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Kit for sealing beads and detecting target molecule

Foreign code F170009221
File No. AF19-01US4
Posted date Sep 13, 2017
Country United States of America
Application number 201615351522
Gazette No. 20170115284
Date of filing Nov 15, 2016
Gazette Date Apr 27, 2017
International application number JP2012055884
International publication number WO2012121310
Date of international filing Mar 7, 2012
Date of international publication Sep 13, 2012
Priority data
  • P2011-050629 (Mar 8, 2011) JP
  • 2012WO-JP55884 (Mar 7, 2012) WO
  • 2013US-14003509 (Sep 6, 2013) US
  • 2016US-15082195 (Mar 28, 2016) US
Title Kit for sealing beads and detecting target molecule
Abstract (US20170115284)
This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity.
This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
Scope of claims [claim1]

1-12. (canceled)
[claim2]
13. A kit comprising: an array including: a lower layer section provided with a plurality of receptacles being separated from each other by a side wall having a hydrophobic upper surface; and
an upper layer section facing, via a space, a surface of the lower layer section on which surface the plurality of receptacles are provided;
beads,
a hydrophilic solvent; and
a hydrophobic solvent;
each of the plurality of receptacles being capable of storing only one of the beads, and
said kit being used to carry out a method for sealing the beads, the method comprising the steps of:
(i) introducing the hydrophilic solvent containing the beads into the space between the lower layer section and the upper layer section; and
(ii) introducing the hydrophobic solvent into the space, step (ii) being carried out after step (i).
[claim3]
14. The kit as set forth in claim 13, wherein: each of the plurality of receptacles has a hydrophilic bottom surface.
[claim4]
15. The kit as set forth in claim 13, wherein: the upper layer section has a hydrophobic surface facing the lower layer section.
[claim5]
16. The kit as set forth in claim 14, wherein: the upper layer section has a hydrophobic surface facing the lower layer section.
[claim6]
17. The kit as set forth in claim 13, wherein: at least one of the upper layer section and the lower layer section has a through-hole via which a fluid is introduced into the space.
[claim7]
18. The kit as set forth in claim 14, wherein: at least one of the upper layer section and the lower layer section has a through-hole via which a fluid is introduced into the space.
[claim8]
19. The kit as set forth in claim 15, wherein: at least one of the upper layer section and the lower layer section has a through-hole via which a fluid is introduced into the space.
[claim9]
20. The kit as set forth in claim 16, wherein: at least one of the upper layer section and the lower layer section has a through-hole via which a fluid is introduced into the space.
  • Inventor, and Inventor/Applicant
  • NOJI HIROYUKI
  • IINO RYOTA
  • ARAKI SUGURU
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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