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NOVEL MICROORGANISM AND METHOD FOR CONSTRUCTING SAME NEW 新技術説明会

外国特許コード F170009246
整理番号 (15079)
掲載日 2017年10月4日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP009268
国際公開番号 WO 2017159499
国際出願日 平成29年3月8日(2017.3.8)
国際公開日 平成29年9月21日(2017.9.21)
優先権データ
  • 特願2016-052879 (2016.3.16) JP
発明の名称 (英語) NOVEL MICROORGANISM AND METHOD FOR CONSTRUCTING SAME NEW 新技術説明会
発明の概要(英語) A novel microorganism, which is a phosphorus-dissolving bacterium belonging to the genus Pseudomonas and decomposing a hardly soluble phosphorus compound into phosphoric acid, is a mutant defective in the function of PP_4484 protein.
特許請求の範囲(英語) [claim1]
1. It is the Pseudomonas being attached phosphorus melting germ which disassembles the slightly soluble phosphorus compound to phosphoric acid, function of the PP-4484 protein it is the mutant which is deficient, the new microbe.
[claim2]
2. The aforementioned mutant is the transposon mutant, in claim 1 the new microbe of statement.
[claim3]
3. It is the phosphorus melting germ which disassembles the slightly soluble phosphorus compound to phosphoric acid, the Pseudomonas sp.
MT-5 stocks which possess bio film compilation ability (trust number NITE BP-02215) it is, the new microbe.
[claim4]
4. Either of the claim 1-3 it made the bio film which includes the new microbe of statement in 1 sections the surface come in contact, the seed.
[claim5]
5. Introducing mutation into the Pseudomonas being attached phosphorus melting germ which is disassembled, to the phosphoric acid of the slightly soluble phosphorus compound, the process which obtains variant and,
Among the aforementioned variants, bio film formation ability it had with the process which the stocks which are higher than protoroph the screening is done, compilation method of the new microbe.
[claim6]
6. The stocks where function of the PP-4484 protein is deficient in the process which the aforementioned screening is done, are selected, in claim 5 compilation method of the new microbe of statement.
[claim7]
7. The aforementioned phosphorus melting germ, is Pseudomonas putida, in claim 5 or 6 compilation method of the new microbe of statement.
[claim8]
8. The aforementioned phosphorus melting germ, Pseudomonas sp.
P-451 stocks (trust number NITE BP-02205) is, in claim 5 or 6 compilation method of the new microbe of statement.
[claim9]
9. In the process which introduces the aforementioned mutation, as the transposon donor transposon mutation is introduced making use of E.coli HB101/pRK2013 making use of E.coli MC4100/pMAR2xT7, as the transposon helper, either of the claim 5-8 in 1 sections compilation method of the new microbe of statement.
[claim10]
10. Either of the claim 5-9 in 1 sections with the seed, culturing the new microbe which was drawn up with the compilation method of the new microbe of statement at temperature above the 20.deg.C and below the 35.deg.C, it makes the bio film the surface of the seed form, compilation method of the bio film coating seed.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • HIROSHIMA UNIVERSITY
  • 発明者(英語)
  • UEDA AKIHIRO
国際特許分類(IPC)
指定国 (WO2017159499)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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