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Kit for sealing beads and detecting target molecule NEW

外国特許コード F170009221
整理番号 AF19-01US4
掲載日 2017年9月13日
出願国 アメリカ合衆国
出願番号 201615351522
公報番号 20170115284
出願日 平成28年11月15日(2016.11.15)
公報発行日 平成29年4月27日(2017.4.27)
国際出願番号 JP2012055884
国際公開番号 WO2012121310
国際出願日 平成24年3月7日(2012.3.7)
国際公開日 平成24年9月13日(2012.9.13)
優先権データ
  • 特願2011-050629 (2011.3.8) JP
  • 2012WO-JP55884 (2012.3.7) WO
  • 2013US-14003509 (2013.9.6) US
  • 2016US-15082195 (2016.3.28) US
発明の名称 (英語) Kit for sealing beads and detecting target molecule NEW
発明の概要(英語) (US20170115284)
This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity.
This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
特許請求の範囲(英語) [claim1]

1-12. (canceled)
[claim2]
13. A kit comprising: an array including: a lower layer section provided with a plurality of receptacles being separated from each other by a side wall having a hydrophobic upper surface; and
an upper layer section facing, via a space, a surface of the lower layer section on which surface the plurality of receptacles are provided;
beads,
a hydrophilic solvent; and
a hydrophobic solvent;
each of the plurality of receptacles being capable of storing only one of the beads, and
said kit being used to carry out a method for sealing the beads, the method comprising the steps of:
(i) introducing the hydrophilic solvent containing the beads into the space between the lower layer section and the upper layer section; and
(ii) introducing the hydrophobic solvent into the space, step (ii) being carried out after step (i).
[claim3]
14. The kit as set forth in claim 13, wherein: each of the plurality of receptacles has a hydrophilic bottom surface.
[claim4]
15. The kit as set forth in claim 13, wherein: the upper layer section has a hydrophobic surface facing the lower layer section.
[claim5]
16. The kit as set forth in claim 14, wherein: the upper layer section has a hydrophobic surface facing the lower layer section.
[claim6]
17. The kit as set forth in claim 13, wherein: at least one of the upper layer section and the lower layer section has a through-hole via which a fluid is introduced into the space.
[claim7]
18. The kit as set forth in claim 14, wherein: at least one of the upper layer section and the lower layer section has a through-hole via which a fluid is introduced into the space.
[claim8]
19. The kit as set forth in claim 15, wherein: at least one of the upper layer section and the lower layer section has a through-hole via which a fluid is introduced into the space.
[claim9]
20. The kit as set forth in claim 16, wherein: at least one of the upper layer section and the lower layer section has a through-hole via which a fluid is introduced into the space.
  • 発明者/出願人(英語)
  • NOJI HIROYUKI
  • IINO RYOTA
  • ARAKI SUGURU
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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