PROBLEM TO BE SOLVED: To screen a substance which induces hepatpathy by measuring galeclin-3 inducibility.
SOLUTION: This substance screening method for giving a substance to be tested to a mammal except a human, lever tissue, or cultured hepatocytes and measuring gallectin-3 inducibility, a method for determining the condition of a liver by measuring gallectin-3 and the amount of a related substance thereof, and a kit utilizing these methods are provided. The detection of an increase of gallectin-3 in a liver is an index for determining the condition of drug-induced injuries of hepatocytes, the presence of inflammation of hepatocytes, their restoration, existence at restoration, and restorable hepatocytes thereafter. Its effect is generally applied not only to medication-induced and alcoholic hepatopathy but also to various types of infectious hepatitis. In general medication development, by measuring the gallectin-3 inducibility of compounds to be tested in hepatocytes, it is possible to easily screen and exclude highly hepatopathic compounds. In addition, the transcription of the gallectin-3 genes is accelerated by a natural transcription factor protein. Therefore, by chemically synthesizing a substance without toxicity which accelerates the synthesis of the gallectin-3 gene transcription factor protein in a liver, it is possible to use it as a therapeutic agent for carious types of hepatopathy including viral hepatitis.