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METHOD FOR PRODUCING CULTIVATED EPITHELIAL CELL SHEET NEW

外国特許コード F170009283
整理番号 (S2016-0583-N0)
掲載日 2017年11月7日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP015704
国際公開番号 WO 2017183655
国際出願日 平成29年4月19日(2017.4.19)
国際公開日 平成29年10月26日(2017.10.26)
優先権データ
  • 特願2016-084471 (2016.4.20) JP
発明の名称 (英語) METHOD FOR PRODUCING CULTIVATED EPITHELIAL CELL SHEET NEW
発明の概要(英語) A method for producing an epithelial cell sheet, said method comprising a step for cultivating cells, which are derived from oral mucosal epithelial cells, in a serum-free medium on a substrate, wherein the serum-free medium contains: (i) EGF protein or KGF protein; (ii) a B-27 supplement; and (iii) an ROCK inhibitor.
特許請求の範囲(英語) [claim1]
1. Being production method of the epithelium cell seat which includes the process which the cell of buccal cavity mucous membrane epithelium cell origin, bloodless clearly is cultured in the nutrient medium and on the backing material, the aforementioned bloodless is clear the nutrient medium
(i) EGF protein or KGF protein,
(ii) B-27 supplement, and
(iii) ROCK inhibiter
Bloodless it includes clearly it is the nutrient medium, method.
[claim2]
2. The above-mentioned nutrient medium,
(iv) Polysaccharide,
(v) katekin, and
(vi) Corticoid
In the claim 1 which at least furthermore includes 1 where it is selected from the group which consists of kinds method of statement.
[claim3]
3. In claim 1 or 2 the epithelium cell seat which is obtained by the method of statement.
[claim4]
4. (i) EGF protein or KGF protein,
(ii) B-27 supplement, and
(iii) ROCK inhibiter
It includes, bloodless in order the epithelium cell seat to produce the cell of buccal cavity mucous membrane epithelium cell origin as a material it is clear the nutrient medium.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KYOTO PREFECTURAL PUBLIC UNIVERSITY
  • 発明者(英語)
  • NAKAMURA TAKAHIRO
  • KINOSHITA SHIGERU
  • YOKOO SEIICHI
  • YAMAGAMI SATORU
国際特許分類(IPC)
指定国 (WO2017183655)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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