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CELL SORTING METHOD AND FLOW CYTOMETRY AND CELL SORTER USING SAME NEW

外国特許コード F170009273
整理番号 IP02P004
掲載日 2017年10月31日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP077645
国際公開番号 WO 2017163463
国際出願日 平成28年9月20日(2016.9.20)
国際公開日 平成29年9月28日(2017.9.28)
優先権データ
  • 特願2016-061233 (2016.3.25) JP
発明の名称 (英語) CELL SORTING METHOD AND FLOW CYTOMETRY AND CELL SORTER USING SAME NEW
発明の概要(英語) [Problem] To provide an automatable cell acquisition technique by which a plurality of cells can be simultaneously analyzed while reducing the frequencies of false positives and false negatives. [Solution] A cell sorter or flow cytometer comprising a flow passage provided with a plurality of microwells, an introduction passage capable of introducing a liquid, said liquid containing a plurality of target cells, into the flow passage, a data acquisition part acquiring data from the target cells housed in the microwells, a selective taking-out means capable of selectively taking out the target cells, said target cells being in the microwells, from the microwells on the basis of the data acquired by the data acquisition part, and a target cell collection part capable of collecting the target cells selectively taken out by the selective taking-out means, characterized in that: the microwells can house the target cells and a photodegradable gel; and the selective taking-out means is a degrading light-irradiation part capable of irradiating the photodegradable gel, said photodegradable gel being in a microwell housing a target cell to be taken out, with light having a wavelength at which the photodegradable gel degrades.
特許請求の範囲(英語) [claim1]
1. The passage which possesses the plural micro wells and,
The liquid which contains the plural suffering inspection bodies, in the aforementioned passage the introduction possible introduction road and,
The information acquisition section which acquires information from the plural suffering inspection bodies which are received to the aforementioned plural micro wells and,
The suffering inspection body inside one micro well is removed from inside the particular micro well selectively, on the basis of the information which the aforementioned information acquisition section acquires, possible selective removal expedient and,
The suffering inspection body which is removed selectively by the aforementioned selective removal expedient the collection possible suffering inspection body collection section
Being the cell sorter or flow sight meter which it possesses,
The aforementioned plural micro wells, the suffering inspection body and the disassembly characteristic gel are receipt possible,
The aforementioned selective removal expedient, is the disassembly processing section which administers the processing which the particular disassembly characteristic gel disassembles vis-a-vis the disassembly characteristic gel inside the micro well where the suffering inspection body which it should you remove is kept,
The way the cell sorter or flow sight meter which is formed.
[claim2]
2. The aforementioned passage is plane, the aforementioned plural micro wells below forming the particular passage, are provided in the wall, the cell sorter or flow sight meter of claim 1 statement.
[claim3]
3. The aforementioned disassembly characteristic gel, is the photolysis characteristic gel,
The aforementioned disassembly processing section light of the wave length which the aforementioned photolysis characteristic gel disassembles is the lighting possible optical lighting section, claim the cell sorter or flow sight meter of 1 or 2 statements.
[claim4]
4. As for the aforementioned information acquisition section,
At one time it is acquisition possible at one time concerning acquisition or the aforementioned plural all micro wells the aforementioned information from the aforementioned plural micro wells, in micro every well concerning those of 2 or more inside acquisition and the plural micro wells, either of the claim 1-3 the cell sorter or flow sight meter of one section statement.
[claim5]
5. As for the aforementioned information acquisition section,
Vis-a-vis the aforementioned plural micro wells, the information source wave which becomes the source of the aforementioned information which it should acquire the lighting possible information source wave lighting section and,
The aforementioned information source wave lighting section the occasion where the aforementioned information source wave was irradiated vis-a-vis the aforementioned plural micro wells, the information reception section which receives the aforementioned information which can occur from the aforementioned plural micro wells
It possesses, either of the claim 1-4 the cell sorter or flow sight meter of one section statement.
[claim6]
6. Is the aforementioned information source wave lighting section the aforementioned information source wave lighting possible in micro every well as a first feature?;
As a second feature, those of 2 or more among the plural micro wells it is lighting possible at one time?;
Or, the aforementioned plural all micro wells it is lighting possible at one time as a third feature;
In case of the aforementioned first feature and the aforementioned second feature, scanning the aforementioned plural micro well everything, it can irradiate, the cell sorter or flow sight meter of claim 5 statement.
[claim7]
7. The aforementioned information source wave lighting section the information source wave of plural kinds is output enable according to the number of analytical items, claim the cell sorter or flow sight meter of 5 or 6 section statements.
[claim8]
8. The aforementioned information source wave which is irradiated from the aforementioned information source wave lighting section is light, the aforementioned disassembly characteristic gel is the photolysis characteristic gel, at the same time, the aforementioned disassembly processing section, differs from the wave length of the light where when it is the lighting possible optical lighting section, as for wave length of particular light, the aforementioned photolysis characteristic gel disassembles the light of the wave length which the aforementioned photolysis characteristic gel disassembles, either of the claim 5-7 the cell sorter or flow sight meter of one section statement.
[claim9]
9. The aforementioned optical lighting section and the aforementioned information source wave lighting section, are the identical illuminant,
The aforementioned identical illuminant becomes light of the wave length which the aforementioned photolysis characteristic gel disassembles and the source of the aforementioned information which it should acquire, the aforementioned wave length which the aforementioned photolysis gel disassembles light of the wave length which differs and, change lighting possibly it is constituted, the cell sorter or flow sight meter of claim 8 statement.
[claim10]
10. As for the aforementioned cell sorter or flow sight meter,
-description above information acquire section with acquire do description above information automatic operation analyze do automatic operation analyze expedient possess be good,
Furthermore, in order to administer the processing which the particular gel disassembles in the disassembly characteristic gel inside the micro well where the suffering inspection body where acquisition is desired on the basis of the analytical result due to indication of the user or aforementioned automatic analytical expedient, is received, the selective removal control means which control the aforementioned selective removal expedient
Furthermore it possesses, either of the claim 1-9 the cell sorter or flow sight meter of one section statement.
[claim11]
11. Either of the claim 1-10 it is the cell sorter or for flow sight meter of one section statement, the micro fluid device which has the aforementioned passage.
[claim12]
12. Either of the claim 1-10 it is the cell sorter or for flow sight meter of one section statement, the liquid which contains the material which can form the aforementioned disassembly characteristic gel.
[claim13]
13. The filling up process which in the passage which possesses the plural micro wells, lets flow the liquid which contains the suffering inspection body, fills up the aforementioned suffering inspection body inside the aforementioned micro well and,
The description above the analytical process which analyzes the aforementioned suffering inspection body which fills up after the filling up process and inside the aforementioned micro well and,
The description above after the analytical process, the acquisition process which removes the aforementioned suffering inspection body
Being the suffering inspection body collection method of including,
The aforementioned liquid contains, the first component and the aforementioned first component the second component which differs and,
At the same time the aforementioned first component, the basic framework is the organism compatibility polymer and possessing the part which it can connect with the aforementioned second component,
At the same time the aforementioned second component, the basic framework is the organism compatibility polymer and possessing the second section which it can connect with the aforementioned first component,
The case of the aforementioned analytical process, the gel where the aforementioned first component and the aforementioned second component build a bridge with the connection formation of the aforementioned part and the aforementioned second section, is a state where it is formed inside the aforementioned micro well,
The aforementioned first component and/or the aforementioned second component furthermore has had the disassembly characteristic part,
Regarding the aforementioned acquisition process, by the fact that disassembly processing is done, making the aforementioned disassembly characteristic part disassemble, it disassembles the aforementioned gel, removes the aforementioned suffering inspection body, suffering inspection body collection method.
[claim14]
14. The aforementioned part is azide, the aforementioned second section is alkyne, suffering inspection body collection method of claim 13 statement.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • YAMAGUCHI SATOSHI
  • OKAMOTO AKIMITSU
  • TAKAGI RISA
  • NOJI HIROYUKI
  • IINO RYOTA
  • TABATA KAZUHITO
国際特許分類(IPC)
指定国 (WO2017163463)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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