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MICROSCOPE AND METHOD FOR OBSERVING BIOLOGICAL SAMPLE IN LIVING STATE

外国特許コード F170009256
整理番号 (ST01P002)
掲載日 2017年10月18日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP011837
国際公開番号 WO 2017164332
国際出願日 平成29年3月23日(2017.3.23)
国際公開日 平成29年9月28日(2017.9.28)
優先権データ
  • 特願2016-058946 (2016.3.23) JP
発明の名称 (英語) MICROSCOPE AND METHOD FOR OBSERVING BIOLOGICAL SAMPLE IN LIVING STATE
発明の概要(英語) To observe a biological sample that contains a chemiluminescent substance producing chemiluminescence in a living state using a microscope. The microscope is provided with a light source for emitting control light for varying the state of the chemiluminescence, a demarcation unit for demarcating the irradiation pattern of the control light irradiating an observation surface of the biological sample, and a detector for detecting the chemiluminescence emitted from the biological sample.
特許請求の範囲(英語) [claim1]
1. Being the microscope in order to observe the organism sample in existence state,
As for the aforementioned organism sample, implication the chemiluminescence substance which forms chemiluminescence,
As for the aforementioned microscope,
The illuminant which ejects the control light which changes the state of the aforementioned chemiluminescence and,
The demarcation section which demarcates the lighting pattern of the aforementioned control light which is irradiated to the observation aspect of the aforementioned organism sample and,
The detector which detects the aforementioned chemiluminescence which is begun shooting from the aforementioned organism sample and,
The microscope which features that it has.
[claim2]
2. The aforementioned control light controls the formation of the aforementioned chemiluminescence with the lighting,
As for the aforementioned demarcation section, in order to the 1st territory which should detect the inside aforementioned chemiluminescence of the observation aspect of the aforementioned organism sample without irradiating the aforementioned control light, to irradiate the aforementioned control light to the 2nd territory which surrounds the aforementioned 1st territory, in the claim 1 which features that lighting pattern of the aforementioned control light is demarcated the microscope of statement.
[claim3]
3. The aforementioned control light promotes the formation of the aforementioned chemiluminescence with the lighting,
As for the aforementioned demarcation section, in order to irradiate the aforementioned control light to the 1st territory which should detect the inside aforementioned chemiluminescence of the observation aspect of the aforementioned organism sample, in the claim 1 which features that lighting pattern of the aforementioned control light is demarcated the microscope of statement.
[claim4]
4. As for the aforementioned control light, the 2nd control light which controls the formation of the aforementioned chemiluminescence the 1st control light which promotes the formation of the aforementioned chemiluminescence with lighting and with lighting implication,
In order to irradiate the aforementioned 2nd control light to the 2nd territory where the aforementioned demarcation section irradiates the aforementioned 1st control light to the 1st territory which should detect the inside aforementioned chemiluminescence of the observation aspect of the aforementioned organism sample, surrounds the aforementioned 1st territory, in the claim 1 which features that lighting pattern of the aforementioned 1st control light and the aforementioned 2nd control light is demarcated the microscope of statement.
[claim5]
5. Wave length of the aforementioned chemiluminescence it changes the aforementioned control light, to 2nd wave length from 1st wave length with the lighting,
The aforementioned demarcation section in order to irradiate the aforementioned control light to the 1st territory which should detect the inside aforementioned chemiluminescence of the observation aspect of the aforementioned organism sample, demarcates the lighting pattern of the aforementioned control light,
As for the aforementioned detector, without making the light of the aforementioned 1st wave length through the filter which makes the light of the aforementioned 2nd wave length transmit, in the claim 1 which features that the aforementioned chemiluminescence which is begun shooting from the aforementioned organism sample is detected the microscope of statement.
[claim6]
6. As for the aforementioned demarcation section, in order to form the plural aforementioned 1st territories on the aforementioned observation surface, the claim either 2 which features that lighting pattern of the aforementioned control light is demarcated or 5 in 1 sections the microscope of statement.
[claim7]
7. Processing the detection result of the aforementioned detector, the claim either 1 which features that furthermore it has the processing section which forms the picture of aforementioned chemiluminescence or 6 in 1 sections the microscope of statement.
[claim8]
8. The processing section which processes the detection result of the aforementioned detector furthermore having,
The aforementioned demarcation section demarcates lighting pattern of the aforementioned control light which is irradiated to the observation aspect of the aforementioned organism sample to 1st pattern,
The aforementioned detector detects the 2nd pattern which was formed to the aforementioned observation aspect by the interaction of the frequency component of the aforementioned organism sample and the frequency component of the aforementioned 1st pattern,
As for the aforementioned processing section, in the claim 1 which features that the frequency component of the aforementioned organism sample is sought on the basis of with the frequency component of the aforementioned 1st pattern and the frequency component of the aforementioned 2nd pattern the microscope of statement.
[claim9]
9. As for the aforementioned 1st pattern, implication pattern of stripe condition,
As for the aforementioned 2nd pattern, in the claim 8 which features that the moire which is obtained by the mutual interference of the frequency component of the aforementioned organism sample and the frequency component of the aforementioned 1st pattern is included the microscope of statement.
[claim10]
10. As for the aforementioned control light, 1W/cm (2) the claim either 1 which features that it possesses the strength below or 9 in 1 sections the microscope of statement.
[claim11]
11. The claim either 1 which features that furthermore it has the 2nd illuminant which ejects the stimulus light which stimulates the aforementioned organism sample or 10 in 1 sections the microscope of statement.
[claim12]
12. The luminous control section where the aforementioned chemiluminescence substance, includes the luminous substrate and the luminous enzyme and the fluorescent protein and the aforementioned luminous substrate cooperates and the luminescence protein fusion body and the aforementioned luminous enzyme which form the aforementioned chemiluminescence is connected, controls the aforementioned chemiluminescence according to the presence of the illumination of the aforementioned control light and, the claim either 1 which features that it includes or 11 in 1 sections the microscope of statement.
[claim13]
13. As for the aforementioned luminescence protein fusion body, to be able take with the 1st three-dimensional structure which generates the chemiluminescence reaction and the 2nd three-dimensional structure where occurrence of the aforementioned chemiluminescence reaction is controlled,
As for the aforementioned control light, three-dimensional structure of the aforementioned luminescence protein fusion body in the claim 12 which features that from the aforementioned 1st three-dimensional structure it changes to the aforementioned 2nd three-dimensional structure the microscope of statement.
[claim14]
14. As for the aforementioned luminous control section, Light-oxygen-voltage-sensing domain of huototoropin 2 (LOV2 domain), the AppA domain of LOV2-I427V and the BLUF protein which are the mutant of the aforementioned LOV2 domain (1-133), PapB domain of the BLUF protein (1-147), or, YcgF domain of the BLUF protein (1-97) the claim either 12 which features that it includes or 13 in 1 sections the microscope of statement.
[claim15]
15. As for the aforementioned luminescence protein fusion body, iero - nano- lanthanum, the cyanide - nano- lanthanum, the orange - nano- lanthanum, the green - nano- lanthanum, and the lead-lead - the claim either 12 which features that at least one of nano- lanthanum is included or 14 in 1 sections the microscope of statement.
[claim16]
16. The claim either 12 which features that the addition section which adds the aforementioned luminous substrate to the aforementioned organism sample furthermore is included or 15 in 1 sections the microscope of statement.
[claim17]
17. As for the aforementioned demarcation section, the Voltex phase plate and the spatial optical modulator, or, the claim either 1 which features that it is the transparent board where the material which cuts off the aforementioned control light was applied partly or 16 in 1 sections the microscope of statement.
[claim18]
18. The retention section which keeps the aforementioned organism sample and,
The shutter which it turns on turns off incidence to the aforementioned demarcation section of the aforementioned control light which is begun shooting from the aforementioned illuminant and,
The object glass which is arranged with the aforementioned retention section and the aforementioned detector and,
Furthermore the claim 1 which features that it has or either of 17 in 1 sections the microscope of statement.
[claim19]
19. As for the aforementioned detector, in the claim 18 which features that the aforementioned chemiluminescence which is begun shooting from the aforementioned observation aspect the side which is opposite to the side of the aforementioned demarcation section of the aforementioned organism sample is detected the microscope of statement.
[claim20]
20. Directing the aforementioned chemiluminescence where it is arranged with the aforementioned illuminant and the aforementioned retention section, directing the aforementioned control light which is begun shooting from the aforementioned illuminant to the aforementioned retention section, it leads, is begun shooting from the aforementioned observation aspect of side of the aforementioned demarcation section to the aforementioned detector, in the claim 18 which features that furthermore it has bimusupuritsuta which it leads the microscope of statement.
[claim21]
21. As for the aforementioned retention section, the optical path of the aforementioned control light in the direction which crosses vis-a-vis the aforementioned demarcation section movement the claim either 18 which features that it is possible or 20 in 1 sections the microscope of statement.
[claim22]
22. As for the aforementioned control light, the claim either 1 which features that it possesses the wave length of 380-480nm of 1 photon absorption or the wave length of 850-940nm of 2 photon absorption or 21 in 1 sections the microscope of statement.
[claim23]
23. As for the aforementioned organism sample, implication the chemiluminescence substance of the plural types which form the plural chemiluminescences of the wave length which differs mutually respectively,
As for the aforementioned detector, the claim either 1 which features that the aforementioned plural chemiluminescences are detected or 22 in 1 sections the microscope of statement.
[claim24]
24. The wave length filter which separates the aforementioned plural chemiluminescences on the basis of wave length furthermore having,
As for the aforementioned detector, in the claim 23 which features that each one of the aforementioned plural chemiluminescences which are separated by the aforementioned wave length filter is detected the microscope of statement.
[claim25]
25. Being the method of observing the organism sample in existence state,
The luminous control section which controls the aforementioned chemiluminescence according to the presence of the illumination of the control light where it includes the luminous enzyme and the fluorescent protein and the luminous substrate cooperates and the luminescence protein fusion body and the aforementioned luminous enzyme which form chemiluminescence is connected, changes the state of the aforementioned chemiluminescence and, the addition process which adds the aforementioned luminous substrate to the aforementioned organism sample which is included and,
The illumination process which illuminates the aforementioned organism sample with the aforementioned control light which demarcates the lighting pattern of the aforementioned control light, is demarcated to the aforementioned lighting pattern and,
The detection process which detects the aforementioned chemiluminescence which is begun shooting from the aforementioned organism sample and,
The method of featuring that it includes.
[claim26]
26. The aforementioned control light controls the formation of the aforementioned chemiluminescence with the lighting,
With the aforementioned illumination process, in order to the 1st territory which should detect the inside aforementioned chemiluminescence of the observation aspect of the aforementioned organism sample without irradiating the aforementioned control light, to irradiate the aforementioned control light to the 2nd territory which surrounds the aforementioned 1st territory, in the claim 25 which features that lighting pattern of the aforementioned control light is demarcated method of statement.
[claim27]
27. The aforementioned control light promotes the formation of the aforementioned chemiluminescence with the lighting,
With the aforementioned illumination process, in order to irradiate the aforementioned control light to the 1st territory which should detect the inside aforementioned chemiluminescence of the observation aspect of the aforementioned organism sample, in the claim 25 which features that lighting pattern of the aforementioned control light is demarcated method of statement.
[claim28]
28. As for the aforementioned control light, the 2nd control light which controls the formation of the aforementioned chemiluminescence the 1st control light which promotes the formation of the aforementioned chemiluminescence with lighting and with lighting implication,
In order to irradiate the aforementioned 2nd control light to the 2nd territory which with the aforementioned illumination process, irradiates the aforementioned 1st control light to the 1st territory which should detect the inside aforementioned chemiluminescence of the observation aspect of the aforementioned organism sample, surrounds the aforementioned 1st territory, in the claim 25 which features that lighting pattern of the aforementioned 1st control light and the aforementioned 2nd control light is demarcated method of statement.
[claim29]
29. Wave length of the aforementioned chemiluminescence it changes the aforementioned control light, to 2nd wave length from 1st wave length with the lighting,
With the aforementioned illumination process, in order to irradiate the aforementioned control light to the 1st territory which should detect the inside aforementioned chemiluminescence of the observation aspect of the aforementioned organism sample, lighting pattern of the aforementioned control light is demarcated,
With the aforementioned detection process, without making the light of the aforementioned 1st wave length through the filter which makes the light of the aforementioned 2nd wave length transmit, in the claim 25 which features that the aforementioned chemiluminescence which is begun shooting from the aforementioned organism sample is detected method of statement.
[claim30]
30. With the aforementioned illumination process, lighting pattern of the aforementioned control light which is irradiated to the observation aspect of the aforementioned organism sample is demarcated to 1st pattern,
With the aforementioned detection process, the 2nd pattern which was formed to the aforementioned observation aspect by the interaction of the frequency component of the aforementioned organism sample and the frequency component of the aforementioned 1st pattern is detected, in the claim 25 which features that the frequency component of the aforementioned organism sample is sought on the basis of with the frequency component of the aforementioned 1st pattern and the frequency component of the aforementioned 2nd pattern method of statement.
[claim31]
31. Implication the modification process which modifies the position of the aforementioned organism sample in the direction which crosses in the optical path of the aforementioned control light,
After the aforementioned modification process, the aforementioned illumination process and the aforementioned detection process the claim either 25 which features that repeatedly it does in the order or 30 in 1 sections method of statement.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • NAGAI TAKEHARU
  • ARAI YOSHIYUKI
  • SUZUKI KAZUSHI
国際特許分類(IPC)
指定国 (WO2017164332)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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