bases in the base sequence represented by SEQ ID NO. 1 or 6.
[claim5]
5. The oligonucleotide for HIV detection according to Claim 1, comprising a base sequence composed of 10 or more continuous bases in the base sequence represented by SEQ ID NO. 1 or 6.
[claim6]
6. An HIV detection kit comprising the oligonucleotide for HIV detection according to any one of Claims 1 to 5.
[claim7]
7. An HIV detection method comprising using the oligonucleotide for HIV detection or the HIV detection kit according to any one of Claims 1 to 6.
[claim8]
8. An HIV detection method comprising: (a) a step of adding a polyA at the 3' end of an mRNA in a nucleic acid sample; (b) a step of synthesizing, from the mRNA, a cDNA complementary to the mRNA by a reverse transcription reaction using a polyT and an oligonucleotide having a base sequence complementary to an adapter sequence on the 5' side of the polyT; (c) a step of amplifying, from the cDNA, a target base sequence having a sequence of a cDNA of HIV by using the oligonucleotide for HIV detection according to any one of Claims 1 to 5 and an oligonucleotide having a base sequence complementary to the adapter sequence; and (d) a step of detecting an amplification product of the target base sequence.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • MIZUTANI TAKETOSHI
  • ISHIZAKA AYA
IPC(International Patent Classification)
Specified countries (EP2808387)
Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Reference ( R and D project ) PRESTO RNA and biofunctions AREA
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