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ALGINATE LYASE AND METHOD USING ENZYME FOR PRODUCING UNSATURATED URONIC ACID MONOSACCHARIDE NEW

外国特許コード F170009261
整理番号 (S2016-0651-N0)
掲載日 2017年10月24日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP013871
国際公開番号 WO 2017175694
国際出願日 平成29年4月3日(2017.4.3)
国際公開日 平成29年10月12日(2017.10.12)
優先権データ
  • 特願2016-075364 (2016.4.4) JP
発明の名称 (英語) ALGINATE LYASE AND METHOD USING ENZYME FOR PRODUCING UNSATURATED URONIC ACID MONOSACCHARIDE NEW
発明の概要(英語) [Problem] To provide a novel alginate lyase and a method for producing an unsaturated uronic acid monosaccharide using the enzyme. [Solution] The present invention is achieved by a polypeptide that has an alginate lyase activity and that has an amino acid sequence represented by SEQ ID NO: 1 or an amino acid sequence sharing at least 90% homology with said sequence. This polypeptide is used in a method for producing 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH), the method comprising: (a) an enzyme addition step for bringing said polypeptide into contact with an alginic acid containing a uronic portion; and (b) an enzymatic action step for keeping the mixture of the alginic acid and the polypeptide at a temperature at which exo-type alginate lyase activity remains active.
特許請求の範囲(英語) [claim1]
1. Amino acid arrangement or the particular amino acid arrangement which is shown in arrangement number 1 being something which has the amino acid arrangement which at least possesses homology of 90% or more, the polypeptide which possesses ekiso type alginic acid lyase activity.
[claim2]
2. In claim 1 DNA which the polypeptide of statement the cord/code is done.
[claim3]
3. In claim 2 DNA of statement the vector for revelation which in claim 1 possesses the polypeptide of statement in revelation possible state.
[claim4]
4. (A) The enzyme addition process which makes the polypeptide of statement claim contact 1 vis-a-vis the alginic acid which includes the uron acid part, and,
(B) The aforementioned alginic acid and the blend of the polypeptide the enzyme action process which is kept at the temperature where the aforementioned ekiso type alginic acid lyase activity works and,
4-deoxy-L-erythro-5-hexoseulose uronic acid which it has (DEH) the manufacturing system.
[claim5]
5. Amino acid arrangement or the particular amino acid arrangement which is shown in arrangement number 2 being something which has the amino acid arrangement which at least possesses homology of 90% or more, the polypeptide which possesses ended type alginic acid lyase activity.
[claim6]
6. In claim 5 DNA which the polypeptide of statement the cord/code is done.
[claim7]
7. In claim 6 DNA of statement the vector for revelation which in claim 5 possesses the polypeptide of statement in revelation possible state.
[claim8]
8. (C) The plural enzyme addition processes which make the polypeptide of two types of polypeptide of statement and polypeptide which possesses ended type alginic acid lyase activity claim contact 1 vis-a-vis the alginic acid which includes the uron acid part, and,
(D) The blend of the polypeptide of the aforementioned alginic acid and two types the plural enzyme action processes which are kept at the temperature where the aforementioned alginic acid lyase activity works and,
The manufacturing system of DEH which it has.
[claim9]
9. The polypeptide which possesses the aforementioned ended type alginic acid lyase activity, in the claim 8 which is the polypeptide of statement in claim 5 the manufacturing system of DEH of statement.
[claim10]
10. HPLC which possesses with the formic acid content ammonium buffer of kinds as a column and a portable phase for anion analysis (the high performance liquid chromatograph) for DEH measurement LC-MS which has the section (the liquid chromatograph mass spectrometer) the device.
[claim11]
11. LC/MS which uses the column for anion analysis HPLC (the high performance liquid chromatography) in, uses the formic acid content formic acid ammonium buffer of kinds as a portable phase (liquid chromatography mass spectrometry) the measuring method of DEH which is used.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • MIE UNIVERSITY
  • 発明者(英語)
  • SHIBATA TOSHIYUKI
  • MIYAKE HIDEO
  • MURASE YOSHIHIRO
  • TANAKA REIJI
  • MORI TETSUSHI
  • TAKEYAMA HARUKO
  • TAKAHASHI MAMI
国際特許分類(IPC)
指定国 (WO2017175694)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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