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ESTABLISHMENT OF SYNTHESIS METHOD FOR CYSTEINE WITH ISOTOPICALLY LABELED SULFUR ATOM AND CYSTEINE DERIVATIVE NEW

外国特許コード F170009289
整理番号 (S2016-0715-N0)
掲載日 2017年11月14日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP016654
国際公開番号 WO 2017188355
国際出願日 平成29年4月27日(2017.4.27)
国際公開日 平成29年11月2日(2017.11.2)
優先権データ
  • 特願2016-090864 (2016.4.28) JP
発明の名称 (英語) ESTABLISHMENT OF SYNTHESIS METHOD FOR CYSTEINE WITH ISOTOPICALLY LABELED SULFUR ATOM AND CYSTEINE DERIVATIVE NEW
発明の概要(英語) The present invention relates to synthesis methods for cysteine with an isotopically labeled sulfur atom and a cysteine derivative. The present invention also relates to a kit for use in the synthesis methods. The present invention makes it possible to easily and efficiently synthesize cysteine with an isotopically labeled sulfur atom and a cysteine derivative.
特許請求の範囲(英語) [claim1]
1. The sulfur atom being synthetic method of the cystein which the isotopic labeling is done, cystein synthetase (EC: 2.5.1.47) Under existing, the process which reacts the substrate which the isotopic labeling is done is included concerning O-ASECHIRU-L-SERIN and the sulfur atom, the substrate which the isotopic labeling is done the hydrogen sulfide sodium (NaHS), the sodium sulfide (Na [2] S), or the hydrogen sulfide (H [2] S) is here concerning the particular sulfur atom, and here the isotopic labeling of the particular sulfur atom, the stable isotope (32) S and the stable isotope (34) S and the radioisotope (33) S and the radioisotope (35) is selected from the group which consists of S, the aforementioned method.
[claim2]
2. O-ASECHIRU-L-SERIN, process below: Ceric - O-asechirutoransuhueraze (EC: 2.3.1.30) Under existing, it reacts ceric the isotopic labeling it is done nitrogen and/or concerning the carbon atom;
The fact that you obtain with furthermore is included, in claim 1 method of statement.
[claim3]
3. Cystein synthetase is, cystein synthetase A (CysK) or cystein synthetase B (CysM), in claim 1 or 2 method of statement.
[claim4]
4. Cystein synthetase A, is the microbial origin which belongs to Salmonella being attached, in claim 3 method of statement.
[claim5]
5. Cystein synthetase, is the rearrangement protein which includes the histidine tag, either of the claim 1-4 in 1 sections method of statement.
[claim6]
6. The sulfur atom being synthetic method of the cystein which the isotopic labeling is done, following to the method of statement in claim, 1 or 2 the sulfur atom it synthesizes the cystein which the isotopic labeling is done, it includes the process which obtains cystein by oxidizing particular cystein under existing of the oxidizer, the aforementioned method.
[claim7]
7. The oxidizer is iodine (I [2]), in claim 6 method of statement.
[claim8]
8. Being synthetic method of the cystein derivative,
(i) The cystein derivative, the sulfur atom is the sulfonic cystein which the isotopic labeling is done, under existing of cystein synthetase, O-ASECHIRU-L ceric and the sodium sulfite (Na [2] SO [3]) the process which it reacts is included, here the sulfur atom, the stable isotope (32) S and the stable isotope (34) S and the radioisotope (33) S and the radioisotope (35) is selected from the group which consists of S;
(ii) The cystein derivative, the sulfur atom is the S-suruho cystein which the isotopic labeling is done, under existing of cystein synthetase B, O-ASECHIRU-L-SERIN and the sodium thiosulfate (Na [2] S [2] O [3]) the process which it reacts is included, here the sulfur atom, the stable isotope (32) S and the stable isotope (34) S and the radioisotope (33) S and the radioisotope (35) is selected from the group which consists of S;
(iii) The cystein derivative, the sulfur atom is the cystein per sulfide which the isotopic labeling is done, under existing of cystein synthetase, O-ASECHIRU-L-SERIN and two sodium sulfide (Na [2] S [2]) the process which it reacts is included, here the sulfur atom, the stable isotope (32) S and the stable isotope (34) S and the radioisotope (33) S and the radioisotope (35) is selected from the group which consists of S;
(iv) The cystein derivative, the sulfur atom to be the cystein per sulfide which the isotopic labeling is done, (a) under existing of cystein synthetase, reacting the substrate which the isotopic labeling is done concerning O-ASECHIRU-L-SERIN and the sulfur atom and the sulfur atom the process which obtains the cystein which the isotopic labeling is done, the substrate which the isotopic labeling is done the hydrogen sulfide sodium (NaHS), or the sodium sulfide (Na [2] S) to be here concerning the particular sulfur atom, and here as for the isotopic labeling of the particular sulfur atom, the stable isotope (32) S and the stable isotope (34) S and the radioisotope (33) S and the radioisotope (35) it is selected from the group which consists of S, and (b) process (a) with the sulfur atom which is obtained is included the process which reacts cystein and two sodium sulfide which the isotopic labeling are done;
Or
(v) The cystein derivative, is serenoshisutein, under existing of cystein synthetase, O-ASECHIRU-L-SERIN and the selenium conversion sodium (Na [2] Se) the process which it reacts is included;
The aforementioned method.
[claim9]
9. O-ASECHIRU-L-SERIN, ceric - O-asechirutoransuhueraze (EC: 2.3.1.30) Under existing, it reacts ceric the isotopic labeling it is done nitrogen and/or concerning the carbon atom;
The fact that you obtain with furthermore is included, in claim 8 method of statement.
[claim10]
10. Cystein synthetase is, cystein synthetase A (CysK) or cystein synthetase B (CysM), in claim 8 or 9 method of statement.
[claim11]
11. Cystein synthetase A, is the microbial origin which belongs to Salmonella being attached, in claim 10 method of statement.
[claim12]
12. Cystein synthetase, is the rearrangement protein which includes the histidine tag, either of the claim 8-11 in 1 sections method of statement.
[claim13]
13. The sulfur atom being the kit in order to synthesize cystein or cystein or the cystein derivative which the isotopic labeling are done,
Cystein synthetase;
And
Concerning the sulfur atom the isotopic labeling the hydrogen sulfide sodium which is done (NaHS), the sodium sulfide (Na [2] S), the sodium sulfite (Na [2] SO [3]), the sodium thiosulfate (Na [2] S [2] O [3]), and two sodium sulfide (Na [2] S [2]) from the group which consists of the substrate which at least includes one which is selected sulfur, here as for the isotopic labeling of the particular sulfur atom, the stable isotope (32) S and the stable isotope (34) S and the radioisotope (33) S and radioactivityThe isotope (35) it is selected from the group which consists of S, or the selenium conversion sodium (Na [2] Se);
It includes, the aforementioned kit.
[claim14]
14. Being synthetic method of the cystein derivative,
(i) The cystein derivative, the sulfur atom to be the cystein polysulfide which the isotopic labeling is done, (a) following to the method of statement in claim and 1 or 2 the sulfur atom the process which synthesizes the cystein which the isotopic labeling it does, (b) process (a) with the process which reacts the cystein which the isotopic labeling it does with the oxidizer, and (c) furthermore sodium sulfide (Na [2] S) adding the sulfur atom which you obtain the process which it reacts, it includes;
Or
(ii) The cystein derivative, the sulfur atom is the N-asechiru cystein which the isotopic labeling is done, (a) you follow to the method of statement in claim and 1 or 2 the sulfur atom the process which synthesizes the cystein which the isotopic labeling is done, and (b) process (a) with the process which reacts the cystein which the isotopic labeling is done with the acetylation medicine, you include the sulfur atom which is obtained;
The aforementioned method.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KUMAMOTO UNIVERSITY
  • 発明者(英語)
  • SAWA TOMOHIRO
  • ONO KATSUHIKO
国際特許分類(IPC)
指定国 (WO2017188355)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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