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Planar patch clamp device and planar patch clamp system

Foreign code F170009218
File No. AF19-07WO
Posted date Sep 13, 2017
Country China
Application number 201480048002
Gazette No. 105555945
Date of filing Aug 29, 2014
Gazette Date May 4, 2016
International application number JP2014072808
International publication number WO2015030201
Date of international filing Aug 29, 2014
Date of international publication Mar 5, 2015
Priority data
  • P2013-180684 (Aug 30, 2013) JP
  • 2014WO-JP72808 (Aug 29, 2014) WO
Title Planar patch clamp device and planar patch clamp system
Abstract (CN105555945)
A planar patch clamp device comprising: an electrically non-conductive substrate (2) having a through-hole (3) which passes liquid yet does not pass cells in a cell arrangement region (4); a first liquid reservoir unit (6) for holding a first electroconductive liquid, the first liquid reservoir unit (6) being provided so as to allow communication with the through-hole (3) on a first surface (2S) of the substrate (2); a first electrode unit (7) arranged so as to allow electrical conductivity with the first liquid reservoir unit (6); a second liquid reservoir unit (6') for holding a second electroconductive liquid, the second liquid reservoir unit (6) being provided so as to allow communication with the through-hole (3) on a second surface (2S') of the substrate (2); a second electrode unit (7') arranged so as to allow electrical conductivity with the first liquid reservoir unit (6); a liquid feed channel (8) for feeding the second electroconductive liquid to the second liquid reservoir unit (6'); a liquid discharge channel (9) for discharging the second electroconductive liquid from the second liquid reservoir unit (6'); and a valve (10) disposed in the liquid feed channel (8) and/or the liquid discharge channel (9), the valve (10) being capable of permitting or stopping the distribution of the second electroconductive liquid, and permitting or stopping electrical conductivity with the second liquid reservoir unit (6') and the second electrode unit (7').
Scope of claims [claim1]
1. A planar patch clamp device, having:
An electrically insulating substrate, which includes the 1st surface and as the 1st cell placement surface region of an opposite surface from 2nd surface, and electrically insulating substrate through the cells in the cell arrangement area having a non-liquid but can be made through a through hole;
1st sump portion, wherein the through-hole provided in communicating manner so as to be in an electrically insulating substrate with a 1st surface side, and serves to maintain 1st conductive liquid;
1st electrode unit, configured to be capable device supplying electric conduction to the 1st through the 1st conductive liquid;
2nd sump portion, wherein the through-hole provided in communicating manner so as to be in an electrically insulating substrate with a 2nd surface side, 2nd and serves to maintain the conductive liquid;
2nd electrode unit, configured to be capable 2nd conductive liquid via electrical communication with the 2nd liquid storage portion;
Send flow path, which is connected with the sump portion 2nd, 2nd conductive liquid delivery reservoir and to 2nd;
A drainage flow path, which is connected with the sump portion 2nd, 2nd 2nd conductive liquid discharged from the reservoir; and
Valve, and/or send flow path which is disposed in the drainage flow path, it is possible to allow or stop the flow of liquid conductive 2nd, 2nd electrode portion with the reservoir and can allow or stop the 2nd electrical conduction.
[claim2]
2. The planar patch clamp device according to claim 1, wherein,
An electrically insulating substrate and respectively having a plurality of cell arrangement area with a plurality of cell arrangement area corresponding through hole, and electrically insulating substrate with a plurality of cells provided in correspondence with a plurality of 2nd reservoir mounting region,
Send flow path having: feeding the main passage, and from the liquid feed main flow path is branched and connected respectively to the plurality of 2nd device supplying a plurality of liquid-feeding flow paths,
A drainage flow path having: a plurality of connected respectively to the plurality of 2nd reservoir drainage branch flow path, and a plurality of drainage tributary drainage sum of the main passage,
2nd electrode portion is provided in the main flow path and/or drain liquid prepared by the main passage,
Each valve is provided in and/or the drainage liquid feeding branch flow path branch flow path.
[claim3]
3. The planar patch clamp device according to claim 1 or 2, wherein,
Has a value of 1 before and after a valve when the valve closes more than mega ohms.
[claim4]
4. A planar patch clamp system, comprising:
Claim 1 to 3 according to any one of the planar patch clamp device;
The control plane of opening and closing of the valves of the patch-clamp device control unit; and
Planar patch clamp device according to the detection electrode portion of an electrical signal-charge detection unit.
[claim5]
5. The planar patch clamp system according to claim 4, wherein,
The planar patch clamp system includes a plurality of patch-clamp device.
[claim6]
6. The planar patch clamp system according to claim 4 or 5, wherein,
The planar patch clamp system further includes a light detector, light detector detecting light from the patch-clamp device disposed in a plane of cells in light signal.
[claim7]
7. One method, the use of planar patch clamp device as claimed in any claim 1 to 3, or according to any one of claim 4 to 7 planar patch clamp system, for screening an agent for the neural network.
  • Applicant
  • LIEBHERR
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • ANDREAS ORLEAN
  • URISU TSUNEO
  • WANG ZHI-HONG
  • UNO HIDETAKA
  • NAGAOKA YASUTAKA
  • KOBAYASHI KEI
IPC(International Patent Classification)
Reference ( R and D project ) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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