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Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method

Foreign code F170009215
File No. K02709WO
Posted date Sep 13, 2017
Country China
Application number 201380006301
Gazette No. 104066842
Date of filing Jan 24, 2013
Gazette Date Sep 24, 2014
International application number JP2013051389
International publication number WO2013111800
Date of international filing Jan 24, 2013
Date of international publication Aug 1, 2013
Priority data
  • P2012-013087 (Jan 25, 2012) JP
  • 2013WO-JP51389 (Jan 24, 2013) WO
Title Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method
Abstract (CN104066842)
The present invention provides an oligonucleotide for HIV detection, and an HIV detection kit and an HIV detection method that use the oligonucleotide for HIV detection, said oligonucleotide for HIV detection being characterized by having a base sequence comprising at least ten contiguous bases in a base sequence represented by SEQ ID NO: 1 or 6, and having an 80% identity or higher.
Scope of claims [claim1]
1. A detection oligonucleotide HIV, characterized in, that the 6 nucleotide sequence of SEQ ID NO 1 or 10 or more consecutive bases of a nucleotide sequence having 80% or more homology.
[claim2]
2. HIV detector oligonucleotide according to claim 1, characterized in, that the nucleotide sequence of SEQ ID NO 1 or 6 of sequence homologous to 10 of a nucleotide sequence having 90% or more homology or more.
[claim3]
3. HIV detector oligonucleotide according to claim 1, characterized in, SEQ ID No. 1 or 6 with the base sequence of 10 or more consecutive bases of a nucleotide sequence having 95% or more homology.
[claim4]
4. HIV detector oligonucleotide according to claim 1, characterized in, 1 or 6 with the nucleotide sequence of SEQ ID NO 10 or more consecutive bases of a nucleotide sequence having a homology of 98% or more.
[claim5]
5. HIV detector oligonucleotide according to claim 1, having 1 or 6 shown in SEQ ID no 10 or more contiguous bases of nucleotide sequence comprising the nucleotide sequence.
[claim6]
6. A kit for detecting HIV, characterized in, containing claim 1-5, HIV any one of the detector oligonucleotide.
[claim7]
7. A method for detecting HIV, characterized in, claim 1-6 use, according to any one oligonucleotide for detection or detection kit HIV HIV.
[claim8]
8. A method for detecting HIV, characterized in, comprising the steps of:
(A) a nucleic acid in a sample mRNA 3 'terminus polyA step;
(B) with polyT polyT of the and a 5' side of the base sequence complementary to a linker sequence, a reverse transcription reaction with the complementary cDNA mRNA mRNA by the synthesis step;
(C) using claim 1-5, HIV any one linker sequence and an oligonucleotide having complementary detection oligonucleotide nucleotide sequence, the target base sequence by cDNA HIV cDNA of the amplified sequence with step; and
(D) the step of detecting target sequence amplification product.
  • Applicant
  • HTC
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • WU CHIH-HSIANG
  • MIZUTANI TAKETOSHI
  • ISHIZAKA AYA
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO RNA and biofunctions AREA
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