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MARKER FOR IDENTIFYING GERM CELLS HAVING ENGRAFTABILITY AT HOST GONAD NEW

外国特許コード F170009269
整理番号 (S2016-0498-N0)
掲載日 2017年10月24日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP012112
国際公開番号 WO 2017164391
国際出願日 平成29年3月24日(2017.3.24)
国際公開日 平成29年9月28日(2017.9.28)
優先権データ
  • 特願2016-060914 (2016.3.24) JP
発明の名称 (英語) MARKER FOR IDENTIFYING GERM CELLS HAVING ENGRAFTABILITY AT HOST GONAD NEW
発明の概要(英語) The present invention relates to a marker for identifying germ cells, comprising (I) a marker for identifying germ cells having engraftability at a host gonad and/or (II) a marker for identifying germ cells not having engraftability at a host gonad. The present invention provides a germ cell marker that can identify the presence or absence of engraftability at a host gonad in fish.
特許請求の範囲(英語) [claim1]
1. The reproduction cell identification marker which possesses the raw arrival talent to the landlord gonad below-mentioned (i) and/or including the reproduction cell identification marker which does not possess the raw arrival talent to the landlord gonad (ii), it becomes, the reproduction cell identification marker:
(i) Description below (a) the - (f) from the reproduction cell identification marker which consists of or more where it is selected the polynucleotide of 1 or 2:
(A) The polynucleotide which consists of or more where it is selected from nucleotide arrangement of the arrangement number 1-74 the nucleotide arrangement of 1 or 2,
(B) Including or more where it is selected from nucleotide arrangement of the arrangement prayer number 1-74, the nucleotide arrangement of 1 or 2 being the polynucleotide which becomes, the polynucleotide which possesses the reproduction cell identification function which possesses the raw arrival talent to the landlord gonad,
(C) The nucleotide arrangement where 1 or several bases is deficient at the time of or more being selected from nucleotide arrangement of the arrangement number 1-74 nucleotide arranging of 1 or 2, addition, is inserted or is substituted, and/or on the other hand or being the polynucleotide which is displayed with the nucleotide arrangement which can do addition of the base to both ends, the polynucleotide which possesses the reproduction cell identification function which possesses the raw arrival talent to the landlord gonad,
(D) Or more where it is selected from nucleotide arrangement of the arrangement number 1-74 nucleotide arrangement of 1 or 2 being the polynucleotide which is displayed with the nucleotide arrangement which at least possesses 70% identity, the polynucleotide which possesses the reproduction cell identification function which possesses the raw arrival talent to the landlord gonad,
(E) The polynucleotide which the ortho log gene of the gene which consists of or more where it is selected from nucleotide arrangement of the arrangement number 1-74 the nucleotide arrangement of 1 or 2 the cord/code is done, and
(F) The description above (a) the - (e) the polynucleotide which consists of the complimentary arrangement of the polynucleotide which possesses the nucleotide arrangement which is shown to in each case high sutorinjiento being the polynucleotide which haiburidaizu is done under condition, the polynucleotide which possesses the reproduction cell identification function which possesses the raw arrival talent to the landlord gonad,
(ii) Description below (g) the - (l) from the reproduction cell identification marker which does not possess the raw arrival talent to the landlord gonad which consists of or more where it is selected the polynucleotide of 1 or 2:
(G) The polynucleotide which consists of or more where it is selected from nucleotide arrangement of the arrangement number 75-101 the nucleotide arrangement of 1 or 2,
(H) Including or more where it is selected from nucleotide arrangement of the arrangement prayer number 75-101, the nucleotide arrangement of 1 or 2 being the polynucleotide which becomes, the polynucleotide which possesses the reproduction cell identification function which does not possess the raw arrival talent to the landlord gonad,
(i) The nucleotide arrangement where 1 or several bases is deficient at the time of or more being selected from nucleotide arrangement of the arrangement number 75-101 nucleotide arranging of 1 or 2, addition, is inserted or is substituted, and/or on the other hand or being the polynucleotide which is displayed with the nucleotide arrangement which can do addition of the base to both ends, the polynucleotide which possesses the reproduction cell identification function which does not possess the raw arrival talent to the landlord gonad,
(J) Or more where it is selected from nucleotide arrangement of the arrangement number 75-101 nucleotide arrangement of 1 or 2 being the polynucleotide which is displayed with the base arrangement which at least possesses 70% identity, the polynucleotide which possesses the reproduction cell identification function which does not possess the raw arrival talent to the landlord gonad,
(K) The polynucleotide which the ortho log gene of the gene which consists of or more where it is selected from nucleotide arrangement of the arrangement number 75-101 the nucleotide arrangement of 1 or 2 the cord/code is done, and
(L) The description above (g) the - (k) the polynucleotide which consists of the complimentary arrangement of the polynucleotide which possesses the nucleotide arrangement which is shown to in each case high sutorinjiento being the polynucleotide which haiburidaizu is done under condition, the polynucleotide which possesses the reproduction cell identification function which does not possess the raw arrival talent to the landlord gonad.
[claim2]
2. The reproduction cell identification marker, is the reproduction cell identification marker which identifies the presence of raw arrival talent to the landlord gonad, in claim 1 the marker of statement.
[claim3]
3. Description below (a) the - (f) from it consists of or more where it is selected the polynucleotide of 1 or 2, the reproduction cell identification marker, is the reproduction cell identification marker which possesses the raw arrival talent to the landlord gonad, in claim 1 or 2 the marker of statement:
(A) The polynucleotide which consists of or more where it is selected from nucleotide arrangement of the arrangement number 1-74 the nucleotide arrangement of 1 or 2,
(B) Including or more where it is selected from nucleotide arrangement of the arrangement prayer number 1-74, the nucleotide arrangement of 1 or 2 being the polynucleotide which becomes, the polynucleotide which possesses the reproduction cell identification function which possesses the raw arrival talent to the landlord gonad,
(C) The nucleotide arrangement where 1 or several bases is deficient at the time of or more being selected from nucleotide arrangement of the arrangement number 1-74 nucleotide arranging of 1 or 2, addition, is inserted or is substituted, and/or on the other hand or being the polynucleotide which is displayed with the nucleotide arrangement which can do addition of the base to both ends, the polynucleotide which possesses the reproduction cell identification function which possesses the raw arrival talent to the landlord gonad,
(D) Or more where it is selected from nucleotide arrangement of the arrangement number 1-74 nucleotide arrangement of 1 or 2 being the polynucleotide which is displayed with the nucleotide arrangement which at least possesses 70% identity, the polynucleotide which possesses the reproduction cell identification function which possesses the raw arrival talent to the landlord gonad,
(E) The polynucleotide which the ortho log gene of the gene which consists of or more where it is selected from nucleotide arrangement of the arrangement number 1-74 the nucleotide arrangement of 1 or 2 the cord/code is done, and
(F) The description above (a) the - (e) the polynucleotide which possesses the nucleotide arrangement which is shown to in each case high sutorinjiento being the polynucleotide which haiburidaizu is done under condition, the polynucleotide which possesses the reproduction cell identification function which possesses the raw arrival talent to the landlord gonad.
[claim4]
4. The reproduction cell identification marker which possesses the raw arrival talent to the landlord gonad, the spirit field trunk cell (SSC) is the marker, either of the claim 1-3 in one section the marker of statement.
[claim5]
5. Is produced the fact that haiburidaizu it does to the polynucleotide which consists of the complimentary arrangement of the polynucleotide or the said polynucleotide of statement in claim 4, the primer or the probe for SSC gene detection.
[claim6]
6. Description below (g) the - (l) from it consists of or more where it is selected the polynucleotide of 1 or 2, the reproduction cell identification marker, is the reproduction cell identification marker which does not possess the raw arrival talent to the landlord gonad, in claim 1 or 2 the marker of statement:
(G) The polynucleotide which consists of or more where it is selected from nucleotide arrangement of the arrangement number 75-101 the nucleotide arrangement of 1 or 2,
(H) Including or more where it is selected from nucleotide arrangement of the arrangement prayer number 75-101, the nucleotide arrangement of 1 or 2 being the polynucleotide which becomes, the polynucleotide which possesses the reproduction cell identification function which does not possess the raw arrival talent to the landlord gonad,
(i) The nucleotide arrangement where 1 or several bases is deficient at the time of or more being selected from nucleotide arrangement of the arrangement number 75-101 nucleotide arranging of 1 or 2, addition, is inserted or is substituted, and/or on the other hand or being the polynucleotide which is displayed with the nucleotide arrangement which can do addition of the base to both ends, the polynucleotide which possesses the reproduction cell identification function which does not possess the raw arrival talent to the landlord gonad,
(J) Or more where it is selected from nucleotide arrangement of the arrangement number 75-101 nucleotide arrangement of 1 or 2 being the polynucleotide which is displayed with the base arrangement which at least possesses 70% identity, the polynucleotide which possesses the reproduction cell identification function which does not possess the raw arrival talent to the landlord gonad,
(K) The polynucleotide which the ortho log gene of the gene which consists of or more where it is selected from nucleotide arrangement of the arrangement number 75-101 the nucleotide arrangement of 1 or 2 the cord/code is done, and
(L) The description above (g) the - (k) the polynucleotide which consists of the complimentary arrangement of the polynucleotide which possesses the nucleotide arrangement which is shown to in each case high sutorinjiento being the polynucleotide which haiburidaizu is done under condition, the polynucleotide which possesses the reproduction cell identification function which does not possess the raw arrival talent to the landlord gonad.
[claim7]
7. Presence of revelation of the gene of the reproduction cell identification marker which possesses the raw arrival talent to the said landlord gonad either of the claim 1-4 making use of the reproduction cell identification marker which possesses the raw arrival talent to the landlord gonad of statement in one section, and/or detecting,
From the testes or the ovary of the Pisces, there is revelation or the cell whose revelation is high is separated
Including thing, the reproduction cell production method of becoming, possessing the raw arrival talent to the landlord gonad.
[claim8]
8. Presence of revelation of the gene of the reproduction cell identification marker which does not possess the raw arrival talent to the said landlord gonad making use of the reproduction cell identification marker which does not possess the raw arrival talent to the landlord gonad of statement in claim 1, 2 or 6, and/or detecting,
From the testes or the ovary of the Pisces, there is revelation or the cell whose revelation is high is separated
Furthermore including thing, the reproduction cell production method of becoming, possessing the raw arrival talent to the landlord gonad of statement in claim 7.
[claim9]
9. The Pisces, are the salmon course Pisces or the saba course Pisces, the reproduction cell production method of possessing the raw arrival talent to the landlord gonad of statement in claim 7 or 8.
[claim10]
10. The reproduction cell identification antibody which either of the claim 1-4 the reproduction cell identification marker which possesses the raw arrival talent to the landlord gonad of statement in one section connects to the portion of the protein which the cord/code is done, possesses the raw arrival talent to the landlord gonad.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY
  • UNIVERSITY OF TSUKUBA
  • 発明者(英語)
  • HAYASHI MAKOTO
  • IWASAKI YOSHIKO
  • HAYASHI TETSUTARO
  • EBISAWA MASASHI
  • SASAGAWA YOHEI
  • YOSHIMURA MIKA
  • NIKAIDO ITOSHI
  • ICHIDA KENSUKE
  • YOSHIZAKI GORO
国際特許分類(IPC)
指定国 (WO2017164391)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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