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Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method NEW

外国特許コード F170009215
整理番号 K02709WO
掲載日 2017年9月13日
出願国 中華人民共和国
出願番号 201380006301
公報番号 104066842
出願日 平成25年1月24日(2013.1.24)
公報発行日 平成26年9月24日(2014.9.24)
国際出願番号 JP2013051389
国際公開番号 WO2013111800
国際出願日 平成25年1月24日(2013.1.24)
国際公開日 平成25年8月1日(2013.8.1)
優先権データ
  • 特願2012-013087 (2012.1.25) JP
  • 2013WO-JP51389 (2013.1.24) WO
発明の名称 (英語) Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method NEW
発明の概要(英語) (CN104066842)
The present invention provides an oligonucleotide for HIV detection, and an HIV detection kit and an HIV detection method that use the oligonucleotide for HIV detection, said oligonucleotide for HIV detection being characterized by having a base sequence comprising at least ten contiguous bases in a base sequence represented by SEQ ID NO: 1 or 6, and having an 80% identity or higher.
特許請求の範囲(英語) [claim1]
1. A detection oligonucleotide HIV, characterized in, that the 6 nucleotide sequence of SEQ ID NO 1 or 10 or more consecutive bases of a nucleotide sequence having 80% or more homology.
[claim2]
2. HIV detector oligonucleotide according to claim 1, characterized in, that the nucleotide sequence of SEQ ID NO 1 or 6 of sequence homologous to 10 of a nucleotide sequence having 90% or more homology or more.
[claim3]
3. HIV detector oligonucleotide according to claim 1, characterized in, SEQ ID No. 1 or 6 with the base sequence of 10 or more consecutive bases of a nucleotide sequence having 95% or more homology.
[claim4]
4. HIV detector oligonucleotide according to claim 1, characterized in, 1 or 6 with the nucleotide sequence of SEQ ID NO 10 or more consecutive bases of a nucleotide sequence having a homology of 98% or more.
[claim5]
5. HIV detector oligonucleotide according to claim 1, having 1 or 6 shown in SEQ ID no 10 or more contiguous bases of nucleotide sequence comprising the nucleotide sequence.
[claim6]
6. A kit for detecting HIV, characterized in, containing claim 1-5, HIV any one of the detector oligonucleotide.
[claim7]
7. A method for detecting HIV, characterized in, claim 1-6 use, according to any one oligonucleotide for detection or detection kit HIV HIV.
[claim8]
8. A method for detecting HIV, characterized in, comprising the steps of:
(A) a nucleic acid in a sample mRNA 3 'terminus polyA step;
(B) with polyT polyT of the and a 5' side of the base sequence complementary to a linker sequence, a reverse transcription reaction with the complementary cDNA mRNA mRNA by the synthesis step;
(C) using claim 1-5, HIV any one linker sequence and an oligonucleotide having complementary detection oligonucleotide nucleotide sequence, the target base sequence by cDNA HIV cDNA of the amplified sequence with step; and
(D) the step of detecting target sequence amplification product.
  • 出願人(英語)
  • HTC
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • WU CHIH-HSIANG
  • MIZUTANI TAKETOSHI
  • ISHIZAKA AYA
国際特許分類(IPC)
参考情報 (研究プロジェクト等) PRESTO RNA and biofunctions AREA
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